Biocompatibility evaluation of alendronate paste in rat’s subcutaneous tissue

Abstract

Alendronate is a known inhibitor of root resorption and the development of alendronate paste would enhance its utilization as intracanal medication. Therefore, this study aimed to investigate the biocompatibility of experimental alendronate paste in subcutaneous tissue of rats, for utilization in teeth susceptible to root resorption. The study was conducted on 15 male rats, weighing approximately 180-200 grams. The rats' dorsal regions were submitted to one incision on the median region and, laterally to the incision, the subcutaneous tissue was raised and gently dissected for introduction of two tubes, in each rat. The tubes were sealed at one end with gutta-percha and taken as control. The tubes were filled with experimental alendronate paste. The animals were killed at 7, 15 and 45 days after surgery and the specimens were processed in laboratory. The histological sections were stained with hematoxylin-eosin and analyzed by light microscopy. Scores were assigned to the inflammatory process and statistically compared by the Tukey test (P < 0.05). Alendronate paste promoted severe inflammation process at 7 days, with statistically significant difference compared to the control (P < 0.05%). However, at 15 days, there was a regression of inflammation and the presence of connective tissue with collagen fibers, fibroblasts and blood vessels was observed. After 45 days, it was observed the presence of well-organized connective tissue, with collagen fibers and fibroblasts, and few inflammatory cells. No statistical difference was observed between the control and experimental paste at 15 and 45 days. The experimental alendronate paste was considered biocompatible with subcutaneous tissue of rat.

Biocompatibility of a Calcium Hydroxide-Propolis Experimental Paste in Rat Subcutaneous Tissue

“…A biocompatible material should present low toxicity without promoting inflammatory reaction (16,18,21), which should be insignificant when present (16,18,21). Silveira et al (22) stated that a material is considered biocompatible if the inflammatory reaction is reduced to insignificant along with time.…”

“…More specifically, implantation of materials in the subcutaneous tissue of small animals like rats is considered a reliable and adequate test to evaluate the biocompatibility of materials (17,18,(21)(22)(23). The material may be introduced in the tissues inside polyethylene tubes (17).…”

Análise comparativa da reação tecidual à implantação de tubos de polietileno, bastões de dentina e cápsulas de colágeno. Estudo microscópico em tecido subcutâneo de ratos. Avaliação de um modelo metodológico

“…POLLICK, et al, 1995;CANOVA, 2003;SCHWARZ, et al, 2007), carneiros (GOSAIN, et al, 2002;), coelhos (MAEDA, 2005, camundongos (SCHWARTZ, et al, 2000), cobaias (RUMPEL, et al, 2006), hamsters (LASCHKE, et al, 2007) e os ratos de laboratório, que segundo Hedrich (2000), são os animais experimentais mais comumente utilizados, pois é o modelo que melhor representa o funcionamento do sistema mamífero. Serve como modelo para o teste de um grande número de materiais para aplicação em Endodontia e Cirurgia (TORNECK, 1966;PHILLIPS, 1967;TORNECK, 1967;HORSTED;FEJERSKOV;JOHANNESEN, 1980;GORDUYSUS;ETIKAN;GOKOZ, 1998;HOLLAND, et al, 1999;2001;2002a;2002b;MORAIS, et al, 2006;ZAMBUZZI, et al, 2006;GARCIA, 2008;GOMES, et al, 2008;MORI, et al, 2009 (MAGRO, et al, 1987;GORDUYSUS;ETIKAN;GOKOZ, 1998;YALTIRIK, et al, 2004;CINTRA, et al, 2006;MORAIS, et al, 2006;SUMER, et al, 2006;MORI, et al, 2009), de teflon (OLSSON;SLIWKOWSKI;ECONOMIDES, et al, 1995;KOLOKOURIS, et al, 1996;, ...…”

“…Posteriormente, outros autores utilizaram também os tubos de polietileno, onde foi observada a ausência de inflamação frente ao material (MAGRO, et al, 1987;GORDUYSUS;ETIKAN;GOKOZ, 1998;YALTIRIK, et al, 2004;CINTRA, et al, 2006;MORAIS, et al, 2006;SUMER, et al, 2006;MORI, et al, 2009). Economides (1995), Kolokouris (1996; Holland et al (1999;2001;2002a;2002b) empregaram tubos de dentina preparados a partir de raízes de dentes humanos, para avaliação do MTA e cimento Portland em tecido subcutâneos de ratos.…”

“…Em geral, os implantes dos materiais endodônticos, em tecido conjuntivo, são realizados em tubos de polietileno (MAGRO, et al, 1987;GORDUYSUS;ETIKAN;GOKOZ, 1998;YALTIRIK, et al, 2004;CINTRA, et al, 2006;MORAIS, et al, 2006;SUMER, et al, 2006;MORI, et al, 2009). São utilizados também tubos de teflon SLIWKOWSKI;ECONOMIDES, et al, 1995;KOLOKOURIS, et al, 1996;, de silicone rígido (ZMENER; GUGLIELMOTTI;…”