Biocompatibility of Antimicrobial Melimine Lenses

Dutta, Debarun; Ozkan, Jerome; Willcox, Mark

doi:10.1097/opx.0000000000000232

Cited by 66 publications

(64 citation statements)

References 53 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In vivo melimine coatings demonstrated a significant reduction of CLPU incidence in the rabbit model [19]. The broad-spectrum antimicrobial activity of melimine was also confirmed in a human clinical trial, where melimine-coated lenses turned out to be safe for use during wear and retained a high antimicrobial activity after wear [20]. Other studies have shown that the melimine coating was heat-stable, non-toxic to mammalian cells in vitro, and did not alter the physical dimensions of CL [17,19,21].…”

Section: Discussionmentioning

confidence: 99%

“…Direct topical AMPs application, production of AMP’s derivatives and peptidomimetics, construction of specifically-targeted AMPs (STAMP), or immobilization of AMPs directly onto the surface of biomaterials are some of the examples [16,17]. Several in vitro and in vivo studies have been performed to investigate a group of AMPs as potential anti-biofilm agents for ocular infection therapies [17,18,19,20,21]. …”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Influence of Amphibian Antimicrobial Peptides and Short Lipopeptides on Bacterial Biofilms Formed on Contact Lenses

et al. 2016

View full text Add to dashboard Cite

The widespread use of contact lenses is associated with several complications, including ocular biofilm-related infections. They are very difficult to manage with standard antimicrobial therapies, because bacterial growth in a biofilm is associated with an increased antibiotic resistance. The principal aim of this study was to evaluate the efficacy of antimicrobial peptides (AMPs) in eradication of bacterial biofilms formed on commercially available contact lenses. AMPs were synthesized according to Fmoc/tBu chemistry using the solid-phase method. Minimum inhibitory concentration (MIC) and minimum biofilm eradication concentration (MBEC) of the compounds were determined. Anti-biofilm activity of the antimicrobial peptides determined at different temperatures (25 °C and 37 °C) were compared with the effectiveness of commercially available contact lens solutions. All of the tested compounds exhibited stronger anti-biofilm properties as compared to those of the tested lens solutions. The strongest activity of AMPs was noticed against Gram-positive strains at a temperature of 25 °C. Conclusions: The results of our experiments encourage us toward further studies on AMPs and their potential application in the prophylaxis of contact lens-related eye infections.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Influence of Amphibian Antimicrobial Peptides and Short Lipopeptides on Bacterial Biofilms Formed on Contact Lenses

et al. 2016

View full text Add to dashboard Cite

show abstract

“…Alternatively, implants and other medical devices can also reduce bacterial colonization, biofilm formation, and infection rates, presenting long lasting functionality, broad-spectrum activity, and minimal cytotoxicity against human cells. 276,277 Human AMPs could, theoretically, also be used as biosensors immobilized onto microchips for identification purposes, allowing the identification of microorganisms. However, such applications have only been exploited with animal or synthetic AMPs.…”

Section: Application Of Amps Beyond Conventional Pharmacological Therapymentioning

confidence: 99%

Human Antimicrobial Peptides in Bodily Fluids: Current Knowledge and Therapeutic Perspectives in the Postantibiotic Era

Bastos

Trindade

Costa

et al. 2017

Medicinal Research Reviews

View full text Add to dashboard Cite

Antimicrobial peptides (AMPs) are an integral part of the innate immune defense mechanism of many organisms. Due to the alarming increase of resistance to antimicrobial therapeutics, a growing interest in alternative antimicrobial agents has led to the exploitation of AMPs, both synthetic and isolated from natural sources. Thus, many peptide-based drugs have been the focus of increasing attention by many researchers not only in identifying novel AMPs, but in defining mechanisms of antimicrobial peptide activity as well. Herein, we review the available strategies for the identification of AMPs in human body fluids and their mechanism(s) of action. In addition, an overview of the distribution of AMPs across different human body fluids is provided, as well as its relation with microorganisms and infectious conditions.

show abstract

“…Risk factors for microbial keratitis include: ( i ) extended wear of CL; ( ii ) CL wear while swimming; ( iii ) improper storage and disinfection of lenses with tap water or home‐made solutions; ( iv ) wear of cosmetic CL, especially if purchased online or from roadside shops . Importantly, newer lens materials, care regimes, and wear modalities have not reduced the incidence and severity of infections .…”

Section: Introductionmentioning

confidence: 99%

Esculentin‐1a derived peptides kill Pseudomonas aeruginosa biofilm on soft contact lenses and retain antibacterial activity upon immobilization to the lens surface

et al. 2018

Self Cite

View full text Add to dashboard Cite

Contact lens (CL) wear is a risk factor for development of microbial keratitis, a vision threatening infection of the eye. Adverse events associated with colonization of lenses, especially by the multi-drug resistant and biofilm forming bacterium Pseudomonas aeruginosa remain a major safety issue. Therefore, novel strategies and compounds to reduce the onset of CL-associated ocular infections are needed. Recently, the activity of the frog skin-derived antimicrobial peptide Esc(1-21) and its diastereomer Esc(1-21)-1c was evaluated against both planktonic and sessile forms of this pathogen. Furthermore, Esc(1-21) was found to significantly reduce the severity of P. aeruginosa keratitis in a mouse model and preserve antipseudomonal activity in the presence of human basal tears. Here, we have analyzed the activity of the peptides on P. aeruginosa biofilm formed on soft CLs. Microbiological assays and scanning electron microscopy analysis indicated that the peptides were able to disrupt the bacterial biofilm, with the diastereomer having the greater efficacy (up to 85% killing vs no killing at 4 μM for some strains). Furthermore, upon covalent immobilization to the CL, the two peptides were found to cause more than four log reduction in the number of bacterial cells within 20 minutes and to reduce bacterial adhesion to the CL surface (77%-97% reduction) in 24 hours. Importantly, peptide immobilization was not toxic to mammalian cells and did not affect the lens characteristics. Overall, our data suggest that both peptides have great potential to be developed as novel pharmaceuticals for prevention and treatment of CL-associated P. aeruginosa keratitis.

show abstract

Biocompatibility of Antimicrobial Melimine Lenses

Abstract: Melimine-coated contact lenses were worn safely by humans. However, they were associated with higher corneal staining. The melimine-coated lenses retained high antibacterial activity after wear.

Cited by 66 publications

References 53 publications

Influence of Amphibian Antimicrobial Peptides and Short Lipopeptides on Bacterial Biofilms Formed on Contact Lenses

Influence of Amphibian Antimicrobial Peptides and Short Lipopeptides on Bacterial Biofilms Formed on Contact Lenses

Human Antimicrobial Peptides in Bodily Fluids: Current Knowledge and Therapeutic Perspectives in the Postantibiotic Era

Esculentin‐1a derived peptides kill Pseudomonas aeruginosa biofilm on soft contact lenses and retain antibacterial activity upon immobilization to the lens surface

Contact Info

Product

Resources

About