Biocompatibility of Contact Lens Solutions Using Confocal Laser Scanning Microscopy and the In Vitro Bovine Cornea

Bantseev, Vladimir; McCanna, David J.; Driot, Jean-Yves; Ward, Keith W.; Sivak, J. G.

doi:10.1097/icl.0b013e31803c55ad

Cited by 9 publications

(5 citation statements)

References 29 publications

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“…On the other hand, although there is some evidence of cytotoxicity in cell culture studies, in general the response is not accompanied by changes in cell morphology, metabolism or viability, membrane integrity or barrier function or the expression of apoptotic or inflammatory markers (Bantseev et al, 2007) This has suggested that transient hyperfluorescence is not adequately explained by and due to, uptake of fluorescein into damaged epithelial cells.…”

Section: Fluorescein Spread Within the Epitheliummentioning

confidence: 84%

Clinical staining of the ocular surface: Mechanisms and interpretations

Bron

Argüeso

İrkeç

et al. 2015

Progress in Retinal and Eye Research

140

109

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Section: Fluorescein Spread Within the Epitheliummentioning

confidence: 84%

Clinical staining of the ocular surface: Mechanisms and interpretations

Bron

Argüeso

İrkeç

et al. 2015

Progress in Retinal and Eye Research

140

109

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“…Additionally, most in vitro studies show PHMB‐based solutions have minimal toxicity, including those that used the most sensitive assays . Furthermore, a large proportion of studies has shown PHMB‐based solutions are less cytotoxic than those containing PQ‐1/Aldox, which show a lower intensity of SICS/PATH.…”

Section: Aetiology Of Sics/pathmentioning

confidence: 99%

Putting vital stains in context

Efron

2013

Clinical and Experimental Optometry

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While vital staining remains a cornerstone in the diagnosis of ocular disease and contact lens complications, there are many misconceptions regarding the properties of commonly used dyes by eye‐care practitioners and what is and what is not corneal staining after instillation of sodium fluorescein. Similarly, the proper use and diagnostic utility of rose Bengal and lissamine green B, the other two ophthalmic dyes commonly used for assessing ocular complications, have similarly remained unclear. Due to the limitations of vital stains for definitive diagnosis, concomitant signs and symptoms in addition to a complete patient history are required. Over the past decade, there have been many reports of a type of corneal staining—often referred to as solution‐induced corneal staining (SICS)—that is observed with the use of multipurpose solutions in combination with soft lenses, more specifically silicone hydrogel lenses. Some authors believe that SICS is a sign of lens/solution incompatibility; however, new research shows that SICS may be neither a measure of lens/solution biocompatibility nor ‘true’ corneal staining, as that observed in pathological situations. A large component of SICS may be a benign phenomenon, known as preservative‐associated transient hyperfluorescence (PATH). There is a lack of correlated signs and/or symptoms with SICS/PATH. Several properties of SICS/PATH, such as appearance and duration, differentiate it from pathological corneal staining. This paper reviews the properties of vital stains, their use and limitations in assessment of the ocular surface, the aetiology of corneal staining, characteristics of SICS/PATH that differentiate it from pathological corneal staining and what the SICS/PATH phenomenon means for contact lens‐wearing patients.

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“…The stained cornea, limbus, and bulbar conjunctiva was placed on a glass container and immobilized with 1% agarose gel previously melted in Hanks balanced salt solution, as described previously. 16 The staining was initiated immediately after dissection, and microscopy was performed within 3 hours after enucleation.…”

Section: Methodsmentioning

confidence: 99%

Corneal, Limbal, and Conjunctival Epithelium of Bovine Eyes Imaged In Vitro by Using a Confocal Laser Scanning Microscope

Feng

Bantseev²,

Simpson³

2008

Cornea

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Confocal results of the corneal central, limbal, and conjunctival morphology are similar to those found in traditional microscopic observations. Bovine central corneal epithelium is thicker than limbal epithelium. However, the nuclear AO staining pattern of unfixed ocular surface epithelium of bovine eyes in vitro might represent the cell differentiation status. With the aid of the fluorescence dye, confocal laser scanning microscopy can provide unique morphometric information about corneal, limbal, and conjunctival epithelial cells.

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Biocompatibility of Contact Lens Solutions Using Confocal Laser Scanning Microscopy and the In Vitro Bovine Cornea

Abstract: OPTI-FREE Express has the potential for deeper mitochondrial effects compared to ReNu MultiPlus No Rub and Hanks' balanced salt solution controls.

Cited by 9 publications

References 29 publications

Clinical staining of the ocular surface: Mechanisms and interpretations

Clinical staining of the ocular surface: Mechanisms and interpretations

Putting vital stains in context

Corneal, Limbal, and Conjunctival Epithelium of Bovine Eyes Imaged In Vitro by Using a Confocal Laser Scanning Microscope

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