Biocompatibility of hydrophilic silica-coated CdTe quantum dots and magnetic nanoparticles

Abstract

Fluorescent magnetic nanoparticles exhibit great application prospects in biomedical engineering. Herein, we reported the effects of hydrophilic silica-coated CdTe quantum dots and magnetic nanoparticles (FMNPs) on human embryonic kidney 293 (HEK293) cells and mice with the aim of investigating their biocompatibility. FMNPs with 150 nm in diameter were prepared, and characterized by high-resolution transmission electron microscopy and photoluminescence (PL) spectra and magnetometer. HEK293 cells were cultured with different doses of FMNPs (20, 50, and 100μ g/ml) for 1-4 days. Cell viability and adhesion ability were analyzed by CCK8 method and Western blotting. 30 mice were randomly divided into three groups, and were, respectively, injected via tail vein with 20, 60, and 100 μg FMNPs, and then were, respectively, raised for 1, 7, and 30 days, then their lifespan, important organs, and blood biochemical parameters were analyzed. Results show that the prepared water-soluble FMNPs had high fluorescent and magnetic properties, less than 50 μg/ml of FMNPs exhibited good biocompatibility to HEK293 cells, the cell viability, and adhesion ability were similar to the control HEK293 cells. FMNPs primarily accumulated in those organs such as lung, liver, and spleen. Lung exposed to FMNPs displayed a dose-dependent inflammatory response, blood biochemical parameters such as white blood cell count (WBC), alanine aminotransferase (ALT), and aspartate aminotransferase (AST), displayed significant increase when the FMNPs were injected into mice at dose of 100μg. In conclusion, FMNPs exhibit good biocompatibility to cells under the dose of less than 50 μg/ml, and to mice under the dose of less than 2mg/kg body weight. The FMNPs' biocompatibility must be considered when FMNPs are used for in vivo diagnosis and therapy.

HAI-178 antibody-conjugated fluorescent magnetic nanoparticles for targeted imaging and simultaneous therapy of gastric cancer

“…In our previous work, we prepared fluorescent magnetic nanoparticles (FMNPs) composed of silicon-wrapped magnetic nanoparticles and CdTe quantum dots and used FMNPs-labeled MSC cells to realize the targeted imaging and hyperthermia therapy of in vivo gastric cancer [33]. We also confirmed that the prepared fluorescent magnetic nanoparticles show good biocompatibility [34]. …”

A Comparative Study of CdTe Quantum Dots and CdTe@SiO2 Nanoparticles: Fabrication and Cytotoxicity in HEK293 Cells

“…25 Briefly, the six-well plates were coated with fibrinogen (5 g/mL) in Dulbecco Phosphate Buffered Saline (DPBS …”

“…The cells adhesion ability was performed by the method described by Ruan et al (2011). 25 Briefly, the six-well plates were coated with fibrinogen (5 g/mL) in Dulbecco Phosphate Buffered Saline (DPBS …”

Advances in the Toxicity of Nanomaterials

“…We also prepared hydrophilic high-luminescent magnetic nanocomposites (LMNCs), and investigated their influence on HEK293 cells and mice [47]. Results showed that the water soluble FMSNPs with dose less than 50 μg mL −1 exhibited well biosafety to HEK293 cells, FMSNPs with low dose (0.1 mg mL…”