Biocompatibility of NDGA-polymerized collagen fibers. I. Evaluation of cytotoxicity with tendon fibroblastsin vitro

Koob, Thomas J.; Willis, Toni A.; Hernández, Daniel J.

doi:10.1002/1097-4636(200107)56:1<31::aid-jbm1065>3.0.co;2-n

Cited by 46 publications

(40 citation statements)

References 24 publications

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“…Pure collagen scaffolds, on the other hand, do not possess elastomeric behavior and are generally weak at high porosity without chemical cross-linking (13). Cross-linking of collagen scaffolds is often performed to increase tensile strength and reduce enzymatic lability (18), but common chemical cross-linking techniques can reduce the cytocompatibility of the scaffold (12,14). Earlier reported composites of collagen with polymers involved collagen integration into hydrogel materials (1,4) and combinations with thermoplastic polymers that do not possess the elastic mechanical properties of polyurethanes (6).…”

Section: Discussionmentioning

confidence: 99%

Development of Composite Porous Scaffolds Based on Collagen and Biodegradable Poly(ester urethane)urea

2006

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Our objective in this work was to develop a flexible, biodegradable scaffold for cell transplantation that would incorporate a synthetic component for strength and flexibility and type I collagen for enzymatic lability and cytocompatibility. A biodegradable poly(ester urethane)urea was synthesized from poly(caprolactone), 1,4-diisocyanatobutane, and putrescine. Using a thermally induced phase separation process, porous scaffolds were created from a mixture containing this polyurethane and 0%, 10%, 20%, or 30% type I collagen. The resulting scaffolds were found to have open, interconnected pores (from 7 to >100 um) and porosities from 58% to 86% depending on the polyurethane/collagen ratio. The scaffolds were also flexible with breaking strains of 82-443% and tensile strengths of 0.97-4.11 MPa depending on preparation conditions. Scaffold degradation was significantly increased when collagenase was introduced into an incubating buffer in a manner that was dependent on the mass fraction of collagen present in the scaffold. Mass losses could be varied from 15% to 59% over 8 weeks. When culturing umbilical artery smooth muscle cells on these scaffolds higher cell numbers were observed over a 4-week culture period in scaffolds containing collagen. In summary, a strong and flexible scaffold system has been developed that can degrade by both hydrolysis and collagenase degradation pathways, as well as support cell growth. This scaffold possesses properties that would make it attractive for future use in soft tissue applications where such mechanical and biological features would be advantageous.

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Section: Discussionmentioning

confidence: 99%

Development of Composite Porous Scaffolds Based on Collagen and Biodegradable Poly(ester urethane)urea

2006

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“…The only two studies comparing the attachment of cells on two otherwise identical collagen surfaces that were either non-cross-linked or cross-linked were published by Koob et al, 22 who used the nordihydroguaiaretic acid (NDGA) technique, and by Kumar et al, 26 who used chitosan. The observation time points for the assessment of cell attachment were chosen as 24 to 48 h after cell seeding.…”

Section: Cell Attachment Morphology and Orientationmentioning

confidence: 99%

“…[13][14][15][16][17][18][19][20][21][22][23][24][25][26][27][28][29] However, it has also been shown that not all cell types show the same affi nity for a given surface. 28 The aim of the GTR procedure is the regeneration of a fully functional periodontium, including new cementum and periodontal ligament (PDL), as well as new bone.…”

Section: Cells Used For In Vitro Studiesmentioning

confidence: 99%

“…Of these, ten used human cells of either dermal (human dermal fi broblasts; HDF) or gingival (human gingival fi broblasts; HGF) origin. [17][18][19][20][21][23][24][25][26][27] Only Koop et al 22 used animal (bovine)-derived cells. Collagen sources, in contrast, were diverse, as were the modes of scaffold preparation and processing.…”

Section: Behavior Of Fi Broblasts On Collagen Membranesmentioning

confidence: 99%

“…This does not necessarily mean that cell proliferation can start only after this time period. Locci et al 20 and Marinucci et al 24 showed 3 H-thymidine incorporation into DNA, a sign of cell proliferation, after only 24 h. The effect of cross-linking on the speed or extent of cell attachment cannot be estimated, since only two studies 22,26 attempted such a comparison and only two cross-linking techniques were investigated. However, cross-linking in itself might not negatively affect cell attachment, whereas certain cross-linking techniques certainly do.…”

Section: Cell Attachment Morphology and Orientationmentioning

confidence: 99%

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Toward guided tissue and bone regeneration: morphology, attachment, proliferation, and migration of cells cultured on collagen barrier membranes. A systematic review

et al. 2008

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Collagen barrier membranes are frequently used in both guided tissue regeneration (GTR) and guided bone regeneration (GBR). Collagen used for these devices is available from different species and is often processed to alter the properties of the final product. This is necessary because unprocessed collagen is rapidly resorbed in vivo and demands for barrier membranes are different in GTR and GBR. This systematic literature review attempts to evaluate possible effects of collagen origin and mode of cross-linking on the potential of different cells to attach to, proliferate on, and migrate over barrier membranes in vitro. Seventeen original studies, selected by a systematic process, are included in this review. The results show that fibroblasts of different species and originating tissues as well as bone-forming cells are able to attach to collagen membranes irrespective of collagen origin or mode of processing. Different cell types behave differently on identical membranes. Many pieces of evidence are currently available, and we attempted to elucidate the effects of collagen origin and mode of processing on cellular behavior, but further research will be required before it will be possible to predict for certain the effect a specific procedure will have with a given product.

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Biocompatibility of NDGA-polymerized collagen fibers. II. Attachment, proliferation, and migration of tendon fibroblastsin vitro

Koob¹,

Willis²,

Qiu³

et al. 2001

J. Biomed. Mater. Res.

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The material properties of collagen fibers polymerized with nordihydroguaiaretic acid (NDGA) are equivalent to native tendon, suggesting that NDGA crosslinking may provide a viable approach to stabilizing collagenous materials for use in repairing ruptured, lacerated, or surgically transected fibrous tissues, such as tendons and ligaments (Koob & Hernandez, Biomaterials, in press). The present study evaluated the biocompatibility of these fibers with cultured bovine tendon fibroblasts. Fibroblast attachment, migration, and proliferation on NDGA-crosslinked materials were compared to those on prepolymerized type I tendon collagen constructs as well as on tissue-culture-treated plastic. Fibroblast attachment on NDGA-crosslinked collagen fibrils was equivalent to attachment on plates coated with collagen alone. Over a period of 8 days in culture, attached fibroblasts proliferated on NDGA-crosslinked collagen at a rate identical to that of fibroblasts attached to native collagen. In order for the biomaterial effectively to bridge gaps in fibrous tissues, fibroblasts must be able to migrate and replicate on the bridging fiber. Control and crosslinked fibers were inserted in calf tendon explants, with a portion of the fiber extending out of the sectioned end of the tendon. Explants were cultured for 9 weeks, and the number of cells was measured at weekly intervals. Cells appeared on the fibers after 1 week of culture. By 2 weeks, cells had colonized the entire fiber. The number of cells continued to increase throughout the 9 weeks in culture, forming a layer several cells thick. Histologic analysis indicated that the fibroblasts populating the fibers appeared to originate in the epitenon. There was no difference in the rate of fibroblast migration and replication, nor in the ultimate number of colonizing cells, between control collagen fibers and NDGA-crosslinked fibers. NDGA-crosslinked fibers may provide a means of bridging gaps in ruptured, lacerated, or surgically transected tendons by providing a mechanically competent scaffold on which tendon fibroblasts can migrate, attach, and proliferate.

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Biocompatibility of NDGA-polymerized collagen fibers. I. Evaluation of cytotoxicity with tendon fibroblastsin vitro

Cited by 46 publications

References 24 publications

Development of Composite Porous Scaffolds Based on Collagen and Biodegradable Poly(ester urethane)urea

Development of Composite Porous Scaffolds Based on Collagen and Biodegradable Poly(ester urethane)urea

Toward guided tissue and bone regeneration: morphology, attachment, proliferation, and migration of cells cultured on collagen barrier membranes. A systematic review

Biocompatibility of NDGA-polymerized collagen fibers. II. Attachment, proliferation, and migration of tendon fibroblastsin vitro

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