Biocompatibility of poly(2-alkyl-2-oxazoline) brush surfaces for adherent lung cell lines

Abstract

Development of synthetic surfaces that are highly reproducible and biocompatible for in vitro cell culture offers potential for development of improved models for studies of cellular physiology and pathology. They may also be useful in tissue engineering by removal of the need for biologically-derived components such as extracellular matrix proteins. We synthesised four types of 2-alkyl-2-oxazoline polymers ranging from the hydrophilic poly(2-methyl-2-oxazoline) to the hydrophobic poly(2-n-butyl-2-oxazoline). The polymers were terminated using amine-functionalised glass coverslips, enabling the synthetic procedure to be reproducible and scaleable. The polymer-coated glass slides were tested for biocompatibility using human epithelial (16HBE 14o-) and fibroblastic (MRC5) cell lines.Differences in adhesion and motility of the two cell types was observed, with the poly(2-isopropyl-2-oxazoline) polymer equally supporting the growth of both cell types, whereas poly(2-n-butyl-2-oxazoline) showed selectivity for fibroblast growth. In summary, 2-alkyl-2-oxazoline polymers may be a useful tool for building in vitro model cell culture models with preferential adhesion of specific cell types.

Poly(2‐oxazoline) Hydrogels: State‐of‐the‐Art and Emerging Applications

“…Some of this can be attributed to the different cell types being used and cell lines versus primary cells, and some of it to the type of PAOx and the preparation method. For instance Davies and co‐workers exploited the living nature of PAOx synthesis to conjugate PMeOx, PEtOx, poly(2‐isopropyl‐2‐oxazoline) (PiPropOx), or poly(2‐butyl‐2‐oxazoline) (PButylOx) to amine‐functional glass slides by using aminofunctionalized glass slides as the terminating group for the CROP. Regardless of the type of polymer used, both epithelial and fibroblasts cell lines were able to grow to confluency.…”

The Design of Sulfobetaine Polymers with Thermoresponsiveness under Physiological Salt Conditions

“…PNIPAMs have been developed for application in in vitro biomaterials including cell culture dishes,4 cell separation,5 intracellular temperature probes,6 etc. Poly(2‐isopropyl‐2‐oxazoline)s7 and elastin base peptides8 have also been investigated for use as biomaterials and show superior biocompatibility compared to PNIPAM. Compared to LCST polymers, UCST polymers have not been studied as much.…”

One-pot synthesis of amphiphilic multiblock poly(2-oxazoline)s via para-fluoro-thiol click reactions

“…These vials were then transferred to the microwave reactor, heated to 140°C, and quenched with a sodium hydroxide/methanol solution at predetermined time points. 1 H NMR and SEC analysis were conducted to determine the monomer conversion, the molar mass of obtained polymers at different time points as well as the polymerization rate constant (Fig. 1).…”

“…A significant shift of polymer peaks indicating the coupling reaction was happening and generated tetrablock polymers, although the low molecular weight tailing in the SEC trace of P8 suggesting the existence of unreacted diblock polymers. 1 H NMR and MALDI-ToF characterization results of the coupling reaction of PFB-PEtOx with dithiol A is displayed in the ESI (Fig. S3 †).…”

“…Poly(2-oxazoline)s(POx) have attracted great attention as they possess various properties including biocompatibility, [1][2][3][4][5][6][7][8] thermal responsive ability, [9][10][11][12] tunable hydrophilicity 9,13 and adjustable cytotoxicity 7,14,15 that are useful for biomedical and industrial applications such as adhesives 10 and lubricants. 16 Synthesis of poly(2-oxazoline)s with well-defined chain length, and end groups has been achieved through a living cationic ring-opening polymerization (CROP) process that can be initiated by various commercially available initiators such as tosylates, triflates, acid halides, and benzyl halides.…”