Biocompatibility of printed paper-based arrays for 2-D cell cultures

A paper-based scaffold for enhanced osteogenic differentiation of equine adipose-derived stem cells

“…Data are shown as mean ± SD (n = 3). *p \ 0.05 effect of substrate surface factors on the attachment and growth of cells (Juvonen et al 2013). Additionally, paper has been impregnated with cell-containing hydrogels and stacked together to create multilayer constructs for 3D cell culture, which allow for controlled cellular distribution within the porous 3D structure, as well as separation of the individual layers for subsequent analysis (Deiss et al 2013;Derda et al 2009Derda et al , 2011.…”

Printing technologies for biomolecule and cell-based applications

“…There is also a small pressure between 50 and 150 N applied when transferring ink to the substrate. Application of flexographic printing for functional materials has mostly concentrated on printed electronics (Tobjörk and Österbacka, 2011) and only few examples exists about the usage of flexographic printing in biological applications, most concerning paper-based diagnostics (Juvonen et al, 2013;Määttänen et al, 2011Määttänen et al, , 2014Olkkonen et al, 2010;Phillips et al, 2012). For example, Phillips et al have shown that pattern arrays of antibodies can be successfully transferred on flexible plastic films and the proteins retain their activity (Phillips et al, 2012).…”

“…Fig. 2 shows a schematic presentation of the application of roll-to-roll fabricated printed paperbased 2D reaction array platform that can be used e.g., for screening assays and for which dispensing can for instance be made with inkjet printing technology (Juvonen et al, 2013).…”

Printing technologies in fabrication of drug delivery systems

“…Apart from being used in graphical printing, flexo printing has also been used for printed electronics applications to fabricate conducting structures and electrical components from metal, carbon and conductive polymer-based inks [25][26][27][28][29][30][31][32][33]. In addition, flexo printing has been utilized in printed diagnostics for the preparation of paper-based reaction arrays for cell assays, enzyme-based indicators and lateral flow structures [34,35].…”