Biocompatibility of retrograde filling materials in the ferret canine Amalgam and IRM

Maher, William P.; Johnson, Richard L.; Hess, John A.; Steiman, Harvey

doi:10.1016/0030-4220(92)90021-h

Cited by 37 publications

(22 citation statements)

References 9 publications

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“…17 These results reflected the type of biomaterial connective tissue interface obtained with these materials in vivo where a fibrous encapsulation or chronic inflammatory response rather than the formation of a periodontal connective tissue attachment has been reported. [22][23][24] However, the optimal result after periodontal wound healing is the regeneration of a periodontal connective tissue attachment. Therefore, the objective of the present study was to determine whether the application of EMD, a preparation thought to be inductive of cementum formation, would alter the phenotype of PDL cells when cultured on materials that have not been reported to support periodontal connective tissue attachment in vivo.…”

Section: Discussionmentioning

confidence: 99%

Effect of enamel matrix proteins on the phenotype expression of periodontal ligament cells cultured on dental materials

Inoue

LeGeros

Hoffman

et al. 2004

J Biomedical Materials Res

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Cells within the periodontal ligament have the potential to regenerate a periodontal connective tissue attachment on pathologically exposed root surfaces as well as on several material surfaces including titanium. However, rather than a periodontal connective tissue attachment, a fibrous encapsulation or chronic inflammatory response has been reported at the material connective tissue interface for most dental materials. Cementum is the first tissue of the periodontal connective tissue attachment to develop and the secretion of enamel matrix related proteins on the newly mineralized dentin surface precedes and is thought to induce cementum formation. Enamel matrix-related proteins may also function in the adult because the application of an acid extract of porcine enamel protein matrix (Emdogain(R), EMD) on pathologically exposed root surfaces has been shown to result in cementum regeneration. Therefore, the objective of the present study was to determine whether the application of EMD to materials that do not normally support cementogenesis in vivo would alter the in vitro phenotype of periodontal ligament (PDL) cells including the synthesis of cementum-associated extracellular matrix proteins. Primary PDL cells were established from 21-day-old Sprague-Dawley rats, and were cultured on four materials commonly encountered in dental practice (gutta percha, calcium hydroxide, amalgam, and super EBA cement) with and without the application of EMD. After 7 or 14 days of culture, total-DNA content, collagen synthesis, alkaline phosphatase activity, and the synthesis of a 42-kDa cementum-associated extracellular matrix protein were determined. PDL cells cultured on all materials had decreased total DNA content. The application of EMD further decreased total DNA content. PDL cells cultured on gutta percha and calcium hydroxide with the application of EMD had similar levels of collagen synthesis and alkaline phosphatase activity but also expressed a 42-kDa cementum extracellular matrix-associated protein when compared to the other groups. These results suggest that EMD can alter the phenotype of PDL cells when cultured on these dental materials.

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Section: Discussionmentioning

confidence: 99%

Effect of enamel matrix proteins on the phenotype expression of periodontal ligament cells cultured on dental materials

Inoue

LeGeros

Hoffman

et al. 2004

J Biomedical Materials Res

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“…20 Primary periodontal ligament cells similarly cultured on amalgam, calcium hydroxide, or gutta percha, did not express the cementum-associated extracellular matrix protein, 21 results that reflect the response to these materials in vivo. 6,7 However, in a companion article, we report that EMD applied to gutta percha or calcium hydroxide permitted the expression of the cementum extracellular matrix protein by primary periodontal ligament cells after 7 days of culture. 22 These results suggest that the application of EMD can alter the phenotype of periodontal ligament cells when cultured on these materials.…”

Section: Introductionmentioning

confidence: 95%

“…Rather, a fibrous encapsulation or a chronic inflammatory response has been reported at the periodontal connective tissue material interface with these materials. 6,7 Because the periodontal ligament contains pluripotental cell populations, it may be possible to increase the clinical success rate of procedures using these materials if one were able to induce…”

Section: Introductionmentioning

confidence: 99%

Effect of enamel matrix proteins on the periodontal connective tissue‐material interface after wound healing

Craig

Kallur

Inoue

et al. 2004

J Biomedical Materials Res

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The periodontal ligament has the potential to regenerate a complete periodontal connective tissue attachment, starting with the deposition of cementum, on pathologically exposed root surfaces as well as several materials including titanium oxide. However, most commonly used dental materials result in a fibrous encapsulation or a chronic inflammatory response after periodontal wound healing rather than the formation of a periodontal connective tissue attachment. Recently, an extract of porcine enamel matrix (Emdogain(R), EMD) has been reported inductive of cementum formation in both in vivo and in vitro studies. The aim of this study was to determine the effect of EMD, when applied to materials previously reported not supportive of periodontal connective tissue formation, on the periodontal connective tissue-material interface obtained with these materials in vivo. Bilateral osteotomies were performed on the mandible of a Yucatan minipig exposing the buccal root surface of four premolars. A series of four preparations were placed in each root surface that were subsequently filled with calcium hydroxide, gutta percha, mineral trioxide aggregate (MTA), or left unfilled. One side, in addition, received an application of EMD prior to surgical closure. A bioabsorbable surgical barrier membrane was placed over the osteotomy sites to exclude gingival connective tissue from the wound-healing environment. The mucoperiosteal flaps were then readapted and sutured in position. The animal was euthanized 10 weeks after the procedure, block sections obtained and prepared for light microscopy. Results demonstrated complete regeneration of alveolar bone and periodontal ligament in all four teeth from the EMD-treated side. Fibers from the periodontal ligament were observed to insert into a mineralized matrix consistent with cementum on all four root preparations. In contrast, massive root resorption without regeneration of alveolar bone was found on all teeth from the side not treated with EMD. The results of this pilot study suggest that the application of EMD to material surfaces that normally do not support periodontal connective tissue attachment formation can alter the type of periodontal connective tissue interface obtained with these materials.

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“…However, Data were expressed in number; Statistical analysis was done by Chi-square test was performed to compare between groups; n = Number of subject incidence of pain by MTA or IRM may be due inadequacy of the repair, 15 or extrusion of the material into the furcal area. 2 Furthermore, it is reported that IRM causes persistent inflammation and slower healing potential, 16 and it is more cytotoxic than the MTA. 17 It might be the possible reason for higher incidence of pain in IRM treated teeth.…”

Section: Discussionmentioning

confidence: 99%

Clinical and radiological evaluation of furcal perforation repaired by mineral trioxide aggregate and intermediate restorative material

Shomi

Hossain

Alam

2017

Bangabandhu Sheikh Mujib Medical University Journal

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<p class="Abstract">The purpose of the present study was to assess the clinical and radiological outcome following repair of furcal perforation by mineral trioxide aggregate (MTA) and intermediate restorative material (IRM) in mandibular molar teeth. Forty teeth having furcal perforation were enrolled in this study, out of which 20 teeth were treated with MTA and the remaining 20 teeth were subjected to IRM treatment. Following perforation repair, all teeth were subjected to root canal treatment followed by final restoration. Clinical and radiological outcome was evaluated at 3, 6 and 12 months interval. The results showed that in both MTA and IRM groups, pain, tenderness on percussion as well as swelling and sinus was gradually decreased with the increase of the observation period. Furthermore, the widening of the periodontal ligament space and communi-cation with the oral cavity were gradually decreased. Although there was no significant differences between MTA and IRM at 3 and 6 months observation period but at 12 months, the clinical outcome between MTA and IRM was statistically significant (p<0.05). It can be concluded that repair of furcal perforation by MTA showed more effective than that of IRM.</p>

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Biocompatibility of retrograde filling materials in the ferret canine Amalgam and IRM

Cited by 37 publications

References 9 publications

Effect of enamel matrix proteins on the phenotype expression of periodontal ligament cells cultured on dental materials

Effect of enamel matrix proteins on the phenotype expression of periodontal ligament cells cultured on dental materials

Effect of enamel matrix proteins on the periodontal connective tissue‐material interface after wound healing

Clinical and radiological evaluation of furcal perforation repaired by mineral trioxide aggregate and intermediate restorative material

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