Biocompatibility of Ricinus communis polymer with addition of calcium carbonate compared to titanium. Experimental study in guinea pigs

Abstract

ObjectiveThe aim of the present investigation was to determine whether the difference in inflammatory tissue reaction between the Riccinus communis (castor) polymer with calcium carbonate and the titanium implant is statistically significant.MethodsThirty-two Cavia porcellus were allocated into four groups of eight animals each. We implanted the two types of materials in the retroperitoneal space of all the animals. They were euthanized at 7, 20, 30 and 40 days after surgery, and an histological study of the samples was conducted.ResultsAll implants showed characteristics of chronic inflammation regardless of the material and timepoint of evaluation. There was no statistically significant difference between Pm+CaCO3 and Ti with regard to the presence of granulation tissue, tissue congestion, histiocytes, lymphocytes, neutrophils, giant cells, and fibrosis (P> 0.05).ConclusionThe castor oil polymer plus calcium carbonate implant was not statistically different from the titanium implant regarding inflammatory tissue reaction.

Polyurethane derived from Ricinus Communis as graft for bone defect treatments

“…They found that the calcium phosphate composite improves biocompatibility and osseointegration speed. In another experimental study on the biocompatibility of the Ricinus communis polymer with addition of calcium carbonate in comparison to titanium, a recognized biocompatible material, it was evidenced that composite was not statistically different from the titanium implant regarding tissue inflammatory reaction [20] . Another biomaterial used in this work is the latex derived from the rubber tree Hevea brasilienses.…”

Semiautomated quantification of the fibrous tissue response to complex three‐dimensional filamentous scaffolds using digital image analysis

“…Histochemical staining methods as well as immunohistochemical staining methods are described in the literature 55,62‐64 . The histochemical staining methods mainly concentrated on Picro‐Sirius Red and Masson's trichrome staining methods 14,29,31‐38,40,41,43‐47,65‐70 . In this context, Picro‐Sirius Red staining was mostly performed to evaluate organ fibrosis while Masson's trichrome stainings were commonly used to measure biomaterial‐induced fibrosis.…”

“…Sections were stained with Masson–Goldner's trichrome staining, to differentiate collagen fibers from the surrounding tissue. Masson–Goldner's trichrome staining is widely used for the analysis of fibrosis in organs and for the evaluation of the fibrotic response to biomaterials 14,35,38,40‐47 . As a result of this procedure, collagen fibers appear in a bright green color in bright field light microscopy, which produces excellent contrast for analysis with digital image quantification.…”

“…This infiltrating fibrotic growth is not adequately represented by the commonly used analytical tools. Still semiquantitative assessments remain in use to measure this fibrous tissue response on biomaterials 10‐18 . Although practical to use, these methods show significant disadvantages: they are in a way unspecific, subjective, lab‐to‐lab variable and not uniformly applicable to the diversity of existing biomaterial designs 4,5,19 .…”

Castor polyurethane used as osteosynthesis plates: microstructural and thermal analysis

“…Castor oil presents in its composition 89.5% to 90% triglyceride ricinoleic acid (12-hydroxy-cis-9-octadecenoic acid), whose molecular formula is C 17 H 32 OHCOOH. In the ester bonds of the triglyceride (1st carbon) reactions of hydrolysis, esterification, alcoholysis and, halogenation can occur (Figure 1) [9][10][11][12] .…”