Biocompatibility of vertically aligned multi-walled carbon nanotube scaffolds for human breast cancer cell line MDA-MB-231

Abstract

The aim of the current study was to determine whether the MWCNT-based scaffold has a suitable structure for cell growth and provides a biocompatible environment for human MDA-MB-231 cell lines. MWCNT-based nanostructured scaffolds were produced by plasma-enhanced chemical vapor deposition (PECVD) technique. MDA-MB-231 cells were seeded on MWCNTs-textured silicon scaffolds and on pristine silicon surfaces. After 1 week of culturing, the scaffolds were prepared for SEM analysis and immunocytochemical staining was performed for the two groups (MWCNT scaffold and pristine silicon surface), using MMP-2, MMP-9, PI3K, AKT and NF-κB primary antibodies. SEM analyses showed that the MDA-MB-231 cells better adhered to the MWCNT-based nanostructured scaffold than the pristine silicon surface. Immunohistochemical activity of the MDA-MB-231 cells on both materials has similar staining with anti-AKT MMP-2, MMP-9 and NF-κB primary antibodies. Therefore, the results of the present study suggest that the MWCNT-based scaffolds enhanced cell adhesion to the scaffold and exhibited more biomimetic properties and physiological adaptation with the potential to be used for in vitro metastasis studies of BrCa cell lines.

Nanomedicine-Based Delivery Strategies for Breast Cancer Treatment and Management

“…By using the plasma-enhanced chemical by vapor deposition (PECVD) process, Akinoglu, E. M. et al [ 113 ] determined that a multi-walled carbon nanotube-based scaffold has a good shape for cell development and offers a biocompatible environment for human MDA-MB-231 cell lines. The current findings revealed improved cell adherence to the scaffold and displayed excellent biomimetic features and physiological adaptability, suggesting that they may be utilized to research BrCa cell line metastasis in vitro.…”

<p>Past, Present, and Future of Anticancer Nanomedicine</p>

“… 267 , 276 - 278 Some carbon nanotube based materials also have shown high light-to-heat conversion efficiency and biocompatibility. 279 , 280 In addition, light-absorbing organic NPs, such as polyaniline conductive polymers and indocyanine green (ICG) are also attractive materials as photothermal agents since those materials are biodegradable unlike other inorganic NPs. 281 – 285…”

“…267,[276][277][278] Some carbon nanotube based materials also have shown high light-to-heat conversion efficiency and biocompatibility. 279,280 In addition, light-absorbing organic NPs, such as polyaniline conductive polymers and indocyanine green (ICG) are also attractive materials as photothermal agents since those materials are biodegradable unlike other inorganic NPs. [281][282][283][284][285] The combined use of NPs and PTT has shown therapeutic efficacy for untreated and distant CT26 colon carcinoma and metastasized TC-1 submucosa-lung cancer, which were originally inaccessible by conventional PTT.…”

Radiotherapy and Immmun Responce Is the Radiotherapy Vaccine? Good News?

“…In this review will be discussed the role of radiotherapy for immun response. As seen on the Figure 1 the human breast cancer cell line MDA-MB-231 are growing easy on biocompatibility of vertically aligned multi-walled carbon nanotube scaffolds [1]. There is smilar cancer growth in human body and our immun system is trying to inhibit the cancer cells.…”