Biocompatible Abscisic Acid-Sensing Supramolecular Hybridization Probe for Spatiotemporal Fluorescence Imaging in Plant Tissues

Wu, Meng; Yin, Chenhui; Jiang, Xinxin; Sun, Qijun; Xu, Xiaoyu; Ma, Yinghe; Liu, Xinjian; Niu, Na; Chen, Ligang

doi:10.1021/acs.analchem.2c01050

Cited by 22 publications

(5 citation statements)

References 30 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Wu et al (2022) labeled an AIEgens fluorescent probe to abscisic acid (ABA) in the presence of bovine serum albumin (BSA). This method provided a novel perspective for in vivo or in vitro ABA detection [ 88 ].…”

Section: Application Of Aiegens As Fluorescent Probes and Possibiliti...mentioning

confidence: 99%

Aggregation-Induced Emission Luminogens: A New Possibility for Efficient Visualization of RNA in Plants

Yang,

Zhao,

Sang

et al. 2024

Plants

View full text Add to dashboard Cite

RNAs play important roles in regulating biological growth and development. Advancements in RNA-imaging techniques are expanding our understanding of their function. Several common RNA-labeling methods in plants have pros and cons. Simultaneously, plants’ spontaneously fluorescent substances interfere with the effectiveness of RNA bioimaging. New technologies need to be introduced into plant RNA luminescence. Aggregation-induced emission luminogens (AIEgens), due to their luminescent properties, tunable molecular size, high fluorescence intensity, good photostability, and low cell toxicity, have been widely applied in the animal and medical fields. The application of this technology in plants is still at an early stage. The development of AIEgens provides more options for RNA labeling. Click chemistry provides ideas for modifying AIEgens into RNA molecules. The CRISPR/Cas13a-mediated targeting system provides a guarantee of precise RNA modification. The liquid–liquid phase separation in plant cells creates conditions for the enrichment and luminescence of AIEgens. The only thing that needs to be looked for is a specific enzyme that uses AIEgens as a substrate and modifies AIEgens onto target RNA via a click chemical reaction. With the development and progress of artificial intelligence and synthetic biology, it may soon be possible to artificially synthesize or discover such an enzyme.

show abstract

Section: Application Of Aiegens As Fluorescent Probes and Possibiliti...mentioning

confidence: 99%

Aggregation-Induced Emission Luminogens: A New Possibility for Efficient Visualization of RNA in Plants

Yang,

Zhao,

Sang

et al. 2024

Plants

View full text Add to dashboard Cite

show abstract

“…Lu et al used Kaempferrol as an AIE fluorescent probe to detect Al3+ in Arabidopsis thaliana roots incubated in a mixture of water and tetrahydrofuran containing Kaempferrol due to the plant's high metal content [81]. Plant hormones as an essential plant growth regulator, Wu et al discovered an Abscisic acid (ABA) AIEgens fluorescent probe that can label ABA in the presence of bovine serum albumin (BSA), providing a novel perspective on in vivo or in vitro ABA detection [82].…”

Section: Aiegens Enhance the Efficiency Of Photosynthesis In Plantsmentioning

confidence: 99%

Aggregation-Induced Emission Luminogens (AIEgens):A New Possibility for Efficient Visualization of RNA in Plants

Yang,

Zhao,

Sang

et al. 2023

Preprint

View full text Add to dashboard Cite

RNA, as an important substance for regulating biological growth and development, has significant implications for visualization research. However, many spontaneously fluorescent substances in plants greatly interfere with the effectiveness of plant bioimaging. Aggregetion- induced emission luminogens (AIEgens), due to their luminescent properties, tunable molecular size, high fluorescence intensity, good photostability, and low cell toxicity, have been widely applied in the animal and medical fields. We have found that AIEgens have great potential as RNA fluorescent probes for efficient imaging in plants. In this review, we first introduce several common RNA labeling forms and point out their pros and cons. We briefly describe the development of AIEgens and the AIE mechanism, and then present various practical applications of AIEgens, including detailed examples of their use as biological markers. To further promote the application of AIE in the field of RNA, we suggest the use of AIEgens to modify target RNA via techniques such as click chemistry or CRISPR/Cas, to achieve RNA visualization in plants. highly possible to modify target RNA with AIEgens in vivo for RNA visualization.

show abstract

“…Fluorescence imaging is a noninvasive method, − which is conducive to detecting and monitoring the variation of in situ biomarkers in a real-time manner in plants. − As for in vivo fluorescence imaging in plants, the imaging quality in the shorter wavelength range (e.g., visible light (400–700 nm) or NIR-I range (700–900 nm)) is usually compromised or eroded because such pigments as chlorophylls, anthocyanins, and carotenoids in plants usually have absorptions in the visible-light wavelength range and exhibit fluorescence in the visible-light or NIR-I wavelength range. − However, near-infrared second window (NIR-II, emission: 900–1700 nm) fluorescence imaging can achieve much superior imaging performance, − owing to no interference from plant-related pigments in the NIR-II wavelength range.…”

Section: Introductionmentioning

confidence: 99%

Water-Dispersible Activatable Nanoprobe for Detecting Cadmium-Ion-Induced Oxidative Stress in Edible Crops via Near-Infrared Second-Window Fluorescence Imaging

et al. 2022

View full text Add to dashboard Cite

Edible crops are important in terms of food security and sustainable agriculture. Heavy-metal-ion contamination of water/soil has deleterious impacts on the growth of edible crops. Among the heavy metals, cadmium (Cd) is toxic to plants, people, and animals, as it is widely used in industry; it has become the most important metal ion in the soil/water pollution. Once the toxic Cd ion enters edible crops via the water/soil in which the crops grow, it will induce oxidative stress (overproduction of reactive oxygen species with H2O2 being the most abundant) in the crops, and strong oxidative stress leads to the crops’ growth depression or inhibition. Hence, it is of great significance to accurately monitor the oxidative stress induced by Cd ions in edible crops, as the monitoring results could be employed for the early warning of Cd-ion pollution in water/soil. Herein, we design an activatable nanoprobe that can detect Cd-ion-induced oxidative stress in edible crops via near-infrared second-window (NIR-II) fluorescence imaging. The molecular probe IXD-B contains the diphenylamine-modified xanthene group acting as the electron-donating unit, bis(methylenemalononitrile)indan as the electron-accepting unit, and the methenephenylboronic acid group as the recognition moiety for H2O2 and the fluorescence quencher. The probe molecules being encapsulated by the amphiphilic DSPE-PEG2000 render the water-dispersible nanoprobe (IXD-B@DSPE-PEG2000). When the nanoprobe enters the edible crops, it can be activated by the overexpressed H2O2 therein and consequently emit strong NIR-II fluorescence signals for visualizing and tracking the oxidative stress in edible crops induced by Cd ions.

show abstract

Biocompatible Abscisic Acid-Sensing Supramolecular Hybridization Probe for Spatiotemporal Fluorescence Imaging in Plant Tissues

Cited by 22 publications

References 30 publications

Aggregation-Induced Emission Luminogens: A New Possibility for Efficient Visualization of RNA in Plants

Aggregation-Induced Emission Luminogens: A New Possibility for Efficient Visualization of RNA in Plants

Aggregation-Induced Emission Luminogens (AIEgens):A New Possibility for Efficient Visualization of RNA in Plants

Water-Dispersible Activatable Nanoprobe for Detecting Cadmium-Ion-Induced Oxidative Stress in Edible Crops via Near-Infrared Second-Window Fluorescence Imaging

Contact Info

Product

Resources

About