Biocompatible electrospinning chitosan nanofibers: A novel delivery system with superior local cancer therapy

Sedghi, Roya; Shaabani, Alireza; Mohammadi, Zohreh; Samadi, Fatemeh; Isaei, Elham

doi:10.1016/j.carbpol.2016.12.011

Cited by 121 publications

(40 citation statements)

References 49 publications

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“…In addition, the carbopol/PCL diameter (with 30:70 ratio) in our previous study was reported to be 1.25 ± 0.43 μm for the first time, which is similar to current results . According to Sedghi et al and Golchin et al, incorporation of Cur into CS/PVA nanofibers decreases nanofiber diameter, as confirmed by our results. FTIR measurement also confirmed the presence of the Cur and all other polymers in the fabricated scaffolds.…”

Section: Discussionsupporting

confidence: 92%

Wound healing improvement by curcumin‐loaded electrospun nanofibers and BFP‐MSCs as a bioactive dressing

Golchin

Hosseinzadeh

Jouybar

et al. 2020

Polymers for Advanced Techs

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Wound healing, one of the most complex processes of the body involving the cooperation of several important biomolecules and pathways, is one of the major therapeutic and economic issues in regenerative medicine. The present study aimed to introduce a novel electrospun curcumin (Cur)-incorporated chitosan/polyvinyl alcohol/carbopol/polycaprolactone nanofibrous composite for concurrent delivery of the buccal fat pad-derived mesenchymal stem cells (BFP-MSCs) and Cur to a fullthickness wound on the mouse model. Scaffolds were characterized structurally using scanning electron microscopy (SEM), fluorescence microscopy imaging and Fouriertransform infrared spectroscopy, and toxicity of the scaffolds was also evaluated after BFP-MSC seeding by SEM imaging and 3-(4,5 dimethyiazol-2-1)-2-5-diphenyl tetrazolium bromide (MTT) assay. Then, its influence on the wound-healing process was investigated as a wound dressing for a full-thickness skin defect in mouse model.Results demonstrated that the designed composite scaffolds have the capability for cell seeding and support their growth and proliferation. Macroscopic and histopathological characteristics were evaluated at the end of the 7 and 14 days after surgery, and their results showed that our designed scaffold groups accelerated the woundhealing process compared with the control group. Among those, scaffold/Cur, scaffold/Cur/BFP-MSC and scaffold/BFP-MSC groups demonstrated more wound repair efficacy. These results indicated that the combined grafts can be used to improve the wound-healing process, and therefore, the electrospun nanofibers presented in this study, Cur and BFP-MSC together, were demonstrated to have promising potential for wound-dressing applications. K E Y W O R D S chitosan/polyvinyl alcohol/carbopol/polycaprolactone, curcumin, electrospun nanofibers, mesenchymal stem cells, regenerative medicine, wound healing

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Section: Discussionsupporting

confidence: 92%

Wound healing improvement by curcumin‐loaded electrospun nanofibers and BFP‐MSCs as a bioactive dressing

Golchin

Hosseinzadeh

Jouybar

et al. 2020

Polymers for Advanced Techs

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“…35,36 It is also reported that Cur loading affects the fiber diameter and morphology when Cur is added to spinning solution by decreasing of the average diameter of the nanofibers. 24,35 Bead formation, fiber diameter adjustment, fibers deformity, burst Cur releases, and other restrictions on the mechanical properties of the fibrous composites motivates scientists for study to achieve an ideal Cur nanofibrous incorporated scaffold. 36,37 In the current work, Cur/CS/PVA+carbopol/PCL, and CS/PVA+carbopol/PCL electrospun scaffold were prepared and characterized and then the capability of the engineered fibrous composite for Cur and BFP-MSC delivery was examined simultaneously.…”

Section: Discussionmentioning

confidence: 99%

Biological behavior of the curcumin incorporated chitosan/poly(vinyl alcohol) nanofibers for biomedical applications

Golchin

Hosseinzadeh

Staji

et al. 2019

J of Cellular Biochemistry

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Electrospun composite scaffolds show high ability to be used in regenerative medicine and drug delivery, due to the nanofibrous structure and high surface area to volume ratio. In this study, we used nanofibrous scaffolds fabricated by chitosan (CS), poly(vinyl alcohol) (PVA), carbopol, and polycaprolactone using a dual electrospinning technique while curcumin (Cur) incorporated inside of the CS/PVA fibers. Scaffolds were fully characterized via scanning electron microscopy, water contact angle, tensile measurement, hydration, protein adsorption, and wrinkled tests. Furthermore, viability of the buccal fat pad‐derived mesenchymal stem cells (BFP‐MSCs) was also investigated using MTT assay for up to 14 days while cultured on these scaffolds. Cell cycle assay was also performed to more detailed evaluation of the stem cells growth when grown on scaffolds (with and without Cur) compared with the culture plate. Results demonstrated that Cur loaded nanofibrous scaffold had more suitable capability for water absorption and mechanical properties compared with the scaffold without Cur and it could also support the stem cells viability and proliferation. Cur release profile showed a decreasing effect on BFP‐MSCs viability in the initial stage, but it showed a positive effect on stem cell viability in a long‐term manner. In general, the results indicated that this nanofibrous scaffold has great potential as a delivery of the Cur and BFP‐MSCs simultaneously, and so holds the promising potential for use in various regenerative medicine applications.

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“…To achieve enough initial dosage to kill tumours, the burst release of anti-neoplastic drugs is necessary. And for those that may survive from the initial burst, a continued release is also essential to prevent their further proliferation and migration [ 21 ]. In addition, we can see that the cumulative release of PPD from PCL/3%PPD, PCL/5%PPD, PCL/10%PPD and PCL/20%PPD nanofibres in 144 h was 29.53%, 34.06%, 38.51% and 50.07%, respectively, which was increased as the drug-loaded concentration increased.…”

Section: Resultsmentioning

confidence: 99%

Polycaprolactone nanofibres loaded with 20( S )-protopanaxadiol for in vitro and in vivo anti-tumour activity study

et al. 2018

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In this work, 20(S)-protopanaxadiol (PPD)-loaded polycaprolactone (PCL) nanofibres were successfully fabricated by the electrospinning technique using Tween 80 as a solubilizer. Firstly, smooth and continuous nanofibres were collected using suitable solvents and appropriate spinning conditions. Secondly, nanofibre mats were characterized by scanning electron microscopy, thermogravimetric (TG) analysis, Fourier transform infrared spectroscopy and mechanical testing. Finally, nanofibrous membranes were evaluated using water contact angle, in vitro drug release, biodegradation test, in vitro and in vivo anti-tumour activity and cell apoptosis assay. Scanning electron microscopic observations indicated that the diameter of the drug-loaded nanofibres increased with the increase of drug concentration. TG analysis and mechanical test showed that nanofibres were equipped with great thermal and mechanical properties. Biodegradation test exhibited that the structure of fabricated nanofibres had a certain degree of change after 15 days. An in vitro release study showed that PPD from drug-loaded nanofibres could be released in a sustained and prolonged mode. The cytotoxic effect of drug-loaded nanofibre mats examined on human laryngeal carcinoma cells (Hep-2 cells) demonstrated that the prepared nanofibres had a remarkable anti-tumour effect. Meanwhile, the drug-loaded fibre mats showed a super anti-tumour effect in an in vivo anti-tumour study. All in all, PCL nanofibres could be a potential carrier of PPD for cancer treatment.

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Biocompatible electrospinning chitosan nanofibers: A novel delivery system with superior local cancer therapy

Cited by 121 publications

References 49 publications

Wound healing improvement by curcumin‐loaded electrospun nanofibers and BFP‐MSCs as a bioactive dressing

Wound healing improvement by curcumin‐loaded electrospun nanofibers and BFP‐MSCs as a bioactive dressing

Biological behavior of the curcumin incorporated chitosan/poly(vinyl alcohol) nanofibers for biomedical applications

Polycaprolactone nanofibres loaded with 20( S )-protopanaxadiol for in vitro and in vivo anti-tumour activity study

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