Biocompatible fluorescent carbon quantum dots prepared from beetroot extract for <i>in vivo</i> live imaging in <i>C. elegans</i> and BALB/c mice

Singh, Vikram; Rawat, Kundan Singh; Mishra, Shachi; Baghel, Tanvi; Fatima, Sana; John, Aijaz Ahmad; Kalleti, Navodayam; Singh, Divya; Nazir, Aamir; Rath, Srikanta Kumar; Goel, Atul

doi:10.1039/c8tb00503f

Cited by 107 publications

(62 citation statements)

References 33 publications

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“…In vitro studies demonstrate rapid intracellular uptake and do not show significant toxicity even at extremely high concentrations [29]. In vivo and ex vivo imaging in BALB/c mice show similar results with no observable toxicity and rapid clearance from the reticuloendothelial system [30]. Furthermore, CDs have previously demonstrated comparable two-photon cross-sections to those of commercially-available quantum dots, making them highly valuable as probes for bioimaging applications [31].…”

Section: Introductionmentioning

confidence: 99%

Carbon dot-protoporphyrin IX conjugates for improved drug delivery and bioimaging

2019

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Photodynamic therapy (PDT) uses photosensitisers such as protoporphyrin IX (PpIX) to target tumours via the release of toxic singlet oxygen when irradiated. The effectivity of the treatment is limited by the innate properties of the photosensitizers; they typically exhibit inefficient accumulation in target tissue and high dark toxicity. Carbon dots (CDs) are biocompatible fluorescent nanoparticles which can improve PpIX cellular uptake and solubility. In this work, we present conjugates synthesised by host-guest encapsulation (PpIX@CD) and amide cross-linking (PpIX-CD). Characterization demonstrated conjugates have a loading efficiency of 34–48% and similar singlet oxygen production to PpIX. PpIX-containing CDs showed a 2.2 to 3.7-fold decrease in dark toxicity. PpIX-CD and PpIX@CD showed equivalent light-induced toxicity to PpIX in concentrations >1 μg/ml, leading to a 3.2 to 4.1-fold increase in photo-toxicity index (PI). The less soluble fraction of cross-linked conjugates (PpIX-CD)p did not show significant difference from PpIX. Confocal light scanning microscopy demonstrated rapid intracellular uptake and accumulation of conjugates. Our results demonstrate the variations between cross-linking strategies in CD-based conjugates, highlighting their potential as carriers in drug delivery and bioimaging applications.

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Section: Introductionmentioning

confidence: 99%

Carbon dot-protoporphyrin IX conjugates for improved drug delivery and bioimaging

2019

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“…The excitation-dependent emission behavior is one of the typical photophysical characteristics of CQDs that originates from the surface state of these nanomaterials. For instance, Goel and co-workers prepared two fluorescent CQDs with blue and green emissions using hydrothermal and chemical oxidation methodologies, respectively (Singh et al, 2018 ). The UV-visible and PL spectra resulting from the blue emissive (B-CQD) and green emissive (G-CQD) materials are shown in Figures 1A,B with an absorption peak at ~280 nm and PL emissions at 450 and 510 nm, respectively.…”

Section: Photophysics Of Carbon Quantum Dotsmentioning

confidence: 99%

“…(C) and (D) represent the excitation-dependence spectroscopy of the blue and green CQDs at different excitation wavelengths. Panels reproduced from Singh et al ( 2018 ). Copyright 2018, Royal Society of Chemistry.…”

Section: Photophysics Of Carbon Quantum Dotsmentioning

confidence: 99%

A Perspective on Application of Carbon Quantum Dots in Luminescence Immunoassays

Hesari

Ding

2020

Front. Chem.

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“…Several protocols have been reported for AgClNPs synthesis using microorganisms such as bacteria, fungi and algae [6] [19]. To this extent, very few reports were available under the criteria of the synthesis of AgClNPs from marine resources and their application in the medicinal field [20].…”

Section: Introductionmentioning

confidence: 99%

Biogenic Synthesis of Silver Chloride Nanoparticles from Padina gymnospora and their Evaluation of Antimicrobial, Larvicidal, Hemolytic and Anticancer Activities.

Rajagopal¹,

Manivannan

Sundararajan

et al. 2020

Preprint

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In the current study, silver chloride nanoparticles (AgClNPs) have been synthesized using the aqueous extract of Padina gymnospora and further characterized by Uv-visible spectroscopy, Fourier-transform infrared spectroscopy, X-ray powder diffraction, Scanning electron microscopy, energy dispersive spectroscopy, Transmission electron microscopy and Atomic force microscopy. The synthesized silver chloride nanoparticles were found to be mono-dispersed and spherical with an average size between 11.5 to 32.86 nm. The particles also showed a cytotoxic effect in a dose-dependent manner against MCF 7 cells (IC50 = 31.37 µg/mL). Besides, it showed the larvicidal activity against Aedes aegypti at a lower dose (3.92 µg/ml) than that of the aqueous extract (13.01 µg/ml). It also exhibited significant antimicrobial activity against selected bacterial and fungal pathogens. The synthesized silver chloride nanoparticles showed the best minimum inhibitory concentration (MIC) of 8 mg/L in Canadian Albicans, followed by vancomycin resistance Enterococcus feacasli (VREF) 32 mg/L. Furthermore, the toxicity assessment by haemolytic assay revealed that AgClNPs could be safe and also used for further investigation. Therefore, the synthesized silver chloride nanoparticles may be used as a therapeutic agent for breast cancer, antimicrobial agent and also to control the dengue vector as attributed to the presence of bioactive components.

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Biocompatible fluorescent carbon quantum dots prepared from beetroot extract for in vivo live imaging in C. elegans and BALB/c mice

Abstract: Luminescent carbon quantum dots (CQDs) prepared from aqueous beetroot extract were developed as unique fluorescent nanomaterials for in vivo live animal imaging applications.

Cited by 107 publications

References 33 publications

Carbon dot-protoporphyrin IX conjugates for improved drug delivery and bioimaging

Carbon dot-protoporphyrin IX conjugates for improved drug delivery and bioimaging

A Perspective on Application of Carbon Quantum Dots in Luminescence Immunoassays

Biogenic Synthesis of Silver Chloride Nanoparticles from Padina gymnospora and their Evaluation of Antimicrobial, Larvicidal, Hemolytic and Anticancer Activities.

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