Biocompatible fluorescent nanocrystals for immunolabeling of membrane proteins and cells

Sukhanova, Alyona; Devy, Jérôme; Ventéo, Lydie; Kaplan, Hervé; Artemyev, Mikhail; Oleinikov, Vladimir; Klinov, Dmitry V.; Pluot, M; Cohen, Jacques H. M.; Nabiev, Igor

doi:10.1016/j.ab.2003.09.031

Cited by 304 publications

(204 citation statements)

References 15 publications

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“…Furthermore, the particle modification by adsorption of polyelectrolytes has been reported, either as additional layer (Sukhanova et al 2004) or replacing the original surfactant Lin et al 2008b) of different core materials. As shown by Nann, quantum dots coated with branched PEI could be dispersed in both aqueous and organic solution, the primary amino groups either binding to the particle surface or pointing outwards to solution (Nann 2005).…”

Section: (D) Polymer Coatingsmentioning

confidence: 99%

“…to hydroxyl groups (Kairdolf et al 2008a) or tertiary amines (Yezhelyev et al 2008). Primary amines are commonly introduced by partial conversation of functional groups, for instance carboxylic acids, by (partial) ligand exchange or adsorption of polyelectrolytes (Wuister et al 2003;Hoshino et al 2004;Schellenberger et al 2004;Sukhanova et al 2004;Sun et al 2006b;Susumu et al 2007;Zhang et al 2007;Liu et al 2008). At pH values below the pK a value of the amines, the particles are positively charged and can be prone to aggregation during the modification steps, e.g.…”

Section: Particle Functionalization (A) Chemical Functional Groupsmentioning

confidence: 99%

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Surface modification, functionalization and bioconjugation of colloidal inorganic nanoparticles

Sperling

Parak

2010

Phil. Trans. R. Soc. A.

1,409

1,092

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Inorganic colloidal nanoparticles are very small, nanoscale objects with inorganic cores that are dispersed in a solvent. Depending on the material they consist of, nanoparticles can possess a number of different properties such as high electron density and strong optical absorption (e.g. metal particles, in particular Au), photoluminescence in the form of fluorescence (semiconductor quantum dots, e.g. CdSe or CdTe) or phosphorescence (doped oxide materials, e.g. Y 2 O 3 ), or magnetic moment (e.g. iron oxide or cobalt nanoparticles). Prerequisite for every possible application is the proper surface functionalization of such nanoparticles, which determines their interaction with the environment. These interactions ultimately affect the colloidal stability of the particles, and may yield to a controlled assembly or to the delivery of nanoparticles to a target, e.g. by appropriate functional molecules on the particle surface. This work aims to review different strategies of surface modification and functionalization of inorganic colloidal nanoparticles with a special focus on the material systems gold and semiconductor nanoparticles, such as CdSe/ZnS. However, the discussed strategies are often of general nature and apply in the same way to nanoparticles of other materials.

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Section: (D) Polymer Coatingsmentioning

confidence: 99%

Section: Particle Functionalization (A) Chemical Functional Groupsmentioning

confidence: 99%

Surface modification, functionalization and bioconjugation of colloidal inorganic nanoparticles

Sperling

Parak

2010

Phil. Trans. R. Soc. A.

1,409

1,092

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“…This "shell" layer can increase the QY to values up to ∼90 % (Reiss et al 2002) by eliminating surface traps and better confining the exciton into the core. This step also enhances QDs' photostability by several orders of magnitude relative to conventional dyes (Sukhanova et al 2004).…”

Section: Bandgap and Wavefunction Engineeringmentioning

confidence: 99%

“…These various QD solubilization strategies include: (i) ligand exchange with simple thiol-containing molecules (Chan and Nie 1998;Pathak et al 2001) or more sophisticated ones such as oligomeric phosphines (Kim and Bawendi 2003), dendrons (Guo et al 2003), and peptides (Akerman et al 2002;Pinaud et al 2004); (ii) encapsulation by a layer of amphiphilic diblock (Wu et al 2003), or triblock copolymers (Gao et al 2004), in silica shells (Bruchez et al 1998;Gerion et al 2001), phospholipid micelles (Dubertret et al 2002), polymer beads (Gao et al 2002), polymer shells (Pellegrino et al 2004), amphiphilic polysaccharides (Osaki et al 2004); and (iii) combination of U n c o r r e c t e d P r o o f layers of different molecules conferring the required colloidal stability to QDs (Mattoussi et al 2000;Sukhanova et al 2004). Recently, some groups have developed promising water-based synthesis (Gaponik et al 2002;Rogach et al 2001) yielding particles emitting from the visible to the NIR spectrum that are intrinsically watersoluble, but have yet to be tested in biological environments.…”

Section: Quantum Dot Solubilizationmentioning

confidence: 99%

Quantum Optics: Colloidal Fluorescent Semiconductor Nanocrystals (Quantum Dots) in Single-Molecule Detection and Imaging

Bentolila

Michalet

Weiss

2008

Single Molecules and Nanotechnology

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Coming from the electronic material sciences, semiconductor nanocrystals, called quantum dots (QDs), have emerged as new powerful fluorescent probes for in vitro and in vivo biological labeling and single-molecule experiments. QDs possess several unique optical properties that make them very attractive over conventional fluorescent dyes and genetically encoded proteins technologies. They have precise emission color tunability by size due to quantum confinement effects, better photostability and brightness, wide absorption band and very narrow emission band for multiplexing, and increased fluorescence lifetimes. These characteristics, combined with some dramatic progresses achieved in surface chemistry, biocompatibility and targeting strategies have allowed their recent advances in the field of single-molecule detection and imaging

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“…These unique optical properties make QDs an excellent fluorescent probes for applications in the field of diagnosis (Bruchez et al, 1998;Chan and Nie, 1998), in vivo and in vitro imaging (Kim et al, 2003;Levene et al, 2004;Rosenthal et al, 2002), multicolor cell imaging (Hanaki et al, 2003;Jaiswal et al, 2002;Sukhanova et al, 2004;Wu et al, 2002), cell and protein tracking (Dahan et al, 2003;Voura et al, 2004), and DNA and protein sensing (Medintz et al, 2003;Zhang et al, 2005).…”

Section: Quantum Dotsmentioning

confidence: 99%

Post-Genomics Nanotechnology Is Gaining Momentum: Nanoproteomics and Applications in Life Sciences

Kobeissy

Gülbakan

Alawieh

et al. 2014

OMICS: A Journal of Integrative Biology

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The post-genomics era has brought about new Omics biotechnologies, such as proteomics and metabolomics, as well as their novel applications to personal genomics and the quantified self. These advances are now also catalyzing other and newer post-genomics innovations, leading to convergences between Omics and nanotechnology. In this work, we systematically contextualize and exemplify an emerging strand of post-genomics life sciences, namely, nanoproteomics and its applications in health and integrative biological systems. Nanotechnology has been utilized as a complementary component to revolutionize proteomics through different kinds of nanotechnology applications, including nanoporous structures, functionalized nanoparticles, quantum dots, and polymeric nanostructures. Those applications, though still in their infancy, have led to several highly sensitive diagnostics and new methods of drug delivery and targeted therapy for clinical use. The present article differs from previous analyses of nanoproteomics in that it offers an in-depth and comparative evaluation of the attendant biotechnology portfolio and their applications as seen through the lens of post-genomics life sciences and biomedicine. These include: (1) immunosensors for inflammatory, pathogenic, and autoimmune markers for infectious and autoimmune diseases, (2) amplified immunoassays for detection of cancer biomarkers, and (3) methods for targeted therapy and automatically adjusted drug delivery such as in experimental stroke and brain injury studies. As nanoproteomics becomes available both to the clinician at the bedside and the citizens who are increasingly interested in access to novel post-genomics diagnostics through initiatives such as the quantified self, we anticipate further breakthroughs in personalized and targeted medicine.

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Biocompatible fluorescent nanocrystals for immunolabeling of membrane proteins and cells

Cited by 304 publications

References 15 publications

Surface modification, functionalization and bioconjugation of colloidal inorganic nanoparticles

Surface modification, functionalization and bioconjugation of colloidal inorganic nanoparticles

Quantum Optics: Colloidal Fluorescent Semiconductor Nanocrystals (Quantum Dots) in Single-Molecule Detection and Imaging

Post-Genomics Nanotechnology Is Gaining Momentum: Nanoproteomics and Applications in Life Sciences

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