Biocompatible fluorescent silk fibroin bioink for digital light processing 3D printing

Lee, Young Jin; Lee, Joong Seob; Ajiteru, Olatunji; Lee, Ok Joo; Lee, Ji Seung; Lee, Hanna; Kim, Seong Wan; Park, Jong Woo; Kim, Kee Young; Choi, Kyu Young; Hong, Heesun; Sultan, Tipu; Kim, Soon Hee; Park, Chan Hum

doi:10.1016/j.ijbiomac.2022.05.123

Cited by 29 publications

(9 citation statements)

References 58 publications

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“…S1†) revealed that the GMA-SF possessed the proton peaks for the methacrylate vinyl group ( δ = 6.2–6.0 and 5.8–5.6 ppm) and the methyl group ( δ = 1.8 ppm), which demonstrated the successful functionalization of the methacrylate groups on RSF. 31 Subsequently, the GMA-SF solution was photo-crosslinked to obtain the RSF hydrogel. In particular, the β-sheet structure is a crucial factor that may influence the biocompatibility and degradation rate of the RSF-based materials.…”

Section: Resultsmentioning

confidence: 99%

Facilely printed silk fibroin hydrogel microparticles as injectable long-lasting fillers

Xie,

Yang,

Zheng

et al. 2024

Biomater. Sci.

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Section: Resultsmentioning

confidence: 99%

Facilely printed silk fibroin hydrogel microparticles as injectable long-lasting fillers

Xie,

Yang,

Zheng

et al. 2024

Biomater. Sci.

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“…Images of the human ear are captured under bright and fluorescent fields using a nighttime filter camera. 60 ACMMA indicates auricular chondrocytes and a bioactive bioink based on biomimetic microporous methacrylate-modified acellular cartilage matrix; APH, Alginate-PCL-HA; HA, hydroxyapatite; PCL, polycaprolactone.…”

Section: Extrusion-based 3d Bioprintingmentioning

confidence: 99%

Current Status of Auricular Reconstruction Strategy Development

Wei,

Li,

Xie

et al. 2023

Journal of Craniofacial Surgery

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Microtia has severe physical and psychological impacts on patients, and auricular reconstruction offers improved esthetics and function, alleviating psychological issues. Microtia is a congenital disease caused by a multifactorial interaction of environmental and genetic factors, with complex clinical manifestations. Classification assessment aids in determining treatment strategies. Auricular reconstruction is the primary treatment for severe microtia, focusing on the selection of auricular scaffold materials, the construction of auricular morphology, and skin and soft tissue scaffold coverage. Autologous rib cartilage and synthetic materials are both used as scaffold materials for auricular reconstruction, each with advantages and disadvantages. Methods for achieving skin and soft tissue scaffold coverage have been developed to include nonexpansion and expansion techniques. In recent years, the application of digital auxiliary technology such as finite element analysis has helped optimize surgical outcomes and reduce complications. Tissue-engineered cartilage scaffolds and 3-dimensional bioprinting technology have rapidly advanced in the field of ear reconstruction. This article discusses the prevalence and classification of microtia, the selection of auricular scaffolds, the evolution of surgical methods, and the current applications of digital auxiliary technology in ear reconstruction, with the aim of providing clinical physicians with a reference for individualized ear reconstruction surgery. The focus of this work is on the current applications and challenges of tissue engineering and 3-dimensional bioprinting technology in the field of ear reconstruction, as well as future prospects.

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“…Despite using FSF in several biomedical applications, the fabrication of a 3D form of fluorescent hydrogel has not yet been achieved due to the reduction in its fluorescent properties in gel structures [ 113 ]. Lee et al recently reported a fluorescent SF-based 3D DLP printable bio-ink using FSF from genetically modified silkworms (B. mori) [ 114 ]. The glycidyl methacrylated fluorescent SF (FSGMA) was obtained through the covalent conjugation of FSF with GMA.…”

Section: Applications Of 3d Dlp-printable Sf-based Bio-inkmentioning

confidence: 99%

“…White arrows indicate the presence of cell movement. Adapted from [ 114 ] with permission from Elsevier.…”

Section: Figurementioning

confidence: 99%

Three-Dimensional Digital Light-Processing Bioprinting Using Silk Fibroin-Based Bio-Ink: Recent Advancements in Biomedical Applications

Sultan

Lee

et al. 2022

Biomedicines

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Three-dimensional (3D) bioprinting has been developed as a viable method for fabricating functional tissues and organs by precisely spatially arranging biomaterials, cells, and biochemical components in a layer-by-layer fashion. Among the various bioprinting strategies, digital light-processing (DLP) printing has gained enormous attention due to its applications in tissue engineering and biomedical fields. It allows for high spatial resolution and the rapid printing of complex structures. Although bio-ink is a critical aspect of 3D bioprinting, only a few bio-inks have been used for DLP bioprinting in contrast to the number of bio-inks employed for other bioprinters. Recently, silk fibroin (SF), as a natural bio-ink material used for DLP 3D bioprinting, has gained extensive attention with respect to biomedical applications due to its biocompatibility and mechanical properties. This review introduces DLP-based 3D bioprinting, its related technology, and the fabrication process of silk fibroin-based bio-ink. Then, we summarize the applications of DLP 3D bioprinting based on SF-based bio-ink in the tissue engineering and biomedical fields. We also discuss the current limitations and future perspectives of DLP 3D bioprinting using SF-based bio-ink.

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Biocompatible fluorescent silk fibroin bioink for digital light processing 3D printing

Cited by 29 publications

References 58 publications

Facilely printed silk fibroin hydrogel microparticles as injectable long-lasting fillers

Facilely printed silk fibroin hydrogel microparticles as injectable long-lasting fillers

Current Status of Auricular Reconstruction Strategy Development

Three-Dimensional Digital Light-Processing Bioprinting Using Silk Fibroin-Based Bio-Ink: Recent Advancements in Biomedical Applications

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