Biocompatible Hydrogels for Microarray Cell Printing  and Encapsulation

Datar, Akshata; Joshi, Pranav; Lee, Moo‐Yeal

doi:10.3390/bios5040647

Cited by 30 publications

(20 citation statements)

References 87 publications

(137 reference statements)

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“…For this purpose, dECM was extracted using the same procedure, but biofabrication was performed through layer-by-layer rather than hollow-type squares. The upper layer was deposited in the lower layer at a 90 angle to form a lattice, resulting in a size 10 Â 10 Â 3 mm [18,19]. As shown in Figure 3, maintaining the 3D construct at 37 C resulted in gelation so that the shape of the construct was maintained even after 7 days, and encapsulated HDFs were also shown to have maintained their morphology and proliferation.…”

Section: Discussionmentioning

confidence: 99%

A potential dermal substitute using decellularized dermis extracellular matrix derived bio-ink

Won¹,

Lee²,

Kim³

et al. 2019

Artificial Cells, Nanomedicine, and Biotechnology

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Upon bioprinting, cells are mixed with a biomaterial to fabricate a living tissue, thus emphasizing the importance of biomaterials. The biomaterial used in this study was a bio-ink prepared using skin decellularized extracellular matrix (dECM). Skin dECM was extracted by treating the dermis with chemicals and enzymes; the basic structural and functional proteins of the ECM, including collagen, glycosaminoglycans (GAGs), bioreactive materials and growth factors, were preserved, whereas the resident cells that might cause immune rejection or inflammatory responses were removed. The bio-ink based on dECM powder, together with human dermal fibroblasts (HDFs), was loaded into the nozzle of the 3D bioprinter to create the 3D construct. This construct underwent gelation with changing temperature while its shape was maintained for 7 days. The cells showed over 90% viability and proliferation. By analysing the gene expression pattern in the cells of the construct, the skin regenerative mechanism of the bio-ink was verified. Microarray results confirmed that the gene expression related to skin morphology and development had been enhanced because the bioreactive molecules and growth factors, in addition to residual ECM in dECM, provided an optimal condition for the HDFs.

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Section: Discussionmentioning

confidence: 99%

A potential dermal substitute using decellularized dermis extracellular matrix derived bio-ink

Won¹,

Lee²,

Kim³

et al. 2019

Artificial Cells, Nanomedicine, and Biotechnology

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show abstract

“…[ 31 ] Additionally, their anti‐biofouling character provides an infection‐free environment for cells. [ 18–21,32–34 ] Preventing, controlling, and resisting nonspecific protein adsorption and cell/bacterial adhesion can improve the performance of biomedical devices and prolong their service life in the body.…”

Section: Introductionmentioning

confidence: 99%

Hydrogel−Solid Hybrid Materials for Biomedical Applications Enabled by Surface‐Embedded Radicals

Walia

Akhavan

Kosobrodova

et al. 2020

Adv Funct Materials

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Combinations of hydrogels and solids provide high level functionality for devices such as tissue engineering scaffolds and soft machines. However, the weak bonding between hydrogels and solids hampers functionality. Here, a versatile strategy to develop mechanically robust solid−hydrogel hybrid materials using surface embedded radicals generated through plasma immersion ion implantation (PIII) of polymeric surfaces is reported. Evidence is provided that the reactive radicals play a dual role: inducing surface‐initiated, spontaneous polymerization of hydrogels; and binding the hydrogels to the surfaces. Acrylamide and silk hydrogels are formed and covalently attached through spontaneous reactions with the radicals on PIII activated polymer surfaces without cross‐linking agents or initiators. The hydrogel amount increases with incubation time, monomer concentration, and temperature. Stability tests indicate that 95% of the hydrogel is retained even after 4 months in PBS solution. T‐peel tests show that failure occurs at the tape−hydrogel interface and the hydrogel‐PIII‐treated PTFE interfacial adhesion strength is over 300 N m−1. Cell assays show no adhesion to the as‐synthesized hydrogels; however, hydrogels synthesized with fibronectin enable cell adhesion and spreading. These results show that polymers functionalized with surface‐embedded radicals provide excellent solid platforms for the generation of robust solid−hydrogel hybrid structures for biomedical applications.

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“…Solenoid valve assisted printing uses a valve that has the ability to open in the presence of an electromagnetic force to eject fluid droplets (Datar, Joshi, & Lee, 2015) (Figure 2). Modifying the time that the valve is open enables control over the size and volume of the dispensed droplet.…”

Section: Bioprinting Devices For 3d Cell Culturementioning

confidence: 99%

Three‐dimensional in vitro cell culture devices using patient‐derived cells for high‐throughput screening of drug combinations

et al. 2020

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Drug discovery using traditional animal models is often inefficient because test animal models cannot accurately represent human responses, particularly in terms of toxicology and pathophysiology. For these reasons, there is an urgent need to develop in vitro human disease models to accelerate new drug development. This is especially the case for precision medicine, where current in vitro and ex vivo models are not sufficient to predict individual drug efficacy and safety across the enormous heterogeneity of populations and individuals. In addition, model cell lines cultured under non‐physiological conditions do not recapitulate the complex microenvironment of patient tissues. Precision medicine using patient‐derived cells and drug combination therapies may be a promising solution to overcome these limitations. Three‐dimensional (3D) cell culture models can simultaneously simulate complex in vivo tissue microenvironments and be miniaturized for large‐scale screening of drug combinations using limited available patient‐derived cells. Recently, microfluidic and microarray platforms have been developed to mimic multiple human organs and human‐specific disease states, and are becoming more commonly used in high‐throughput drug screening. Herein, we highlight the current status of microfluidic‐based 3D cell culture devices, such as organ/body‐on‐chip systems, as well as microarray‐based 3D cell culture platforms, which can be combined with patient‐derived cells. Miniaturized cell culture systems using patient‐derived cells represent promising tools for identifying new drug combinations and also biological mechanisms that underlie drug synergies.

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Biocompatible Hydrogels for Microarray Cell Printing and Encapsulation

Cited by 30 publications

References 87 publications

A potential dermal substitute using decellularized dermis extracellular matrix derived bio-ink

A potential dermal substitute using decellularized dermis extracellular matrix derived bio-ink

Hydrogel−Solid Hybrid Materials for Biomedical Applications Enabled by Surface‐Embedded Radicals

Three‐dimensional in vitro cell culture devices using patient‐derived cells for high‐throughput screening of drug combinations

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