Biocompatible Materials Based on Self-Assembling Peptides on Ti25Nb10Zr Alloy: Molecular Structure and Organization Investigated by Synchrotron Radiation Induced Techniques

Abstract

In this work, we applied advanced Synchrotron Radiation (SR) induced techniques to the study of the chemisorption of the Self Assembling Peptide EAbuK16, i.e., H-Abu-Glu-Abu-Glu-Abu-Lys-Abu-Lys-Abu-Glu-Abu-Glu-Abu-Lys-Abu-Lys-NH2 that is able to spontaneously aggregate in anti-parallel β-sheet conformation, onto annealed Ti25Nb10Zr alloy surfaces. This synthetic amphiphilic oligopeptide is a good candidate to mimic extracellular matrix for bone prosthesis, since its β-sheets stack onto each other in a multilayer oriented nanostructure with internal pores of 5–200 nm size. To prepare the biomimetic material, Ti25Nb10Zr discs were treated with aqueous solutions of EAbuK16 at different pH values. Here we present the results achieved by performing SR-induced X-ray Photoelectron Spectroscopy (SR-XPS), angle-dependent Near Edge X-ray Absorption Fine Structure (NEXAFS) spectroscopy, FESEM and AFM imaging on Ti25Nb10Zr discs after incubation with self-assembling peptide solution at five different pH values, selected deliberately to investigate the best conditions for peptide immobilization.

Antibacterial Mesoporous Titania Films with Embedded Gentamicin and Surface Modified with Bone Morphogenetic Protein 2 to Promote Osseointegration in Bone Implants

“…Depending on the type of bone, the modulus of elasticity varies between 4 to 30 GPa . Titanium and titanium alloys are recognized as good materials for orthopedic implants due to their osseocompatibility and bone‐bonding capacity . Currently used commercial titanium implants satisfy the required mechanical properties with a Young's modulus within the range of 100–120 GPa .…”

Macromolecule Orientation in Nanofibers

“…Nature always uses the simplest material blocks, which has rather meager properties, to design multi-scale materials with outstanding bio-functional properties, providing scientists with infinite inspiration to fabricate bio-inspired hierarchical structural materials using the simplest possible material blocks to achieve the highest performance. Molecular movement in some molecular devices can be effectively controlled by chemical methods like molecular rotary motors [ 11 ], synchrotron radiation-induced techniques [ 12 ], and molecular motors [ 13 ]. A mechanical technique using the simplest possible laminar flow has never been used before.…”

Hydroxyapatite Surfaces Functionalized with a Self-Assembling Peptide: XPS, RAIRS and NEXAFS Study

“…HAP samples were incubated in SAP solutions with pH values of 4 and 10 pH, as described in the experimental section. Previous investigations on SAP adsorption on the TiO 2 surface showed that the thickness of the peptide overlayer decreases with increasing pH of the mother solution [23,37]; however, in the 4-10 pH range, the thickness of the peptide overlayer remains approximately constant, and only at pH = 12 a significant decrease of the overlayer thickness can be observed [23]. On the other hand, the solubility of hydroxyapatite is expected to increase as the solution's pH decreases.…”