Biocompatible Ni‐doped CdSe/ZnS semiconductor nanocrystals for cellular imaging and sorting

Abstract

Quantum dots (QD) with chemical composition QD 650CdSe=ZnS_Ni were successfully synthesized using a hydrothermal method and chemical precipitation. The nanocrystalline phase of the nanostructures was isolated and characterized using X-ray diffraction (XRD). The mean crystalline size doped core/shell Ni-dopant range was 9.0 ± 2.0 nm. The ferromagnetic data revealed the magnetic behaviour of QD 650CdSe=ZnS_Ni . The optical absorption measurements of these QDs were in the UV-visible light range 200-800 nm for a band gap value of 2.11 eV for QD 650 CdSe=ZnS_Ni . This means that pure QD 650CdSe and QD 650 CdSe=ZnS_Ni underwent a redshift when compared with bulk CdSe. For QD 650CdSe=ZnS_Ni there was successful uptake by cell lines including HeLa and MCF-7 for bioimaging and sorting applications.

Anti-cancer activity of crude Slrp protein conjugated mesoporous silica nanoparticles in HeLa Cell Lines: An in vitro approach

“…The MSN N were further modified by incorporating a monodentate thiol ligand, specifically β-MPA, onto the surface to introduce a negative charge with the modified previously published protocol (Wang et al,2015;Vyshnava et al,2022a;Vyshnava et al, 2020;Vyshnava et al,2022c). MSN N were thoroughly cleaned by dispersing them in ethanol and subjected the mixture to ultrasonication bath for one hour.…”

Deciphering breast cancer cell heterogeneity: a quantum dot-conjugate approach employing MCF-7 and THP-1 co-cultures

“…The detailed preparation of the quantum dot antibody conjugates (QD l/Ab ) is explained in the ESI † (Section S.1). All of the QD l/Ab are prepared in the sequential process, including the synthesis of quantum dots (QD l ) with fluorescence emissions at 450 nm (blue-QD 450 ), 525 nm (green-QD 525 ), and 615 nm (red-QD 615 ), as shown in the earlier research of Vyshnava et al 27,30,31,67 Later, the surfaces of the QD l were modified with MPA using the biphasic ligand exchange to form active -COO À (QD l/MPA ). The EDC/NHS chemical coupling chemical reaction was acquired for (PEG) on the surface of the QD l/MPA (QD l/MPA/PEG ) to form stable and low cytotoxicity.…”