Biocompatible pectin-based hybrid hydrogels for tissue engineering applications

Tortorella, Silvia; Inzalaco, Giovanni; Dapporto, Francesca; Maturi, Mirko; Sambri, Letizia; Buratti, Veronica Vetri; Chiariello, Mario; Franchini, Mauro Comes; Locatelli, Erica

doi:10.1039/d1nj04142h

Cited by 17 publications

(15 citation statements)

References 38 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Beyond that, the improved biological properties and high mechanical strength of CNC-reinforced polymeric networks are mainly attributed to the crystalline structure and the formation of inter- and intramolecular hydrogel bonding in the networks . Often, synthetic polymers (e.g., Pluronic, poly(oligoethylene glycol methacrylate) and natural polymers (e.g., hyaluronic acid, chitosan, and pectin) have been formulated with CNCs to prepare bionanocomposites for various biomedical applications. − Therefore, uniting the unique features of CNCs with synthetic hydrogels could overcome the shortcomings of each other and may help to prepare a more advanced hydrogel system for biomedical applications.…”

Section: Introductionmentioning

confidence: 99%

Cellulose Nanocrystals-Incorporated Thermosensitive Hydrogel for Controlled Release, 3D Printing, and Breast Cancer Treatment Applications

Phan

Murugesan

Huong

et al. 2022

ACS Appl. Mater. Interfaces

View full text Add to dashboard Cite

In situ-gel-forming thermoresponsive copolymers have been widely exploited in controlled delivery applications because their critical gel temperature is similar to human body temperature. However, there are limitations to controlling the delivery of biologics from a hydrogel network because of the poor networking and reinforcement between the copolymer networks. This study developed an in situ-forming robust injectable and 3D printable hydrogel network based on cellulose nanocrystals (CNCs) incorporated amphiphilic copolymers, poly(ε-caprolactoneco-lactide)-b-poly(ethylene glycol)-b-poly(ε-caprolactone-co-lactide (PCLA). In addition, the physicochemical and mechanical properties of injectable hydrogels were controlled by physically incorporating CNCs with amphiphilic PCLA copolymers. CNCs played an unprecedented role in physically reinforcing the PCLA copolymers' micelle network via intermicellar bridges. Apart from that, the free-flowing closely packed rod-like CNCs incorporated PCLA micelle networks at low temperature transformed to a stable viscoelastic hydrogel network at physiological temperature. CNC incorporated PCLA copolymer sols effectively coordinated with hydrophobic doxorubicin and water-soluble lysozyme by a combination of hydrophobic and hydrogen bonding interaction and controlled the release of biologics. As shown by the 3D printing results, the biocompatible PCLA hydrogels continuously extruded during printing had good injectability and maintained high shape fidelity after printing without any secondary cross-linking steps. The interlayer bonding between the printed layers was high and formed stable 3D structures up to 10 layers. Subcutaneous injection of free-flowing CNC incorporated PCLA copolymer sols to BALB/c mice formed a hydrogel instantly and showed controlled biodegradation of the hydrogel depot without induction of toxicity at the implantation sites or surrounding tissues. At the same time, the in vivo antitumor effect on the MDA-MB-231 tumor xenograft model demonstrated that DOX-loaded hydrogel formulation significantly inhibited the tumor growth. In summary, the CNC incorporated biodegradable hydrogels developed in this study exhibit a prolonged release with special release kinetics for hydrophobic and hydrophilic biologics.

show abstract

Section: Introductionmentioning

confidence: 99%

Cellulose Nanocrystals-Incorporated Thermosensitive Hydrogel for Controlled Release, 3D Printing, and Breast Cancer Treatment Applications

Phan

Murugesan

Huong

et al. 2022

ACS Appl. Mater. Interfaces

View full text Add to dashboard Cite

show abstract

“…Pectin is widely used as a wound dressing [ 22 ]. Alginate [ 23 ] and pectin-based hybrid hydrogels are appealing for wound dressing applications [ 24 ].…”

Section: Introductionmentioning

confidence: 99%

Development of Nanoemulsions for Wound Dressings Containing Cassia alata L. Leaf Extraction

Sangkaew

Wanmasae

Bunluepeuch

et al. 2022

Evidence-Based Complementary and Alternative Medicine

View full text Add to dashboard Cite

Natural polymer-based hydrogel films possess considerable potential for use in biomedical applications and are excellent for wound healing. The purpose of this research was to use ionic crosslinking to improve the mechanical characteristics, absorption of fluid in the wound, and drug release behavior of Cassia alata L. (CA) extract loaded niosomes (CANs) that were incorporated in an alginate-pectin film (A/P). Then, chemically crosslinked A/P hydrogels were obtained by immersing them in different concentrations of calcium chloride (CaCl2) (0.5–1% w/v) for 15–120 s. The degree of crosslinking was controlled by both contact time and CaCl2 concentration. The optimal crosslinking conditions were 1% CaCl2 for 15 seconds. In this study, the following features of the hydrogel films were investigated: physical properties, morphological characteristics, drug loading, in vitro drug release, antibacterial activity, wound healing activity, cytocompatibility profiles, and hemocompatibility. The crosslinked hydrogel films maintained their physical integrity during use, with the 1% film attaining the best results in the shortest period (15 sec). Then, in vitro drug release from the films was examined. Crosslinking was observed to prolong the release of the CA extract from the hydrogel film. Finally, a cell viability experiment was conducted to evaluate the cytotoxicity profile. The A/P composite film exhibited excellent wound dressing qualities and good mechanical properties in preformulation testing. The in vitro drug release profile indicated that the A/P created a regulated drug release profile, and the cell viability experiment revealed that the film was nontoxic and hemocompatible. A/P composite films can be produced using CAN extract as a possible wound dressing. However, further studies in animals and humans are required to determine both safety and effectiveness.

show abstract

“…Since the mechanical properties of hydrogels can be widely adjusted by changing the polymer chemistry and cross-linker density [4,5], they have found wide-spread applications. The ability of external stimuli to trigger swelling that drives large deformations has been harnessed to drive microfluidic pumps [6,7] and has led to their use as micro-actuators for optical, flow control, sensor and microrobotics applications [8,9].…”

mentioning

confidence: 99%

“…In tissue engineering, they are used as a matrix framework for the repair and regeneration of a wide range of tissues and organs [16][17][18]. Hydrogel wound dressings have been developed that maintain a moist healing environment whilst allowing gaseous exchange, promoting wound healing [4,19,20]. These systems, characterised by complex interactions between biological materials and hydrogels, require a thorough understanding of hydrogel swelling behaviour.…”

mentioning

confidence: 99%

“…Inspired by the flat geometries found in cell layers [21], wound dressing [4] and hydrogel water harvesters [33], in this letter we investigate experimentally and theoretically the absorption and spreading of a single droplet of water into a thin hydrogel layer. This is also a model system for the absorption of more hazardous substances into thin layers of paints and coatings [34].…”

mentioning

confidence: 99%

See 1 more Smart Citation

Droplet absorption and spreading into thin layers of polymer hydrogels

Etzold¹,

Fortune²,

Landel³

et al. 2022

Preprint

View full text Add to dashboard Cite

From biological tissues to layers of paint, materials with the capacity to swell are ubiquitous.Here, we study experimentally and theoretically one of the conceptually simplest of such systems, the swelling of a thin hydrogel layer by a single water drop. Using a bespoke experimental setup, we observe fast absorption leading to a radially spreading axisymmetric blister. Employing a linear poroelastic framework and thin-layer scalings, we develop a non-linear one-dimensional diffusion equation for the evolution of the blister height profile, which agrees well with experimental observations. .

show abstract

Biocompatible pectin-based hybrid hydrogels for tissue engineering applications

Abstract: Hybrid hydrogels made of chemically modified pectin, gelatin and xanthan gum have been formulated and processed through a double crosslinking step, aimed at wound healing applications.

Cited by 17 publications

References 38 publications

Cellulose Nanocrystals-Incorporated Thermosensitive Hydrogel for Controlled Release, 3D Printing, and Breast Cancer Treatment Applications

Cellulose Nanocrystals-Incorporated Thermosensitive Hydrogel for Controlled Release, 3D Printing, and Breast Cancer Treatment Applications

Development of Nanoemulsions for Wound Dressings Containing Cassia alata L. Leaf Extraction

Droplet absorption and spreading into thin layers of polymer hydrogels

Contact Info

Product

Resources

About