Biocompatible polysaccharide-based cryogels

Reichelt, Senta; Becher, Jana; Weisser, Jürgen; Prager, Andrea; Decker, Ulrich; Möller, Stephanie; Berg, Albrecht; Schnabelrauch, Matthias

doi:10.1016/j.msec.2013.10.034

Cited by 45 publications

(28 citation statements)

References 56 publications

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“…The presence of the rounded cells is typical of cells cultured on soft planar HA based hydrogels [51], and macro-porous HA hydrogels. [42] Cells grown on modified HA cryogels ( Figure 6A), however, exhibited significantly smaller cell clumps in comparison to unmodified HA cryogels ( Figure 6B), a trend which was observed over several days in culture, and seen in repeat experiments across both fibronectin (supp.…”

Section: Bio-modification and Cell Loadingmentioning

confidence: 78%

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Formation and characterisation of a modifiable soft macro-porous hyaluronic acid cryogel platform

Henderson

Ladewig

Haylock

et al. 2015

Journal of Biomaterials Science, Polymer Edition

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A facile method for the synthesis of cell supportive, highly macro-porous hyaluronic acid (HA) hydrogels via cryogelation is presented. Unmodified HA was chemically cross-linked via EDC/NHS zero-length cross-linking at sub-zero temperatures to yield cryogels with high porosity and high pore interconnectivity. The physical properties of the HA cryogels including porosity, average pore size, elasticity and swelling properties were characterised as a function of cryogelation conditions and composition of the precursor solution. The HA cryogels swell extensively in water, with the average porosities observed being ~90% under all conditions explored. The morphology of the cryogels can be controlled, allowing scaffolds with an average pore size ranging from 18 ± 2 to 87 ± 5 μm to be formed. By varying the cross-linking degree and HA concentration, a wide range of bulk elastic properties can be achieved, ranging from ~1 kPa to above 10 kPa. Preliminary cell culture experiments, with NIH 3T3 and HEK 293 cell lines, performed on biochemically modified and unmodified gels show the cryogels support cell proliferation and cell interactions, illustrating the biomedical potential of the platform.

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Section: Bio-modification and Cell Loadingmentioning

confidence: 78%

“…Typical of cryogels, HA cryogels reach their equilibrium-swelling ratio in less than 5 minutes. [42,43] Storage of these samples overnight in PBS had no significant impact upon their equilibrium-swelling ratio. As summarized in Table 2, swelling is primarily impacted by polymer content and crosslink density.…”

Section: Swelling Propertiesmentioning

confidence: 99%

Formation and characterisation of a modifiable soft macro-porous hyaluronic acid cryogel platform

Henderson

Ladewig

Haylock

et al. 2015

Journal of Biomaterials Science, Polymer Edition

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“…The present paper describes the fabrication of original super‐macroporous dextran cryogels via cryogenic treatment and UV‐induced crosslinking in frozen semi‐dilute/concentrated aqueous solutions of high‐molar‐mass dextran. As far as we know, dextran cryogels have been obtained only from modified dextran like dextran methacrylate and oxidized dextran . This paper reports the first work on direct chemical crosslinking of non‐modified dextran using UV light.…”

Section: Introductionmentioning

confidence: 99%

Super-macroporous dextran cryogels via UV-induced crosslinking: synthesis and characterization

et al. 2017

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Cryogenic treatment and UV irradiation were exploited for the preparation of super‐macroporous cryogels from non‐modified high‐molar‐mass dextran. The photo‐crosslinking process was initiated by (4‐benzoylbenzyl)trimethylammonium chloride and N,N′‐methylenebisacrylamide (BAAm) was used as a crosslinking agent. Gel fraction yield and degree of swelling of the dextran cryogels were determined gravimetrically. Cryogel morphology and mechanical properties were studied using environmental scanning electron microscopy and dynamic rheological measurements, respectively. The effects of dextran concentration in the initial polymer solution, polymer molar mass and BAAm content on the crosslinking efficacy, physico‐mechanical properties and morphology of the cryogels were evaluated. The dextran cryogels were assessed as carriers of the model water‐soluble drug metoprolol. © 2017 Society of Chemical Industry

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“…They were then frozen and irradiated directly with electron beam irradiation to achieve crosslinking. The resultant cryogels were found to be highly biocompatible and readily allowed for ingrowth of 3T3 cells (Reichelt et al 2014). …”

Section: Epoxide-acrylatementioning

confidence: 99%