Biocompatible ratiometric fluorescent chemosensor for ultrasensitive detection of endogenous aminopeptidase N In Vitro and In Vivo

Ma, Minglong; Dai, Dianfeng; Ma, Pinyi; Wu, Qiong; Gao, Dejiang; Song, Daqian

doi:10.1016/j.snb.2022.133228

Cited by 7 publications

(6 citation statements)

References 53 publications

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“…Recently, many APN response fluorescent probes have been developed, their structures are shown in Figure 7. [45][46][47][48][49][50][51][52][53][54][55][56][57][58][59][60][61][62][63] Coumarin and its derivatives are traditional bluefluorescence labels, and many probes are designed with them. The cutting-edge design of these probes primarily focuses on creating an amide substrate through the interaction of L-leucine or L-alanine with a colorimetric or fluorescent reporter, such as β-naphthylamine, p-nitroaniline, or 7-amino-4-methylcoumarin.…”

Section: Fluorescent Probesmentioning

confidence: 99%

“…Song et al developed Probe 34 in 2023. [62] This probe consists of Nile Blue as the fluorophore and L-Alanine as the recognizing site. This long wavelength probe emits its signal at 675 nm and 610 nm, with a ratiometric signal starting at the detection limit of 15 pg/mL.…”

Section: Fluorescent Probesmentioning

confidence: 99%

“…Since APN expression is highly related to tumors, fluorescent probe design can be applied to tumor imaging. Recently, many APN response fluorescent probes have been developed, their structures are shown in Figure 7 [45‐63] …”

Section: Apn‐responsive Small‐molecular Probesmentioning

confidence: 99%

“…Song et al. developed Probe 34 in 2023 [62] . This probe consists of Nile Blue as the fluorophore and L‐Alanine as the recognizing site.…”

Section: Apn‐responsive Small‐molecular Probesmentioning

confidence: 99%

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Recent Advances of Aminopeptidases‐Responsive Small‐Molecular Probes for Bioimaging

Xu,

Cui,

et al. 2024

Chemistry An Asian Journal

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Aminopeptidases, enzymes with critical roles in human body, are emerging as vital biomarkers for metabolic processes and diseases. Aberrant aminopeptidase levels are often associated with diseases, particularly cancer. Small‐molecule probes, such as fluorescent, fluorescent/photoacoustics, bioluminescent, and chemiluminescent probes, are essential tools in the study of aminopeptidases‐related diseases. The fluorescent probes provide real‐time insights into protein activities, offering high sensitivity in specific locations, and precise spatiotemporal results. Additionally, photoacoustic probes offer signals that are able to penetrate deeper tissues. Bioluminescent and chemiluminescent probes can enhance in vivo imaging abilities by reducing the background. This comprehensive review is focused on small‐molecule probes that respond to four key aminopeptidases: aminopeptidase N, leucine aminopeptidase, Pyroglutamate aminopeptidase 1, and Prolyl Aminopeptidase, and their utilization in imaging tumors and afflicted regions. In this review, the design strategy of small‐molecule probes, the variety of designs from previous studies, and the opportunities of future bioimaging applications are discussed, serving as a roadmap for future research, sparking innovations in aminopeptidase‐responsive probe development, and enhancing our understanding of these enzymes in disease diagnostics and treatment.

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Section: Fluorescent Probesmentioning

confidence: 99%

Section: Fluorescent Probesmentioning

confidence: 99%

Section: Apn‐responsive Small‐molecular Probesmentioning

confidence: 99%

“…Song et al. developed Probe 34 in 2023 [62] . This probe consists of Nile Blue as the fluorophore and L‐Alanine as the recognizing site.…”

Section: Apn‐responsive Small‐molecular Probesmentioning

confidence: 99%

See 2 more Smart Citations

Recent Advances of Aminopeptidases‐Responsive Small‐Molecular Probes for Bioimaging

Xu,

Cui,

et al. 2024

Chemistry An Asian Journal

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“…However, many of these methods are limited by expensive instruments, complex techniques, and a lengthy preparation time. In contrast, optical analysis is a cost-effective, user-friendly method with direct signal readout and the potential for combination with other detection methods. , Li’s group utilized high quantum yield N-doped carbon dots as fluorophores for phosphatase detection via the internal filtration effect, which was also successfully applied for ALP inhibitor studies . Guo’s group designed blue fluorescent N, S codoped carbon dots (N/S-CDs) and employed ALP-induced formation of yellow fluorescent product 2,3-diaminobenzidine (DPA) to visually characterize ALP activity and its inhibitors .…”

Section: Introductionmentioning

confidence: 99%

Alkaline Phosphatase-Decorated Au Nanoparticles Immobilized on Bi₂S₃ Particles Combined with Ag₂S Quantum Dots as a Photoelectrochemical Sensing Platform for Alkaline Phosphatase Activity and Fluoride Ions

Zhao,

Chen,

Zhou

et al. 2024

ACS Appl. Nano Mater.

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A photoelectrochemical (PEC) sensing platform for the detection of alkaline phosphatase (ALP) and its inhibitor F − ions was developed based on the Ag 2 S/Bi 2 S 3 composite. The signal variation strategy was triggered by the in situ formation and consumption of the electron donor. Ag 2 S quantum dots (QDs) on the Bi 2 S 3 surface effectively suppress the recombination of photogenerated carriers, leading to an enhanced photocurrent response. After ALP is immobilized on the Ag 2 S/Bi 2 S 3 surface, the in situ catalyzed hydrolysis of L-ascorbic acid 2-phosphate trisodium salt (AAP) appeared, resulting in the production of ascorbic acid (AA). AA acts as an electron donor, enhancing the photocurrent and offering a strategy for quantifying ALP activity. Sodium fluoride (NaF) induces a reduction in ALP activity, and by exposing the sensing platform to various concentrations of NaF, the inhibition of ALP can be identified. The developed PEC sensor demonstrates outstanding detection performance for NaF, featuring a linear concentration range from 100 pM to 20 μM and a low detection limit of 1.28 pM. The integration of Ag 2 S/Bi 2 S 3 to the in situ formation of electron donors in PEC technology presents an innovative approach for detecting ALP activity and its inhibitors. This platform delivers enhanced sensitivity and selectivity, making it valuable for diverse applications in detecting enzyme activity and screening their inhibitors in biofluid and food samples.

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Activatable Fluorescence and Bio/Chemiluminescence Probes for Aminopeptidases: From Design to Biomedical Applications

Wu,

Deng,

et al. 2024

Advanced Materials

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Aminopeptidases are exopeptidases that catalyze the cleavage of amino acid residues from the N‐terminal fragment of protein or peptide substrates. Owing to their function, they play important roles in protein maturation, signal transduction, cell‐cycle control, and various disease mechanisms, notably in cancer pathology. To gain better insights into their function, molecular imaging assisted by fluorescence and bio/chemiluminescence probes has become an indispensable method to their superiorities, including excellent sensitivity, selectivity, and real‐time and noninvasive imaging. Numerous efforts are made to develop activatable probes that can effectively enhance efficiency and accuracy as well as minimize the side effects. This review is classified according to the type of aminopeptidases, summarizing some recent works on the design, work mechanism, and sensing, imaging, and theranostic performance of their activatable probe. Finally, the current challenges are outlined in developing activatable probes for aminopeptidases and provide possible solutions for future advancements.

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Biocompatible ratiometric fluorescent chemosensor for ultrasensitive detection of endogenous aminopeptidase N In Vitro and In Vivo

Cited by 7 publications

References 53 publications

Recent Advances of Aminopeptidases‐Responsive Small‐Molecular Probes for Bioimaging

Recent Advances of Aminopeptidases‐Responsive Small‐Molecular Probes for Bioimaging

Alkaline Phosphatase-Decorated Au Nanoparticles Immobilized on Bi₂S₃ Particles Combined with Ag₂S Quantum Dots as a Photoelectrochemical Sensing Platform for Alkaline Phosphatase Activity and Fluoride Ions

Activatable Fluorescence and Bio/Chemiluminescence Probes for Aminopeptidases: From Design to Biomedical Applications

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Biocompatible ratiometric fluorescent chemosensor for ultrasensitive detection of endogenous aminopeptidase N In Vitro and In Vivo

Cited by 7 publications

References 53 publications

Recent Advances of Aminopeptidases‐Responsive Small‐Molecular Probes for Bioimaging

Recent Advances of Aminopeptidases‐Responsive Small‐Molecular Probes for Bioimaging

Alkaline Phosphatase-Decorated Au Nanoparticles Immobilized on Bi2S3 Particles Combined with Ag2S Quantum Dots as a Photoelectrochemical Sensing Platform for Alkaline Phosphatase Activity and Fluoride Ions

Activatable Fluorescence and Bio/Chemiluminescence Probes for Aminopeptidases: From Design to Biomedical Applications

Contact Info

Product

Resources

About

Alkaline Phosphatase-Decorated Au Nanoparticles Immobilized on Bi₂S₃ Particles Combined with Ag₂S Quantum Dots as a Photoelectrochemical Sensing Platform for Alkaline Phosphatase Activity and Fluoride Ions