Biocomposites Electrospun with Poly(ε-caprolactone) and Silk Fibroin Powder for Biomedical Applications

Lee, Hyeongjin; Kim, GeunHyung

doi:10.1163/092050609x12548956645680

Cited by 24 publications

(12 citation statements)

References 21 publications

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“…27 However, mechanical properties of pure biopolymers are generally inferior to synthetic polymers, 25,28,29 although composite fibers of synthetic and biopolymers may have improved tensile properties compared to the synthetic polymer especially at low concentrations. [30][31][32] Physical methods have been used to improve the porosity and therefore cell infiltration, including the use of sacrificial fibers, which enhance cell infiltration and matrix deposition in vitro and in subcutaneous pouch models. 25,26 However, in an over-the-top rotator cuff augmentation animal model, this approach was less successful possibly because the scaffold with sacrificial fibers was less resistant to compression during handling and in a challenging in vivo environment.…”

Section: Introductionmentioning

confidence: 99%

Multilayered Electrospun Scaffolds for Tendon Tissue Engineering

Chainani

Hippensteel

Kishan

et al. 2013

Tissue Engineering Part A

103

115

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Full-thickness rotator cuff tears are one of the most common causes of shoulder pain in people over the age of 65. High retear rates and poor functional outcomes are common after surgical repair, and currently available extracellular matrix scaffold patches have limited abilities to enhance new tendon formation. In this regard, tissue-engineered scaffolds may provide a means to improve repair of rotator cuff tears. Electrospinning provides a versatile method for creating nanofibrous scaffolds with controlled architectures, but several challenges remain in its application to tissue engineering, such as cell infiltration through the full thickness of the scaffold as well as control of cell growth and differentiation. Previous studies have shown that ligament-derived extracellular matrix may enhance differentiation toward a tendon or ligament phenotype by human adipose stem cells (hASCs). In this study, we investigated the use of tendon-derived extracellular matrix (TDM)-coated electrospun multilayered scaffolds compared to fibronectin (FN) or phosphate-buffered saline (PBS) coating for use in rotator cuff tendon tissue engineering. Multilayered poly(ɛ-caprolactone) scaffolds were prepared by sequentially collecting electrospun layers onto the surface of a grounded saline solution into a single scaffold. Scaffolds were then coated with TDM, FN, or PBS and seeded with hASCs. Scaffolds were maintained without exogenous growth factors for 28 days in culture and evaluated for protein content (by immunofluorescence and biochemical assay), markers of tendon differentiation, and tensile mechanical properties. The collagen content was greatest by day 28 in TDM-scaffolds. Gene expression of type I collagen, decorin, and tenascin C increased over time, with no effect of scaffold coating. Sulfated glycosaminoglycan and dsDNA contents increased over time in culture, but there was no effect of scaffold coating. The Young's modulus did not change over time, but yield strain increased with time in culture. Histology demonstrated cell infiltration through the full thickness of all scaffolds and immunofluorescence demonstrated greater expression of type I, but not type III collagen through the full thickness of the scaffold in TDM-scaffolds compared to other treatment groups. Together, these data suggest that nonaligned multilayered electrospun scaffolds permit tenogenic differentiation by hASCs and that TDM may promote some aspects of this differentiation.

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Section: Introductionmentioning

confidence: 99%

Multilayered Electrospun Scaffolds for Tendon Tissue Engineering

Chainani

Hippensteel

Kishan

et al. 2013

Tissue Engineering Part A

103

115

View full text Add to dashboard Cite

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“…[76][77][78][79] Using this concept, extensive work on generating scaffolds from blends of natural and synthetic polymers [80][81][82][83] and other crystalline components has been performed. 84 After selecting suitable solvents, different polymers have been blended to control the mechanical and biological nature of the porous 24,88 ; for example, reduced pore size restricts cells and other particulates from infiltrating into the sub-layers accessing the 3D space.…”

Section: Materials For Electrospinningmentioning

confidence: 99%

Next Generation of Electrosprayed Fibers for Tissue Regeneration

Hong

Madihally

2011

Tissue Engineering Part B: Reviews

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Electrospinning is a widely established polymer-processing technology that allows generation of fibers (in nanometer to micrometer size) that can be collected to form nonwoven structures. By choosing suitable process parameters and appropriate solvent systems, fiber size can be controlled. Since the technology allows the possibility of tailoring the mechanical properties and biological properties, there has been a significant effort to adapt the technology in tissue regeneration and drug delivery. This review focuses on recent developments in adapting this technology for tissue regeneration applications. In particular, different configurations of nozzles and collector plates are summarized from the view of cell seeding and distribution. Further developments in obtaining thick layers of tissues and thin layered membranes are discussed. Recent advances in porous structure spatial architecture parameters such as pore size, fiber size, fiber stiffness, and matrix turnover are summarized. In addition, possibility of developing simple three-dimensional models using electrosprayed fibers that can be utilized in routine cell culture studies is described. Tissue EngineeringT issue engineering or regeneration is a multidisciplinary study to restore, maintain, and enhance tissue and organ function. 1 In tissue engineering, biodegradable scaffolds are used to support and guide cells to proliferate, organize, and produce their own extracellular matrix (ECM). Scaffolding material eventually disappears leaving only the necessary healthy tissue in a topologically required form. 2,3 Assembly and maturation of ECM elements is important in determining the biomechanics and the quality of the tissue. For example, collagen provides tensile strength to the tissue, elastic fibers contribute to the elasticity of the tissue, while proteoglycans fills the extracellular space, creating a space for the tissue regulation of growth factors and other interactions. 4 Delicate balance between different matrix elements is necessary to generate healthy tissue. The size and shape of collagen fibers in ECM relies on tissues and organs even in the same species. The shape is cord or tape with a width of 1-20 mm and the collagen fibrils (unit can be observed by electron microscopy) are cylindrical with a diameter ranging from 10 to over 500 nm where cell is attaching and hugging. 5 Obtaining a biodegradable matrix conducive for cell colonization is a fundamental requirement for tissue regeneration. Like ECM, biomimic scaffold should allow cell attachment and migration, enables diffusion of vital cell nutrients, and retains cells. Further, chemical and mechanical properties of scaffold influence cell viability and proliferation. 6 Appropriate pore size and porosity are essential to modulate cell seeding and diffusion. Biodegradability is essential because scaffolds are absorbed and distributed to the nearby tissue, which allows no surgical removal from body. The surface of scaffold is suitable for cell attachment and migration. The mechanical properties...

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“…The bioactive proteins have been successfully incorporated into nanofibers to improve interactions between the PCLbased substrate and cells. Previous works have produced PCL nanofibrous scaffolds with mulberry silk fibroin by using blend (Lee and Kim 2010) and emulsion electrospinning (Li et al 2011). No similar work has been carried out on incorporating PCL nanofibrous scaffolds with non-mulberry tropical tasar silk protein fibroin.…”

Section: Introductionmentioning

confidence: 99%

Potential of inherent RGD containing silk fibroin–poly (Є-caprolactone) nanofibrous matrix for bone tissue engineering

et al. 2015

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The current study deals with the fabrication and characterization of blended nanofibrous scaffolds of tropical tasar silk fibroin of Antheraea mylitta and poly (Є-caprolactone) to act as an ideal scaffold for bone regeneration. The use of poly (Є-caprolactone) in osteogenesis is well-recognized. At the same time, the osteoconductive nature of the non-mulberry tasar fibroin is also established due to its internal integrin binding peptide RGD (Arg-Gly-Asp) sequences, which enhance cellular interaction and proliferation. Considering that the materials have the required and favorable properties, the blends are formed using an equal volume ratio of fibroin (2 and 4 wt%) and poly (Є-caprolactone) solution (10 wt%) to fabricate nanofibers. The nanofibers possess an average diameter of 152 ± 18 nm (2 % fibroin/PCL) and 175 ± 15 nm (4% fibroin/PCL). The results of Fourier transform infrared spectroscopy substantiates the preservation of the secondary structure of the fibroin in the blends indicating the structural stability of the neo-matrix. With an increase in the fibroin percentage, the hydrophobicity and thermal stability of the matrices as measured from melting temperature Tm (using DSC) decrease, while the mechanical strength is improved. The blended nanofibrous scaffolds are biodegradable, and support the viability and proliferation of human osteoblast-like cells as observed through scanning electron and confocal microscopes. Alkaline phosphatase assay indicates the cell proliferation and the generation of the neo-bone matrix. Taken together, these findings illustrate that the silk-poly (Є-caprolactone) blended nanofibrous scaffolds have an excellent prospect as scaffolding material in bone tissue engineering.

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Biocomposites Electrospun with Poly(ε-caprolactone) and Silk Fibroin Powder for Biomedical Applications

Cited by 24 publications

References 21 publications

Multilayered Electrospun Scaffolds for Tendon Tissue Engineering

Multilayered Electrospun Scaffolds for Tendon Tissue Engineering

Next Generation of Electrosprayed Fibers for Tissue Regeneration

Potential of inherent RGD containing silk fibroin–poly (Є-caprolactone) nanofibrous matrix for bone tissue engineering

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