Bioconcentration Assessment of Three Cationic Surfactants in Permanent Fish Cell Lines

Abstract

Cationic surfactants are used in many industrial processes and in consumer products with concurrent release into the aquatic environment, where they may accumulate in aquatic organisms to regulatoryly relevant thresholds. Here, we aimed to better understand the bioconcentration behavior of three selected cationic surfactants, namely N,N-dimethyldecylamine (T10), N-methyldodecylamine (S12), and N,N,N-trimethyltetradecylammonium cation (Q14), in the cells of fish liver (RTL-W1) and gill (RTgill-W1) cell lines. We conducted full mass balances for bioconcentration tests with the cell cultures, in which the medium, the cell surface, the cells themselves, and the plastic compartment were sampled and quantified for each surfactant by HPLC MS/MS. Accumulation in/to cells correlated with the surfactants' alkyl chain lengths and their membrane lipid−water partitioning coefficient, D MLW . Cell-derived bioconcentration factors (BCF) of T10 and S12 were within a factor of 3.5 to in vivo BCF obtained from the literature, while the cell-derived BCF values for Q14 were >100 times higher than the in vivo BCF. From our experiments, rainbow trout cell lines appear as a suitable conservative in vitro screening method for bioconcentration assessment of cationic surfactants and are promising for further testing.

A Visible-Light-Driven Self-Powered Nodularin-R Biosensing Platform Controlled by an Integrated Portable Photoelectrochemical Detection Device

“…Several analytical techniques have been developed for the detection of NOD-R, including enzyme-linked immunosorbent assays, liquid chromatography-tandem mass spectrometry (LC-MS/MS), and fluorescence polarization immunoassays . However, these methods often involve excessive use of harmful reagents, expensive instrumentation, and require specialized operators, falling short of the requirements for on-site detection. − Recently, there has been increasing interest in the use of photoelectrochemical (PEC) techniques for monitoring water-related contaminants owing to their simplicity, rapidity, and high sensitivity. , Ouyang et al introduced a self-powered PEC sensor that relied on xenon lamp excitation and achieved highly sensitive microcystin-LR (MC-LR) detection by constructing Au/CeO 2 /g-C 3 N 4 heterostructures.…”