Bioconjugated Fluorescent Zeolite L Nanocrystals as Labels in Protein Microarrays

Abstract

Zeolite L nanocrystals, as inorganic host material containing hydrophobic fluorophore N,N'-bis(2,6-dimethylphenyl)perylene-3,4,9,10-tetracarboxylic diimide in the unidirectional channels, are developed as new labels for biosensor systems. The external surface of the particles is modified with carboxylic acid groups for conjugation to primary amines of biomolecules such as antibodies. Anti-digoxigenin (anti-DIG) is selected to be immobilized on zeolite L via N-hydroxysulfosuccinimide ester linker. Together with DIG, it serves as a good universal binding pair for diverse analyte detection owing to the high binding affinity and low background noise. The conjugates are characterized by the dynamic light scattering technique for their hydrodynamic diameters and by enzyme-linked immunosorbent assay for antigen-antibody binding behavior. The characterizations prove that anti-DIG antibodies are successfully immobilized on zeolite L with their binding activities maintained. The microarray fluorescent sandwich immunoassay based on such nanocrystalline labels shows high sensitivity in a thyroid-stimulating hormone assay with the lower detection limit down to the femtomolar range. These new fluorescent labels possess great potential for in vitro diagnostics applications.

Close-Packed Dye Molecules in Zeolite Channels Self-Assemble into Supramolecular Nanoladders

“…1,2,3 Microlasers, pigments, optical switches, or artificial antenna systems are only few of the possible applications of these fascinating systems. 4,5,6,7,8,9,10 To date, different zeolites with suitable channel dimensions, such as AlPO4-5, 11,12 zeolite Y, 13 zeolite L, 14,15 as well as mesoporous materials like MCM-41, 16 have been successfully adopted as nanosized host matrices for the synthesis of these composites. In this rapidly evolving scenario, the inclusion of photoactive molecules into one-dimensional channel systems is of paramount relevance for further progress in some of the most challenging fields of nanoscience and nanotechnology.…”

First-principles simulation of the absorption bands of fluorenone in zeolite L

“…[3][4][5][6][7][8][9][10][11][12][13][14][15][16][17][18] These are currently investigated for applications in different fields, ranging from sensing in analytical chemistry, biology, and diagnostics, [15][16][17][18] to applications in optical and electro optical devices [3][4][5][19][20][21] and extending to solar energy utilization, e.g. in the form of luminescent solar concentrators.…”

Internalization Pathways of Anisotropic Disc‐Shaped Zeolite L Nanocrystals with Different Surface Properties in HeLa Cancer Cells

“…Disc‐shaped zeolite L nanocrystals were synthesized via a hydrothermal process as reported in the literature 24. The X‐ray diffraction pattern show (Figure S1) that the obtained crystals have the complete structure of zeolite L nanocrystals.…”

“…Among them, 200 nm disc‐shaped particles have the advantage that they have fewer tendencies to form aggregates in suspension. Loaded with highly fluorescent molecules, such nanoparticles have shown great potential in immunoassays 24…”