Bioconjugated quantum dots for cancer research: Present status, prospects and remaining issues

Biju, Vasudevanpillai; Mundayoor, Sathish; Omkumar, Ramakrishnapillai V.; Anas, Abdulaziz; Ishikawa, Mitsuru

doi:10.1016/j.biotechadv.2009.11.007

Cited by 214 publications

(74 citation statements)

References 120 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…This finding agrees with Derfus et al [15] who reported that Cd-based QDs released toxic levels of cadmium ions inside cells resulting in cell death [3,15]. In addition, Cho et al [27] found that mercaptoacetic acid-capped CdSe caused severe mitochondrial impairment and cell death due to the production of free radicals and simultaneous release of cadmium ions [3,27]. In our case, the physiological and pH conditions inside the cells would have promoted the degradation of the TGA coating and subsequent chemical dissolution of cadmium species from the QDs.…”

Section: Panc-1 Viability In Presence Of Qdssupporting

confidence: 92%

“…This decrease of the Cd/Se molar ratio could suggest some selective release of Cd species out from the QDs after ingestion by PANC-1. This finding agrees with Derfus et al [15] who reported that Cd-based QDs released toxic levels of cadmium ions inside cells resulting in cell death [3,15]. In addition, Cho et al [27] found that mercaptoacetic acid-capped CdSe caused severe mitochondrial impairment and cell death due to the production of free radicals and simultaneous release of cadmium ions [3,27].…”

Section: Panc-1 Viability In Presence Of Qdssupporting

confidence: 87%

“…QDs typically consist of II-VI or III-V semiconductor crystals in the 2-12 nm size range, which exhibit unique electronic and optical properties. Their broad absorption spectra, narrow bandwidth emission, high-quantum yield, resistance to photobleaching and the capability to generate toxic species such as singlet oxygen, make them very attractive materials for nanomedicine applications [3].…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

UV-enhanced toxicity of water-stable quantum dots in human pancreatic carcinoma cells

Bailón-Ruiz

Perales-Pérez

2013

Journal of Experimental Nanoscience

View full text Add to dashboard Cite

Zn-based (capped with thioglycolic acid (TGA) or 3-mercaptopropionic acid (MPA)) and Cd-based quantum dots (QDs) (capped with TGA or L-glutathione), were synthesised and used to investigate their cytotoxicity to human pancreatic carcinoma cells (PANC-1) in absence and presence of UV irradiation. Zn-based QDs exhibited less intrinsic cytotoxicity than the Cd-based QDs, however, the excitation of 50 mg/mL-QDs using UV lamp significantly enhanced the cytotoxicity of both QDs. After 15 min of UV irradiation, the viability for cells exposed to Cd-based QDs capped with TGA or glutathione was 49% AE 6% or 56% AE 3%, respectively. The corresponding cell viability in the control test was 83% AE 8% after 15 min of UV irradiation. In turn, the viability for cells exposed to Zn-based QDs capped with 3-MPA or TGA was 64% AE 3% and 52% AE 3%, respectively, after 30 min of UV irradiation; the cell viability in the control test was 80% AE 7% for the same UV irradiation time. Laser scanning confocal analyses evidenced that QDs can be easily ingested by PANC-1. Based on their good compositional stability, Zn-based QDs capped with 3-MPA can be considered a promising material for nanomedicine applications until concentrations of 200 mg/mL.

show abstract

Section: Panc-1 Viability In Presence Of Qdssupporting

confidence: 92%

Section: Panc-1 Viability In Presence Of Qdssupporting

confidence: 87%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

UV-enhanced toxicity of water-stable quantum dots in human pancreatic carcinoma cells

Bailón-Ruiz

Perales-Pérez

2013

Journal of Experimental Nanoscience

View full text Add to dashboard Cite

show abstract

“…21,22 Besides imaging, QDs are now emerging as therapeutic agents alone 23 or in combination with external radiation. [24][25][26] It has been recently shown that QDs irradiated with UV radiation, [27][28][29] blue light, photodynamic application. The most likely mechanism of photosensitized action of QDs is photogeneration of excitons and electron-hole pairs (e − and h + ) 32 and subsequent electron transfer to oxygen adsorbed on the QD surface forming a superoxide (O 2 − ) free radical.…”

Section: Introductionmentioning

confidence: 99%

Entrapment in phospholipid vesicles quenches photoactivity of quantum dots

Generalov

Kavaliauskiene²,

Westrøm³

et al. 2011

IJN

View full text Add to dashboard Cite

Quantum dots have emerged with great promise for biological applications as fluorescent markers for immunostaining, labels for intracellular trafficking, and photosensitizers for photodynamic therapy. However, upon entry into a cell, quantum dots are trapped and their fluorescence is quenched in endocytic vesicles such as endosomes and lysosomes. In this study, the photophysical properties of quantum dots were investigated in liposomes as an in vitro vesicle model. Entrapment of quantum dots in liposomes decreases their fluorescence lifetime and intensity. Generation of free radicals by liposomal quantum dots is inhibited compared to that of free quantum dots. Nevertheless, quantum dot fluorescence lifetime and intensity increases due to photolysis of liposomes during irradiation. In addition, protein adsorption on the quantum dot surface and the acidic environment of vesicles also lead to quenching of quantum dot fluorescence, which reappears during irradiation. In conclusion, the in vitro model of phospholipid vesicles has demonstrated that those quantum dots that are fated to be entrapped in endocytic vesicles lose their fluorescence and ability to act as photosensitizers.

show abstract

“…Some of the main application areas include nucleic acid detection [32][33][34][35] , immunoassays [36][37][38] , cell tracking, tumor targeting and fluorescence resonance energy transfer (FRET) sensing [39][40][41][42] . However, toxicity of QDs remains a concern for invivo applications 43,44) .…”

Section: Synthesismentioning

confidence: 99%