Bioconjugates of photon-upconversion nanoparticles for cancer biomarker detection and imaging

Hlaváček, Antonín; Farka, Zdeněk; Mickert, Matthias J.; Kostiv, Uliana; Brandmeier, Julian C.; Horák, Daniel; Skládal, Petr; Foret, František; Gorris, Hans H.

doi:10.1038/s41596-021-00670-7

Cited by 95 publications

(65 citation statements)

References 124 publications

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“…The NaYF 4 lanthanide-doped UCNPs with a core-only or core/inert-shell architecture were synthesized in high-boiling-point solvents. 13 These nanoparticles were coated with hydrophilic carboxylated silica to prepare aqueous dispersions. 14−17 A sample of Er 3+ -doped UCNPs (UCNP_Er) coated in carboxylated silica was used for preparing the UCNP−antibody conjugate by a carbodiimide method.…”

Section: ■ Introductionmentioning

confidence: 99%

Absolute Counting Method with Multiplexing Capability for Estimating the Number Concentration of Nanoparticles Using Anisotropically Collapsed Gels

et al. 2022

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Number concentrationthe number of nanoparticles in a given volumeis an important characteristic of any nanoparticle dispersion. However, its estimation for small nanoparticles (∼30 nm) is generally challenging. We introduce an absolute and widely applicable method for analyzing aqueous dispersions of nanoparticles. An innovative immobilization of nanomaterials in the anisotropically collapsed agarose gel is pioneered, followed by optical microscopy and nanoparticle counting. The number of counted nanoparticles is inherently coupled with sampled volume (517 pL) and translates to the number concentration. Photon-upconversion, fluorescence, bright-field, and dark-field microscopy techniques have been proven applicable and used for imaging lanthanide-doped photon-upconversion nanoparticles, their bioconjugates with antibodies, silica dye-doped fluorescent nanoparticles, quantum dots, and pure silica submicron particles. The precision and linearity were characterized by constructing a dilution series of photon-upconversion nanoparticles. The limit of detection was 2.0 × 106 mL–1, and the working range was from 4.4 × 107 to 2.2 × 1010 mL–1. The quantification of nanoparticle clusters was achieved by a thorough analysis of the micrographs. The accuracy was confirmed using gravimetric analysis and transmission electron microscopy as a reference. Multiplexed detection of two nanoparticle types in a mixed dispersion was feasibly demonstrated. The low thickness of the collapsed gel (<1 μm) supported extremely sensitive imaging. This was proven by imaging Tm3+-doped photon-upconversion nanoparticles (17 nm hydrodynamic diameter) with a nanoparticle emission rate of only ∼900 photons/s at a wavelength of 800 nm (excitation wavelength 976 nm).

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Section: ■ Introductionmentioning

confidence: 99%

Absolute Counting Method with Multiplexing Capability for Estimating the Number Concentration of Nanoparticles Using Anisotropically Collapsed Gels

et al. 2022

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“…important intermediate step for further ligand modification. 37 It is worth noting that ligand free, as a general method of surface engineering, is an important preprocessing for further surface modification before biological applications. In general, it is difficult to completely replace the original oleate by a new ligand, even if the ligand is functionalized with phosphate.…”

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“…In addition, the PEG ligand can be easily functionalized with bioconjugated groups such as alkyne, so as to realize the specific analysis of special biomarkers by utilizing sophisticated analytical method such as immunocytochemistry analysis. 37,39 In particular, Gorris recently has shared a protocol that achieves bioconjugation of LnNPs for cancer biomarker detection and imaging. 37 By using the ligand exchange engineered LnNPs, they achieved the detection of breast cancer markers with a 50-fold improved signal-to-noise ratio compared to that of the conventional fluorescent label in immunocytochemistry analysis.…”

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“…37,39 In particular, Gorris recently has shared a protocol that achieves bioconjugation of LnNPs for cancer biomarker detection and imaging. 37 By using the ligand exchange engineered LnNPs, they achieved the detection of breast cancer markers with a 50-fold improved signal-to-noise ratio compared to that of the conventional fluorescent label in immunocytochemistry analysis. This is undoubtedly a breakthrough in both nanomedicine and cancer diagnosis.…”

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Ligand-Based Surface Engineering of Lanthanide Nanoparticles for Bioapplications

Zhu

Zhang

2022

ACS Materials Lett.

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The rapid development of biomedicine in recent decades has benefited from optical imaging with high temporal and spatial resolution. In particular, lanthanide nanoparticles (LnNPs) are one of the most impressive signal providers for optical imaging due to their unique optical properties, such as abundant emission peaks, long luminescence lifetimes, and ultrastable luminescence intensity. However, to become better optical labels that are more suitable for biological applications, LnNPs must overcome their inherent flaws, including original hydrophobic surface, insufficient luminescence intensity, as well as the low environmental responsiveness. Recently, researchers have achieved extraordinary results in ameliorating these defects through ligand-based surface engineering. In this Review, we discussed several representative methods for the hydrophilic modification of LnNPs. Then, the significant attempts for luminescence amplification of LnNPs were concluded. Subsequently, attentions were paid to the methods that helped to fabricate responsive LnNPs systems, as well as the potential biomedical applications. Finally, we considered the current limitations and prospects of the surface engineering strategies for LnNPs. We hope this timely Review can exhibit the outstanding achievement in manipulating the surface properties of LnNPs and promote a broader development of LnNPs in the field of biomedical applications.

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“…This dual-mode luminescent nanocomposite provides a promising blueprint for the design of a wide range of heterostructures with tailor-made optical properties. In addition to information storage and anticounterfeiting, these nanocomposites may be particularly useful for multiplexed applications in diagnostics and imaging 6 . Further applications will strongly benefit from improving the quantum yields both in the upconversion mode (<1%) and in the down-shifting mode (5%).…”

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Up and down the spectrum: upconversion nanocrystal and semiconductor material fused into a single nanocomposite

Gorris

2022

Light Sci Appl

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A nanocomposite consisting of a cubic EuSe semiconductor material grown on a hexagonal upconversion nanoparticle has overcome the crystal lattice mismatch that typically prevents the epitaxial growth of such heterogeneous nanocrystals. Eu3+ at the interface layer shows its characteristic red emission band both under UV excitation light due to energy transfer from the semiconductor and under NIR excitation light due to energy transfer after photon-upconversion. Data storage and security applications are suggested for this new nanocomposite.

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Bioconjugates of photon-upconversion nanoparticles for cancer biomarker detection and imaging

Cited by 95 publications

References 124 publications

Absolute Counting Method with Multiplexing Capability for Estimating the Number Concentration of Nanoparticles Using Anisotropically Collapsed Gels

Absolute Counting Method with Multiplexing Capability for Estimating the Number Concentration of Nanoparticles Using Anisotropically Collapsed Gels

Ligand-Based Surface Engineering of Lanthanide Nanoparticles for Bioapplications

Up and down the spectrum: upconversion nanocrystal and semiconductor material fused into a single nanocomposite

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