Bioconjugation of Interferon-alpha Molecules to Lysine-Capped Gold Nanoparticles for Further Drug Delivery Applications

Aghdam, Atefeh Ghorbani; Vossoughi, M.; Almzadeh, Iran; Zeinali, Majid

doi:10.1080/01932690701815762

Cited by 13 publications

(11 citation statements)

References 28 publications

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“…EDAC forms an unstable intermediate complex with carboxylic groups, which is susceptible to amide bond formation if it reacts with a primary amine. A recent example of EDAC use in bioconjugation can be found in the work of Aghdam and coworkers, who immobilized the N-terminus of lysine on gold nanoparticles and proceeded to bind interferons to the free C-termini [12].…”

Section: Introductionmentioning

confidence: 99%

Bridging flocculation of PEI-functionalized latex particles using nanocrystalline cellulose

Manfredi

Hill

Ven

2011

Journal of Colloid and Interface Science

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Section: Introductionmentioning

confidence: 99%

Bridging flocculation of PEI-functionalized latex particles using nanocrystalline cellulose

Manfredi

Hill

Ven

2011

Journal of Colloid and Interface Science

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“…'AuNPs' is the area of research interest due to their unique properties such as tunable surface plasmon resonance (SPR), surface-enhanced Raman scattering, electrical, magnetic, thermal conductivity, antibacterial activity, chemical and biostability [7][8][9][10][11][12][13]. Besides this, the use of AuNPs as potential materials in the field of drug delivery and DNA delivery systems is noteworthy [14,15]. Most of the available methods for the synthesis of AuNPs involve photochemical reduction, chemical reduction, and electrochemical reduction [16][17][18].…”

Section: Introductionmentioning

confidence: 99%

Green chemistry approach for the synthesis of gold nanoparticles with gum kondagogu: characterization, catalytic and antibacterial activity

et al. 2015

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Gold nanoparticles (AuNPs) were prepared from HAuCl 4 using gum kondagogu, by adopting green synthesis, which is a simple, low cost and ecofriendly technique. The gum kondagogu (Cochlospermum gossypium) serves as both reducing agent and stabilizer. The formation of the AuNPs was identified through the change in the color of the solution from yellow to red. The synthesized AuNPs were characterized by various techniques. The green synthesized AuNPs were found to be stable in the pH range pH 2-12 and up to the concentration of 5 M NaCl. The stabilized AuNPs demonstrated the excellent catalytic activity in reducing p-nitrophenol to p-aminophenol in the presence of a reducing agent, NaBH 4 . The effects of catalyst dose and temperature were studied. The synthesized, new gum-based catalyst was very efficient, easy to prepare, stable, cost-effective and ecofriendly. The synthesized AuNPs showed good antibacterial activity.

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“…The use of amine-terminating ligands for binding with GNPs is well established [68][69][70][71] although not very popular with glycans yet. Indeed, the direct conjugation of amino-functionalized antigenic glycans with pre-formed GNPs has not earlier been reported to the best our knowledge although simple amines, amino acids and peptides [68][69][70][71][72][73][74][75][76][77][78], as well as diethylaminoethyl-dextran [79] and chitosan [80] have been reported to bind to GNPs in pH-dependent manner as the energy of Au-N interaction is intermediate between those of Au-S and Au-O [81] (the affinity of different functional groups to the GNPs's surface decreases in the series Au-S>Au-NH2>Au-COOH [82]) thus making exchange of citrate ligands with amines possible. The amine-capped GNPs are stable enough to be used as targeting agents for drug delivery applications [75], antigens for generation of antibodies [77] or antimicrobial agents [78].…”

Section: Tuberculosis (Tb) Which Is Caused By the Pathogenic Bacterimentioning

confidence: 99%

Preparation of glyco-gold nanoparticles carrying synthetic mycobacterial hexaarabinofuranoside and their evaluation in vivo

Burygin¹,

Abronina²,

Podvalnyy³

et al. 2019

Preprint

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Some bacterial glycans are specific markers for detection and serological identification of microorganisms and are widely used as antigenic components of vaccines. The use of gold nanoparticles as carriers for glyco-epitopes is becoming an important alternative to the traditional conjugation with proteins and synthetic polymers. In this study, we aimed at preparation and evaluation in vivo of glyco-gold nanoparticles (glyco-GNPs) bearing the terminal branched hexaarabinofuranoside fragment (Ara6) of arabinan domains of lipoarabinomannan and arabinogalactan, which are principal polysaccharides of the cell wall of Mycobacterium tuberculosis, the causative agent of tuberculosis. Specifically, we were interested in checking if the antibodies generated against Ara6-GNPs would recognize the natural saccharides on the cell surface of different mycobacterial strains. Two synthetic Ara6 glycosides with amino-functionalized spacer aglycons differing in length and hydrophilicity were directly conjugated with spherical gold nanoparticles (d = 15 nm) to give two sets of glyco-GNPs which were used for immunization of rabbits. Dot assay revealed cross-reactions for the two obtained antisera with the hexaarabinofuranoside with 2-aminoethyl aglycon used for the preparation of glyco-GNPs. Both antisera contained high titers of antibodies specific for Mycobacteria as shown by enzyme-linked immunosorbent assay using M. bovis and M. smegmatis cells as antigens while gave a weak response with M. phlei cells and no interaction with E. coli cells. The results obtained suggest that glyco-GNPs are promising agents for generation of anti-mycobacterial antibodies.

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Bioconjugation of Interferon-alpha Molecules to Lysine-Capped Gold Nanoparticles for Further Drug Delivery Applications

Cited by 13 publications

References 28 publications

Bridging flocculation of PEI-functionalized latex particles using nanocrystalline cellulose

Bridging flocculation of PEI-functionalized latex particles using nanocrystalline cellulose

Green chemistry approach for the synthesis of gold nanoparticles with gum kondagogu: characterization, catalytic and antibacterial activity

Preparation of glyco-gold nanoparticles carrying synthetic mycobacterial hexaarabinofuranoside and their evaluation in vivo

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