Bioconjugation with Stable Luminescent Lanthanide(III) Chelates Comprising Pyridine Subunits

Abstract

The use of long-lifetime emitting lanthanide(III) chelate labels or probes together with time-resolved fluorometry in detection provides a method to generate sensitive bioaffinity assays. However, the use of stable chelates demands very complicated optimization of the chelate structure. A great number of chelates have been synthesized, but usually, only the most prominent structures were then converted to corresponding biomolecule labeling reactants. This review covers the syntheses of luminescent lanthanide chelates comprising a pyridine subunit that allow solution and solid-phase bioconjugation.

Thermodynamic study of Eu(III) complexation by pyridine monocarboxylates

“…Lanthanide complexes containing organic ligand with conjugate group have been widely studied because of their characteristic luminescent properties, which are applied to biomedical, sensing, industrial and bioanalytical applications [1][2][3] and serve as lightemitting materials in optical devices [4][5][6][7]. Pyridine monocarboxylates, such as, picolinate, nicotinate and isonicotinate have been widely used to build lanthanide complexes and coordination networks [8] because those molecules with nitrogen and oxygen atoms could efficiently transfer energy to lanthanide ion [9][10][11][12][13][14].…”

Group 3 Elements and Lanthanide Metals

“…YAG:Ce bridges the "yellow gap" of semiconductor primary LED phosphors as well as that it can be used in laser applications and moreover doped with other lanthanide ions like Nd 3+ or Er 3+ [173][174][175]. New bioapplications in medical and biological analysis, like monitoring drug delivery [176][177][178][179], have attracted attention as luminescent lanthanide coordination compounds like -diketonates can be used in time-resolved luminescent (TRL) immunoassays and Ln-MOFs for various imaging techniques including magnetic resonance imaging (MRI) [180][181][182]. Lanthanide coordination and framework compounds have also started to influence lighting in OLED technologies [183][184][185][186][187].…”

Photophysical studies of highly luminescent europium(III) and terbium(III) complexes functionalized with amino and mercapto groups

“…[5]. For biological probes applications it is desirable a high quantum yield and also the presence of non-coordinated functional groups such as amino (-NH 2 ), mercapto (-SH), carboxylate (-COO À ) and others that may be used for interactions with a target, such as a biomolecule [6], silver or gold nanoparticles [7][8][9] or silica networks [10,11]. The interaction of functionalized-lanthanide complexes with silver or gold nanoparticles is interesting because the possibility of their emission intensity enhancement that may happen due to the interaction with the surface plasmon resonance of these nanoparticles [7].…”