2016
DOI: 10.15171/ijb.1224
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Biocontrol Activities of Gamma Induced Mutants of Trichoderma harzianum against some Soilborne Fungal Pathogens and their DNA Fingerprinting

Abstract: Background Random induced mutation by gamma radiation is one of the genetic manipulation strategies to improve the antagonistic ability of biocontrol agents. Objectives This study aimed to induce mutants with more sporulation, colonization rate leading to enhanced antagonistic ability (in vitro assay) comparing to wild type (WT) and the assessment of genetic differences (in situ evaluation) using molecular markers. The superior mutants could be appropriate biocontrol agents against soil borne fungal diseases. … Show more

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Cited by 30 publications
(17 citation statements)
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“…These obtained results was confirmed by the results of (Abbasi et al, 2016) who mentioned that gamma rays have very high energy, causing gene mutations by replacement of nucleotides (by oxidative deamination) or chromosome breakage. This was clear in this study as different doses of gamma radiation affect both physiological and molecular treats of the selected fungi.…”
Section: Discussionsupporting
confidence: 73%
“…These obtained results was confirmed by the results of (Abbasi et al, 2016) who mentioned that gamma rays have very high energy, causing gene mutations by replacement of nucleotides (by oxidative deamination) or chromosome breakage. This was clear in this study as different doses of gamma radiation affect both physiological and molecular treats of the selected fungi.…”
Section: Discussionsupporting
confidence: 73%
“…Hence, S. sclerotiorum exposed to mutants of T. harzianum after 3 days incubation in dual culture presented an inhibition over 70% for almost tested strains, with the highest value of 88% inhibition. The values for F. graminearum did not exceed 60%, the pathogen being more resistant to biocontrol agent [38]. A Trichoderma isolate showed 70% (R. solani), 68.2% (Sclerotinia rolfsii), 73.3% (M. phaseolina) and, 69.3% (Fusarium sp.)…”
Section: Discussionmentioning
confidence: 90%
“…Understanding the paths is of particular importance in molecular biology (31). If these paths are related to pathogenesis, they are more important (32)(33)(34)(35)(36). Medicinal plants have been reported to be very effective in this regard (37,38).…”
Section: Discussionmentioning
confidence: 99%