Biocontrol Activity of Bacillus amyloliquefaciens CNU114001 against Fungal Plant Diseases

Abstract

A total of 62 bacterial isolates were obtained from Gomsohang mud flat, Mohang mud flat, and Jeju Island, Republic of Korea. Among them, the isolate CNU114001 showed significant antagonistic activity against pathogenic fungi by dual culture method. The isolate CNU114001 was identified as Bacillus amyloliquefaciens by morphological observation and molecular data analysis, including 16SrDNA and gyraseA (gyrA) gene sequences. Antifungal substances of the isolate were extracted and purified by silica gel column chromatography, thin layer chromatography, and high performance liquid chromatography. The heat and UV ray stable compound was identified as iturin, a lipopeptide (LP). The isolate CNU114001 showed broad spectrum activity against 12 phytopathogenic fungi by dual culture method. The semi purified compound significantly inhibits the mycelial growth of pathogenic fungi (Alternaria panax, Botrytis cinera, Colletotrichum orbiculare, Penicillium digitatum, Pyricularia grisea and Sclerotinia sclerotiorum) at 200 ppm concentration. Spore germ tube elongation of Botrytis cinerea was inhibited by culture filtrate of the isolate. Crude antifungal substance showed antagonistic activity against cucumber scleotiorum rot in laboratory, and showed antagonistic activity against tomato gray mold, cucumber, and pumpkin powdery mildew in greenhouse condition.

Bacillus amyloliquefaciens subsp. plantarum GR53, a potent biocontrol agent resists Rhizoctonia disease on Chinese cabbage through hormonal and antioxidants regulation

“…GR53 was identified as B. amyloliquefaciens through phylogenetic analysis. Previous studies have also documented the ability of B. amyloliquefaciens to inhibit R. solani fungal growth (Chowdhury et al 2013;Huang et al 2012;Ji et al 2013;Yu et al 2002) via the production of antifungal substance iturin. Overall, B. amyloliquefaciens has been studied extensively as a producer of a-amylase, subtilisin, barnase, and iturins, all of which can control pathogen growth (Yu et al 2002).…”

“…In fact, B. amyloliquefaciens mediated suppression rates of Phytophthora-blight of peppers and Fusarium-wilt of tomatoes were found to be higher than those of popular chemical fungicides (Chung and Kim 2005). Ji et al (2013) demonstrated that B. amyloliquefaciens CNU114001 exhibited broad spectrum inhibitory activity against 12 phytopathogenic fungi such as Alternaria panax, Botrytis cinerea, Colletotrichum acutatum, Colletotrichum orbiculare, Corynespora cassiicola, Fusarium oxysporum, Phytophthora capsici, Penicillium digitatum, R. solani, Stemphylium lycopersici, Pyricularia grisea, and Sclerotinia sclerotiorum. Bacterial antibiotics control fungal growth by inhibiting the synthesis of fungal sterols and nucleic acids, as well as changing cell membrane permeability and destroying the fungal cell wall (Michael and Nannette 2003).…”

Biocontrol Potentials of Antimicrobial Peptide Producing Bacillus Species: Multifaceted Antagonists for the Management of Stem Rot of Carnation Caused by Sclerotinia sclerotiorum

“…Production of antimicrobial metabolites determines the ability of these species to control plant diseases. Crude extracts of B. amyloliquefaciens have been reported to inhibit mycelial growth and sclerotial formation in S. sclerotiorum (Ji, 2013). Similarly, in the present study with B. amyloliquefaciens (VB7 and VB2), ethyl acetate extracts of crude metabolites reduced mycelial growth.…”

Isolation and characterization of Bacillus altitudinis JSCX-1 as a new potential biocontrol agent against Phytophthora sojae in soybean [Glycine max (L.) Merr.]

“…Thus, Bacillus has a broad spectrum of activity against multiple fungal pathogens. Lipopeptides extracted from Bacillus amyloliquefaciens CNU114001 inhibited the mycelial growth of six pathogenic fungi and the elongation of spore germ tubes (Ji et al 2013). In addition, Bacillus releases volatile compounds, belonging to alkyls, alcohols, esters, ketones, phenols and heterocyclics, to suppress the mycelial growth and the spore germination of fungal pathogens such as Fusarium oxysporum, Fusarium solani, Sclerotinia sclerotiorum and Botrytis cinerea (Fiddaman and Rossall 1994;Yuan et al 2012;Li et al 2014).…”