Biocontrol and growth enhancement potential of Trichoderma spp. against Rhizoctonia solani causing sheath blight disease in rice

Abstract

The present study aimed to investigate the biocontrol and growth enhancement potentials of Trichoderma spp., against R. solani. A total of 31 Trichoderma spp. were examined for their antagonistic potentials against R. solani in dual culture, effect of volatile and nonvolatile metabolites on the growth inhibition of test pathogen under in-vitro condition. The efficacy of Trichoderma spp. were further evaluated for controlling sheath blight and promoting rice growth under in-vivo conditions with different mode of applications. The results of in-vitro confrontation assay revealed that among the different Trichoderma spp. tested, T. harzianum (SVPRT-THLi06) and T. atroviride (SVPP-4) exhibited excellent biocontrol efficacy with 90.9% and 72.0% mycelial growth inhibition, respectively. The culture filtrate of T. harzianum (SVPRT-THLi6) at 50% was highly effective in reducing R. solani mycelial growth up to 95.9% and in case of volatile metabolites, maximum inhibition (72.5%) in mycelial growth of R. solani was recorded with T. harzianum isolate SVPP-8. A combined mode of application (soil treatment+ root dipping+ foliar spray) with T. harzianum (SVPRT-THLi06) was found most effective under greenhouse condition, which showed the least disease severity (12.4% vs control 49.6%) and disease incidence (27.1% vs control 96.4%). The present study revealed that T. harzianum (SVPRT-THLi06) has potential to control sheath blight disease as well as improve growth of rice crop. Therefore, in future it may be used as bio-based formulation for plant growth enhancement and managing sheath blight disease in rice cultivation and further may be applied in organic rice cultivation. Necrotrophic fungus, Rhizoctonia solani, Rice, Sheath blight, Trichoderma spp. Biocontrol and growth enhancement potential of Trichoderma spp. against Rhizoctonia solani causing sheath blight disease in rice

Biocontrol of Phytophthora xcambivora on Castanea sativa: Selection of Local Trichoderma spp. Isolates for the Management of Ink Disease

“…These features represent a very important characteristic to be evaluated in several species of Trichoderma used as beneficial fungi against several plant pathogens, in both forest and crop patho-systems. In particular, T. harzianum, T. atroviride, T. virens, T. koningiopsis, T. hamatum and T. asperellum were reported to overgrow and sporulate on the target pathogen in dual cultures, the latter considered as a sign of mycoparasitism [21,27,[68][69][70][71].…”

Determination of antifungal efficiency of some fungicides and secondary metabolites of Trichoderma species against Botrytis cinerea

“…The volatile metabolites secreted by the T. atroviride strain were found to have the highest inhibition rates against Fusarium graminearum, Rhizoctonia solani and Pythium ultimum pathogens, with 45%, 40% and 37.5% inhibition rates, respectively. Chaudhary et al (2020), a total of 31 Trichoderma spp. were studied for their antagonistic potentials against Rhizoctonia solani in dual culture, effect of volatile and nonvolatile metabolites on the growth inhibition of test pathogen under in-vitro condition.…”

Morphological and Molecular Characterization of Trichoderma spp. from Rhizosphere Soil and Their Antagonistic Activity against Fusarium spp.

“…Lysis of hyphae of Fusarium with close contact of T. harzianum hyphae was observed. Isolates of T. harzianum were found effective against various phytopathogens like R. solani [13], Sclerotinia sclerotiorum [40] Fusarium oxysporum [8] etc. in dual culture assay.…”

“…Continuous production of exo-chitinases by Trichoderma spp. damages cell-walls of the pathogen, thereby discharging oligomers, which play a crucial part in the inhibition of phytopathogens [13]. Thus, the isolation and identification of such a valuable antagonistic fungus would help in commercialization and ultimately developing a superior strategy to eliminate pathogens that minimize the crop losses.…”