Biocontrol arsenals of bacterial endophyte: An imminent triumph against clubroot disease

Ahmed, Ayesha; Munir, Shahzad; Li, Yongmei; He, Ping; Wu, Yixin; He, Yueqiu

doi:10.1016/j.micres.2020.126565

Cited by 51 publications

(26 citation statements)

References 136 publications

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“…However, biocontrol of plant diseases can be the most promising alternative to continuously failing treatments and endophyte-mediated biocontrol offers consistent results due to intimate association with its host and shared niche with the pathogen where endophyte could fully manifest their antagonistic potential, whereas the non-endophytic biocontrol agents often fail under field conditions due to fierce competition in the rhizosphere and harsh environmental conditions (Ahmed et al, 2020;Blacutt et al, 2020;Trivedi et al, 2020). In biocontrol programs, native endophytes could be used to maintain long-term colonization inside the citrus host and eliminate CLas through niche and nutrient competition (Munir et al, 2018b).…”

Section: Introductionmentioning

confidence: 99%

Defeating Huanglongbing Pathogen Candidatus Liberibacter asiaticus With Indigenous Citrus Endophyte Bacillus subtilis L1-21

Munir

et al. 2022

Front. Plant Sci.

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Huanglongbing (HLB) has turned into a devastating botanical pandemic of citrus crops, caused by Candidatus Liberibacter asiaticus (CLas). However, until now the disease has remained incurable with very limited control strategies available. Restoration of the affected microbiomes in the diseased host through the introduction of an indigenous endophyte Bacillus subtilis L1-21 isolated from healthy citrus may provide an innovative approach for disease management. A novel half-leaf method was developed in vitro to test the efficacy of the endophyte L1-21 against CLas. Application of B. subtilis L1-21 at 104 colony forming unit (cfu ml−1) resulted in a 1,000-fold reduction in the CLas copies per gram of leaf midrib (107 to 104) in 4 days. In HLB-affected citrus orchards over a period of 2 years, the CLas incidence was reduced to < 3%, and CLas copies declined from 109 to 104 g−1 of diseased leaf midribs in the endophyte L1-21 treated trees. Reduction in disease incidence may corroborate a direct or an indirect biocontrol effect of the endophytes as red fluorescent protein-labeled B. subtilis L1-21 colonized and shared niche (phloem) with CLas. This is the first large-scale study for establishing a sustainable HLB control strategy through citrus endophytic microbiome restructuring using an indigenous endophyte.

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Section: Introductionmentioning

confidence: 99%

Defeating Huanglongbing Pathogen Candidatus Liberibacter asiaticus With Indigenous Citrus Endophyte Bacillus subtilis L1-21

Munir

et al. 2022

Front. Plant Sci.

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“…Over the past few decades, important disease-management methods in the form of resistant cultivars and chemical control have been adopted, but the results were not fruitful [ 3 , 11 ]. Therefore, biological control through potent endophytes and rhizobacteria is considered a practical approach for treating and preventing clubroot disease [ 11 , 32 ]. The application of a single biocontrol agent (BCAs) cannot efficiently control clubroot disease due to the highly virulent nature of P. brassicae [ 1 ].…”

Section: Discussionmentioning

confidence: 99%

Microbial Consortia: An Engineering Tool to Suppress Clubroot of Chinese Cabbage by Changing the Rhizosphere Bacterial Community Composition

Zhang

Ahmed

Dai

et al. 2022

Biology

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Clubroot disease, caused by Plasmodiophora brassicae, is a serious threat to Chinese cabbage (Brassica rapa subsp. pekinensis) production, which results in extensive yield losses. At present, clubroot control mainly depends upon pesticides, which provoke food-safety concerns, and the application of sole biocontrol agents cannot successfully control the disease. In this study, we investigated the effect of Bacillus cereus BT-23, Lysobacter antibioticus 13-6, and Lysobacter capsici ZST1-2 as sole strains, intra-/inter-genus co-culture, and microbial consortia on clubroot disease, plant growth, and rhizosphere bacterial diversity in a field experiment. The microbial consortia efficiently controlled the incidence of clubroot disease, with a biocontrol effect of about 65.78%, by decreasing the soil acidity and enhancing the yield (17,662.49 kg/acre). The high-throughput sequencing results demonstrated that the phyla Proteobacteria and Bacteroidetes were present in high relative abundance in the rhizosphere soil of the Chinese cabbage. Furthermore, Firmicutes was found as a unique phylum in the rhizosphere soil of CK-H and T1-T7, except for CK-D. The application of microbial consortia recovers the imbalance in indigenous microbial communities. Therefore, we conclude that microbial consortia can reduce the clubroot incidence in Chinese cabbage by decreasing the soil acidity and altering the diversity and structure of rhizosphere bacterial communities. This study highlights the potential of microbial consortia as an engineering tool to control devastating soilborne diseases in commercial crops.

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“…In addition to free-living microorganisms in the rhizosphere or epiphytic-living microorganisms, endophytic-living microorganisms can also contribute to biological control. Ahmed et al [ 85 ] provide an overview of research carried out with endophytic bacteria and fungi as biocontrol agents. Mostly, endophytic bacteria derived from the rhizosphere enter the plant and colonize the plant tissue without any negative effect on the plant [ 86 ].…”

Section: Clubroot Control Using Beneficial Microorganismsmentioning

confidence: 99%

Control Strategies of Clubroot Disease Caused by Plasmodiophora brassicae

Struck

Rüsch

Strehlow³

2022

Microorganisms

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The clubroot disease caused by the soil-borne pathogen Plasmodiophora brassicae is one of the most important diseases of cruciferous crops worldwide. As with many plant pathogens, the spread is closely related to the cultivation of suitable host plants. In addition, temperature and water availability are crucial determinants for the occurrence and reproduction of clubroot disease. Current global changes are contributing to the widespread incidence of clubroot disease. On the one hand, global trade and high prices are leading to an increase in the cultivation of the host plant rapeseed worldwide. On the other hand, climate change is improving the living conditions of the pathogen P. brassicae in temperate climates and leading to its increased occurrence. Well-known ways to control efficiently this disease include arable farming strategies: growing host plants in wide crop rotations, liming the contaminated soils, and using resistant host plants. Since chemical control of the clubroot disease is not possible or not ecologically compatible, more and more alternative control options are being investigated. In this review, we address the challenges for its control, with a focus on biological control options.

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Biocontrol arsenals of bacterial endophyte: An imminent triumph against clubroot disease

Cited by 51 publications

References 136 publications

Defeating Huanglongbing Pathogen Candidatus Liberibacter asiaticus With Indigenous Citrus Endophyte Bacillus subtilis L1-21

Defeating Huanglongbing Pathogen Candidatus Liberibacter asiaticus With Indigenous Citrus Endophyte Bacillus subtilis L1-21

Microbial Consortia: An Engineering Tool to Suppress Clubroot of Chinese Cabbage by Changing the Rhizosphere Bacterial Community Composition

Control Strategies of Clubroot Disease Caused by Plasmodiophora brassicae

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