Biocontrol Effect of <i>Bacillus subtilis</i> YPS-32 on Potato Common Scab and Its Complete Genome Sequence Analysis

Zhou, Yingjun; Li, Qing; Peng, Zheng; Zhang, Juan; Li, Jianghua

doi:10.1021/acs.jafc.2c00274

Cited by 17 publications

(12 citation statements)

References 39 publications

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“…Pesticides are indispensable in agriculture and play a critical role in fulfilling rising demands for food productivity and quality. − Unfortunately, environmental pollution caused by the widespread use of agrochemicals cannot be ignored. , Biological control has become a more acceptable technique to control plant diseases or pests and overcome environmental challenges. − Pseudomonas species can produce a wide range of novel bioactive metabolites and play an important role in biological control. − …”

Section: Introductionmentioning

confidence: 99%

Production of Antibacterial Questiomycin A in Metabolically Engineered Pseudomonas chlororaphis HT66

Guo

Wang

et al. 2022

J. Agric. Food Chem.

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Pseudomonas chlororaphis has been demonstrated as a valuable source of antimicrobial metabolites for plant disease biocontrol and biopesticide development. Although phenazine-1-carboxylic acid (PCA) secreted by P. chlororaphis has been commercialized as an antifungal biopesticide, it shows poor antibacterial activity. Questiomycin A, with versatile antibacterial activities, is mainly discovered in some well-known phenazine-producing strains but not in Pseudomonas. Its low titer hinders practical applications. In this work, a metabolite was first identified as Questiomycin A in P. chlororaphis-derived strain HT66ΔphzBΔNat. Subsequently, Questiomycin A has been elucidated to share the same biosynthesis process with PCA by gene deletion and in vitro assays. Through rational metabolic engineering, heterologous phenoxazinone synthase introduction, and medium optimization, the titer reached 589.78 mg/L in P. chlororaphis, the highest production reported to date. This work contributes to a better understanding of Questiomycin A biosynthesis and demonstrates a promising approach to developing a new antibacterial biopesticide in Pseudomonas.

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Section: Introductionmentioning

confidence: 99%

Production of Antibacterial Questiomycin A in Metabolically Engineered Pseudomonas chlororaphis HT66

Guo

Wang

et al. 2022

J. Agric. Food Chem.

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“…However, most of the present studies focus on the beneficial mechanism of probiotics on the host and few research pay attention to the genomic characteristics of probiotics. Whole genome sequence analysis can more comprehensively evaluate the safety of Bacillus subtilis and gradually deepen from traditional phenotypic analysis to molecular mechanism and genetic characteristics ( 43 , 44 ). To further understand of the genomic bioinformatics characteristics of Bacillus subtilis isolated from yaks, this study conducted the whole genome analysis to analyze the genome characteristics and functional predictions based on the previous research on the in vitro probiotic characteristics of Bacillus subtilis .…”

Section: Discussionmentioning

confidence: 99%

Complete genome analysis of Bacillus subtilis derived from yaks and its probiotic characteristics

Wang

Lin

et al. 2023

Front. Vet. Sci.

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Probiotics have attracted attention due to their multiple health benefits to the host. Yaks inhabiting the Tibetan plateau exhibit excellent disease resistance and tolerance, which may be associated with their inner probiotics. Currently, research on probiotics mainly focuses on their positive effects on the host, but information regarding their genome remains unclear. To reveal the potential functional genes of Bacillus subtilis isolated from yaks, we sequenced its whole genome. Results indicated that the genomic length of Bacillus subtilis was 866,044,638 bp, with 4,429 coding genes. The genome of this bacteria was composed of one chromosome and one plasmid with lengths of 4,214,774 and 54,527 bp, respectively. Moreover, Bacillus subtilis contained 86 tRNAs, 27 rRNAs (9 16S_rRNA, 9 23S_rRNA, and 9 5S_rRNA), and 114 other ncRNA. KEGG annotation indicated that most genes in Bacillus subtilis were associated with biosynthesis of amino acids, carbon metabolism, purine metabolism, pyrimidine metabolism, and ABC transporters. GO annotation demonstrated that most genes in Bacillus subtilis were related to nucleic acid binding transcription factor activity, transporter activity, antioxidant activity, and biological adhesion. EggNOG uncovered that most genes in Bacillus subtilis were related to energy production and conversion, amino acid transport and metabolism, carbohydrate transport and metabolism. CAZy annotation found glycoside hydrolases (33.65%), glycosyl transferases (22.11%), polysaccharide lyases (3.84%), carbohydrate esterases (14.42%), auxiliary activities (3.36%), and carbohydrate-binding modules (22.59%). In conclusion, this study investigated the genome and genetic properties of Bacillus subtilis derived from yaks, which contributed to understanding the potential prebiotic mechanism of probiotics from the genetic perspective.

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“…Potato common scab is one of the main diseases of potato that is di cult to control [14,15] .We previously showed that the biocontrol strain B. subtilis YPS-32 can antagonize the pathogen S. scabies, with good application results in the eld tests; however, the speci c biocontrol mechanisms are still unclear [13] . An antagonistic interaction exists between a biocontrol strain and a pathogen; when biocontrol strains are infected by a pathogen, the expression of speci c genes and proteins is up-regulated in an attempt to resist infection.…”

Section: Disscucionmentioning

confidence: 99%

“…Several studies have shown that Bacillus can produce a series of antibacterial substances, such as lipopeptides, polyketides, siderophores, and bacteriocins, which are effectively used in the biological control of plant pathogens [16,17] . For example, fengycin played a good role in the biological control of potato scab [5,13] . In this study, ve proteins involved in the biosynthetic pathway of fengycin were signi cantly up-regulated in B. subtilis YPS-32 after 24 h co-culturing with S. scabies.…”

Section: Disscucionmentioning

confidence: 99%

“…We previously showed that B. subtilis YPS-32 successfully antagonizes the potato scab pathogen S. scabies; however, the biocontrol mechanisms of its inhibitory effect are unclear [13] . This study are mainly focused on the changes in genes (proteins) involved in the synthesis of metabolites of B. subtilis YPS-32 after S. scabies infection using transcriptomic and proteomic sequencing, respectively, in an effort to to study the biocontrol mechanisms of B. subtilis YPS-32 metabolites.…”

Section: Introductionmentioning

confidence: 99%

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Uncovering the biocontrol mechanism of Bacillus subtilis YPS-32 antagonism of Streptomyces scabies infection through transcriptome and proteome analysis

Zhou

Peng

et al. 2023

Preprint

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Background The bacterium Streptomyces scabies is the main pathogen causing potato common scab. Efforts to control S. scabies infection using the bacterial strain Bacillus subtilis YPS-32 as a biocontrol agent have been excellent; however, the underlying biocontrol mechanism is not clear. Results In this study, we first determined the key time points of B. subtilis YPS-32 inhibition of S. scabiesgrowth by analyzing the interaction of the two bacterial species in co-culture. We then analyzed differential changes in gene and protein expression patterns in B. subtilis YPS-32 after 0 h (control) and 24 h (peak inhibition) co-culture, using transcriptomic and proteomic sequencing, respectively. We found that the expression levels of genes and proteins related to the synthesis of the antimicrobial metabolites fengycin, bacillaene, and bacilysin were significantly up-regulated in B. subtilis YPS-32 after 24 h co-culture. In addition, the protein, WP_003245811.1 related to bacillibactinexport was also upregulated in co-culture. Conclusions The fengycin, bacillaene, bacilysin and bacillibactin were potential antibacterial metabolites of B. subtilis YPS-32.This research will provide support for the further molecular mechanism research of B. subtilis YPS-32 metabolites.

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Biocontrol Effect of Bacillus subtilis YPS-32 on Potato Common Scab and Its Complete Genome Sequence Analysis

Cited by 17 publications

References 39 publications

Production of Antibacterial Questiomycin A in Metabolically Engineered Pseudomonas chlororaphis HT66

Production of Antibacterial Questiomycin A in Metabolically Engineered Pseudomonas chlororaphis HT66

Complete genome analysis of Bacillus subtilis derived from yaks and its probiotic characteristics

Uncovering the biocontrol mechanism of Bacillus subtilis YPS-32 antagonism of Streptomyces scabies infection through transcriptome and proteome analysis

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