Biocontrol efficiency and characterization of insecticidal protein from sugarcane endophytic Serratia marcescens (SM) against oriental armyworm Mythimna separata (Walker)

Lin, Dong-Jiang; Zhou, Jiang-xiong; Ali, Ahmad; Fu, Hua-ying; Gao, San-ji; Jin, Lin; Fang, Yong; Wang, Jin-da

doi:10.1016/j.ijbiomac.2024.129978

International Journal of Biological Macromolecules

2024

DOI: 10.1016/j.ijbiomac.2024.129978

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Biocontrol efficiency and characterization of insecticidal protein from sugarcane endophytic Serratia marcescens (SM) against oriental armyworm Mythimna separata (Walker)

Dong-Jiang Lin,

Jiang-xiong Zhou,

Ahmad Ali

et al.

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Cited by 3 publications

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Effects of two transglutaminases on innate immune responses in the oriental armyworm, Mythimna separata

Zhu,

Furukawa

2024

Insect Science

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Transglutaminase (TGase) is a key enzyme that mediates hemolymph coagulation and is thought to contribute to the elimination of pathogenic microorganisms in invertebrates. The objective of this study was to elucidate the involvement of TGase in insect immune responses via functional analysis of this enzyme in the oriental armyworm, Mythimna separata, using recombinant proteins and RNA interference technique. We identified two TGase genes, mystgase1 and mystgase2, in Mythimna separata and found that both genes are expressed in all surveyed tissues in M. separata larvae. Significant changes were induced in hemocytes following Escherichia coli injection. Injection of Gram‐positive bacteria (Micrococcus luteus) and Gram‐negative bacteria (Escherichia coli and Serratia marcescens) into larvae triggered a time‐specific induction of both mystgase1 and mystgase2 in hemocytes. Recombinant MysTGase1 and MysTGase2 proteins bound to both E. coli and M. luteus, localizing within bacterial clusters and resulting in agglutination in a Ca2+‐dependent manner. The hemocytes of larvae injected with recombinant MysTGase1 or MysTGase2 exhibited enhanced phagocytic ability against E. coli, improved in vivo bacterial clearance, and increased resistance to S. marcescens, decreasing larval mortality rate. Conversely, RNA interference targeting mystgase1 or mystgase2 significantly reduced hemocyte phagocytic capability, decreased bacterial clearance, and increased susceptibility to S. marcescens infection, thereby increasing larval mortality rate. The findings of this study are anticipated to expand our understanding of the function of TGases within insect immune responses and may contribute to developing new pest control strategies.

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Effects of two transglutaminases on innate immune responses in the oriental armyworm, Mythimna separata

Zhu,

Furukawa

2024

Insect Science

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The efficacy of Serratia nematodiphila and Neem Azal T/S on Macrosiphum rosae: new approaches in biological control

Dilmen,

Şanver,

Kaplan

et al. 2024

International Journal of Agriculture Environment and Food Sciences

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The rose aphid, Macrosiphum rosae (L.) (Hemiptera: Aphididae), is one of the most common pests of rose plants. This study evaluated the effects of four different doses of Neem Azal-T/S, containing the active ingredient Azadirachtin A, and a dose of Serratia nematodiphila (1x108 cfu/ml) on M. rosae over 72 hours. The experiment was conducted in a climate chamber under controlled conditions (25±1 °C, 60±5% relative humidity, and a 16:8 light-dark photoperiod). The results showed that Neem Azal-T/S led to mortality rates of 12.5%, 17.5%, 60%, and 77.5%, respectively, while S. nematodiphila resulted in a 78% mortality rate after 72 hours. In the control group, mortality was 0.75%, while mortality rates for the treatment groups were 1.25 (Neem_1), 1.75 (Neem_2), 6.00 (Neem_3), 7.00 (Neem_4), and 8.25 (S. nematodiphila). Statistical analyses showed significant differences between all treatment groups and the control. In conclusion, this study demonstrated that both Neem Azal-T/S and S. nematodiphila significantly increased mortality rates in M. rosae compared with the control. Additionally, this study is the first record of the presence of S. nematodiphila in Türkiye and the first information on its entomopathogenic effect on M. rosae in the worldwide, supporting the potential of biological methods in managing rose aphids and emphasizing the importance of biological control strategies in agricultural pest management.

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An overview of symbiotic and pathogenic interactions at the fungi-plant interface under environmental constraints

Mishra,

Srivastava,

Singh

et al. 2024

Front. Fungal Biol.

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The complex and dynamic interactions between fungi and plants constitute a critical arena in ecological science. In this comprehensive review paper, we explore the multifaceted relationships at the fungi-plant interface, encompassing both mutualistic and antagonistic interactions, and the environmental factors influencing these associations. Mutualistic associations, notably mycorrhizal relationships, play a pivotal role in enhancing plant health and ecological balance. On the contrary, fungal diseases pose a significant threat to plant health, agriculture, and natural ecosystems, such as rusts, smuts, powdery mildews, downy mildews, and wilts, which can cause extensive damage and lead to substantial economic losses. Environmental constraints encompassing abiotic and biotic factors are elucidated to understand their role in shaping the fungi-plant interface. Temperature, moisture, and soil conditions, along with the presence of other microbes, herbivores, and competing plants, significantly influence the outcome of these interactions. The interplay between mutualism and antagonism is emphasised as a key determinant of ecosystem health and stability. The implications of these interactions extend to overall ecosystem productivity, agriculture, and conservation efforts. The potential applications of this knowledge in bioremediation, biotechnology, and biocontrol strategies emphasise the importance of adapting to climate change. However, challenges and future directions in this field include the impacts of climate change, emerging fungal pathogens, genomic insights, and the role of the fungi-plant interface in restoration ecology. Hence, this review paper provides a comprehensive overview of fungi-plant interactions, their environmental influences, and their applications in agriculture, conservation, and ecological restoration.

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Biocontrol efficiency and characterization of insecticidal protein from sugarcane endophytic Serratia marcescens (SM) against oriental armyworm Mythimna separata (Walker)

Cited by 3 publications

References 36 publications

Effects of two transglutaminases on innate immune responses in the oriental armyworm, Mythimna separata

Effects of two transglutaminases on innate immune responses in the oriental armyworm, Mythimna separata

The efficacy of Serratia nematodiphila and Neem Azal T/S on Macrosiphum rosae: new approaches in biological control

An overview of symbiotic and pathogenic interactions at the fungi-plant interface under environmental constraints

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