Biocontrol of Bacillus cereus for Anopheles sp larvae

Abstract

Biological control is one of the best ways to eradicate mosquitoes. The eradication can be done by using natural antagonists such as microbes. The research objectives are to determine the concentration value of local isolate Bacillus cereus which cause 50% mortality and 95% mortality toward Anopheles larvae (instars 3) and to know how long Bacillus cereus AOT9 can survive in the LC95 until mortality of larvae Anopheles decreases to 70% in laboratory condition. Research was experimentally done with six different concentrations (10, 20, 30, 40, 50, and 60 ppm) using three replications. The result showed that there is no mortality of Anopheles larvae in the control sample, 43 % mortality for each repetition in 10 ppm, and the highest mortality was 98 % in 68 ppm. Also, the mortality of Anopheles larvae was 50 % in the concentration of 20 ppm to 30 ppm. Probit analysis for Bacillus cereus at LC50 was 27.93 ppm and at LC95 was 57.43 ppm. The residual test for mortality of Anopheles larvae in the first 48 hours was 96.7 % of the total in three replications and then decrease to 70 % in 336 hours or day 14.