Biocontrol of bacterial wilt disease in tomato using Bacillus subtilis strain R31

Abstract

Bacterial wilt disease caused by Ralstonia solanacearum is a widespread, severe plant disease. Tomato (Solanum lycopersicum), one of the most important vegetable crops worldwide, is particularly susceptible to this disease. Biological control offers numerous advantages, making it a highly favorable approach for managing bacterial wilt. In this study, the results demonstrate that treatment with the biological control strain Bacillus subtilis R31 significantly reduced the incidence of tomato bacterial wilt. In addition, R31 directly inhibits the growth of R. solanacearum, and lipopeptides play an important role in this effect. The results also show that R31 can stably colonize the rhizosphere soil and root tissues of tomato plants for a long time, reduce the R. solanacearum population in the rhizosphere soil, and alter the microbial community that interacts with R. solanacearum. This study provides an important theoretical basis for elucidating the mechanism of B. subtilis as a biological control agent against bacterial wilt and lays the foundation for the optimization and promotion of other agents such as R31.

Antibacterial Activity and Mechanism of Three Root Exudates from Mulberry Seedlings against Ralstonia pseudosolanacearum

“…The antibacterial activities of ginger extract against the ginger bacterial wilt pathogen R. solanacearum [ 28 ]. It has been shown that maintaining a lower concentration of root exudates can stimulate an increase in ginseng rhizome biomass and enhance the accumulation of ginsenosides within the rhizomes [ 48 ]. The Type III Secretion System (T3SS) of R. solanacearum is one of its most important virulence factors [ 49 ].…”

“…In this research, we employed the Rp strain MRS-5 (GDMCC1.3250), initially isolated in our lab from diseased mulberry plants located in Luogang, Guangdong Province. The cultivation process involved growing MRS-5 on a casamino acid–peptone–glucose (CPG) agar plate [ 53 ], with incubation at 30 °C for 72 h [ 48 ]. Subsequently, a single colony was selected and inoculated into 150 mL of CPG liquid medium.…”

Synthesis and evaluation of pyridine-3-carboxamide analogs as effective agents against bacterial wilt in tomatoes

“…Bacterial wilt incited by R. solanacearum infection is one of the major factor for diseases in tomato and other solanaceous plants that caused drastic loss of tomato fruit worldwide 2 – 5 . The use of traditional pesticides to treat bacterial wilt in plants do not show positive effects and has been proved not very effective 6 , Therefore, discover new bioactive molecules and pesticides agent still represents a daunting task in pesticide science 7 , 8 . About 450 plant species from 54 botanical families have been reported as hosts of R. solanacearum 9 , 10 .…”