Biocontrol of Fusarium wilt of cucumber with Trichoderma longibrachiatum NGJ167 (Rifai)

Kareem, T. A.; Ugoji, O; Aboaba, O

doi:10.9734/bmrj/2016/28208

Cited by 11 publications

(13 citation statements)

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“…This study recruited sixteen new Trichoderma antagonistic strains assigned, on the base of the variations of the rRNA ITS and translation elongation factor 1-α gene partial sequences, to three different species, T. longibrachiatum, T. atroviride , and T. harzianum . Several stains of these species are well-known as BCAs of many pathogens affecting vegetables, including our targets (Bastakoti et al, 2017 ): they are proposed alone, being part of complex microbial consortia or activating suppressive organic amendments (Kareem et al, 2016 ; Wang et al, 2019 ; Chilosi et al, 2020 ). The macroscopic and microscopic examination of the selected strains showed interesting characters such as the profuse sporulation, the ability to secrete secondary metabolites in the medium changing its pigmentation and the capability of some to produce a volatile compound with the typical coconut-like aroma.…”

Section: Discussionmentioning

confidence: 99%

Functional Hyperspectral Imaging by High-Related Vegetation Indices to Track the Wide-Spectrum Trichoderma Biocontrol Activity Against Soil-Borne Diseases of Baby-Leaf Vegetables

et al. 2021

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Research has been increasingly focusing on the selection of novel and effective biological control agents (BCAs) against soil-borne plant pathogens. The large-scale application of BCAs requires fast and robust screening methods for the evaluation of the efficacy of high numbers of candidates. In this context, the digital technologies can be applied not only for early disease detection but also for rapid performance analyses of BCAs. The present study investigates the ability of different Trichoderma spp. to contain the development of main baby-leaf vegetable pathogens and applies functional plant imaging to select the best performing antagonists against multiple pathosystems. Specifically, sixteen different Trichoderma spp. strains were characterized both in vivo and in vitro for their ability to contain R. solani, S. sclerotiorum and S. rolfsii development. All Trichoderma spp. showed, in vitro significant radial growth inhibition of the target phytopathogens. Furthermore, biocontrol trials were performed on wild rocket, green and red baby lettuces infected, respectively, with R. solani, S. sclerotiorum and S. rolfsii. The plant status was monitored by using hyperspectral imaging. Two strains, Tl35 and Ta56, belonging to T. longibrachiatum and T. atroviride species, significantly reduced disease incidence and severity (DI and DSI) in the three pathosystems. Vegetation indices, calculated on the hyperspectral data extracted from the images of plant-Trichoderma-pathogen interaction, proved to be suitable to refer about the plant health status. Four of them (OSAVI, SAVI, TSAVI and TVI) were found informative for all the pathosystems analyzed, resulting closely correlated to DSI according to significant changes in the spectral signatures among health, infected and bio-protected plants. Findings clearly indicate the possibility to promote sustainable disease management of crops by applying digital plant imaging as large-scale screening method of BCAs' effectiveness and precision biological control support.

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Section: Discussionmentioning

confidence: 99%

Functional Hyperspectral Imaging by High-Related Vegetation Indices to Track the Wide-Spectrum Trichoderma Biocontrol Activity Against Soil-Borne Diseases of Baby-Leaf Vegetables

et al. 2021

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“…Supplementary Materials: The following are available online at http://www.mdpi.com/2076-2607/8/2/236/s1: Figure S1: Analysis of culture-independent endophytic actinobacteria communities at genus level top 30 in the cucumber roots; Figure S2: Strain HAAG3-15 showed antagonism to F. oxysporum f. sp. Cucumerinum; Figure S3: 1 H NMR (600 MHz) spectrum of compound 1 in MeOD; Figure S4: 13 C NMR (150 MHz) spectrum of compound 1 in MeOD; Figure S5: ESI-MS spectrum of compound 1.…”

Section: Discussionmentioning

confidence: 99%

“…The symptoms of the disease are vascular and root wilt which eventually cause plant death [11,12]. Chemical control agents are implicated in ecological, environmental, and human health problems, and pathogens can develop resistance to them [13]. Traditional ways of crop rotation and seeding grafting could be applicable for controlling Fusarium wilt; however, these methods are high-cost and laborious [14].…”

Section: Introductionmentioning

confidence: 99%

“…Up to now, agricultural scientists paid much attention to an efficient, environmentally friendly, and sustainable method, biocontrol, which is used for protecting plants against soil-borne diseases [15]. A number of antagonistic microbes were investigated and studied to control various plant pathogens, such as Pseudomonas spp., Bacillus spp., Streptomyces spp., Trichoderma spp., and Paenibacillus spp [13,[16][17][18][19][20][21][22]. Kareem et al reported that Trichoderma longibrachiatum NGJ167 could be used to control Fusarium wilt of cucumber [13], Bacillus subtilis 9407 was recorded as a biocontrol agent against bacterial fruit blotch of melon [20], and Pseudomonas aeruginosa BRp3 could reportedly be applied in controlling bacterial leaf blight of rice [22].…”

Section: Introductionmentioning

confidence: 99%

“…A number of antagonistic microbes were investigated and studied to control various plant pathogens, such as Pseudomonas spp., Bacillus spp., Streptomyces spp., Trichoderma spp., and Paenibacillus spp [13,[16][17][18][19][20][21][22]. Kareem et al reported that Trichoderma longibrachiatum NGJ167 could be used to control Fusarium wilt of cucumber [13], Bacillus subtilis 9407 was recorded as a biocontrol agent against bacterial fruit blotch of melon [20], and Pseudomonas aeruginosa BRp3 could reportedly be applied in controlling bacterial leaf blight of rice [22]. Streptomyces genus is one of the most efficient groups, with the capacity of preventing plant fungal diseases [17,19,21,23,24].…”

Section: Introductionmentioning

confidence: 99%

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Community Structures and Antifungal Activity of Root-Associated Endophytic Actinobacteria in Healthy and Diseased Cucumber Plants and Streptomyces sp. HAAG3-15 as a Promising Biocontrol Agent

Wang

et al. 2020

Microorganisms

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Microorganisms related to plant roots are vital for plant growth and health and considered to be the second genome of the plant. When the plant is attacked by plant pathogens, the diversity and community structure of plant-associated microbes might be changed. The goal of this study is to characterize differences in root-associated endophytic actinobacterial community composition and antifungal activity between Fusarium wilt diseased and healthy cucumber and screen actinobacteria for potential biological control of Fusarium wilt of cucumber. In the present research, three healthy plants (also termed “islands”) and three obviously diseased plants (naturally infected by F. oxysporum f. sp. cucumerinum) nearby the islands collected from the cucumber continuous cropping greenhouse were chosen as samples. Results of culture-independent and culture-dependent analysis demonstrated that actinomycetes in the healthy roots were significantly more abundant than those of diseased roots. Moreover, there were seven strains with antifungal activity against F. oxysporum f. sp. cucumerinum in healthy cucumber roots, but only one strain in diseased cucumber roots. Out of these eight strains, the isolate HAAG3-15 was found to be best as it had the strongest antifungal activity against F. oxysporum f. sp. cucumerinum, and also exhibited broad-spectrum antifungal activity. Thus, strain HAAG3-15 was selected for studying its biocontrol efficacy under greenhouse conditions. The results suggested that the disease incidence and disease severity indices of cucumber Fusarium wilt greatly decreased (p < 0.05) while the height and shoot fresh weight of cucumber significantly increased (p < 0.05) after inoculating strain HAAG3-15. On the basis of morphological characteristics, physiological and biochemical properties and 100% 16S ribosomal RNA (rRNA) gene sequence similarity with Streptomyces sporoclivatus NBRC 100767T, the isolate was assigned to the genus Streptomyces. Moreover, azalomycin B was isolated and identified as the bioactive compound of strain HAAG3-15 based on analysis of spectra using a bioactivity-guided method. The stronger antifungal activity against F. oxysporum f. sp. cucumerinum, the obvious effect on disease prevention and growth promotion on cucumber seedlings in the greenhouse assay, and the excellent broad-spectrum antifungal activities suggest that strain HAAG3-15 could be developed as a potential biocontrol agent against F. oxysporum f. sp. cucumerinum used in organic agriculture. These results suggested that the healthy root nearby the infected plant is a good source for isolating biocontrol and plant growth-promoting endophytes.

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Effect of Trichoderma spp. on Fusarium wilt disease of tomato

2019

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Biocontrol of Fusarium wilt of cucumber with Trichoderma longibrachiatum NGJ167 (Rifai)

Cited by 11 publications

References 0 publications

Functional Hyperspectral Imaging by High-Related Vegetation Indices to Track the Wide-Spectrum Trichoderma Biocontrol Activity Against Soil-Borne Diseases of Baby-Leaf Vegetables

Functional Hyperspectral Imaging by High-Related Vegetation Indices to Track the Wide-Spectrum Trichoderma Biocontrol Activity Against Soil-Borne Diseases of Baby-Leaf Vegetables

Community Structures and Antifungal Activity of Root-Associated Endophytic Actinobacteria in Healthy and Diseased Cucumber Plants and Streptomyces sp. HAAG3-15 as a Promising Biocontrol Agent

Effect of Trichoderma spp. on Fusarium wilt disease of tomato

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