Biocontrol of Korean Ginseng Root Rot Caused by Phytophthora cactorum Using Antagonistic Bacterial Strains ISE13 and KJ1R5

Sang, Mee Kyung; Chiang, Mae-Hee; Yi, Eun Seob; Park, Kuen Woo; Kim, Ki Deok

doi:10.5423/ppj.2006.22.1.103

Cited by 9 publications

(8 citation statements)

References 10 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Four bacterial strains were reported to be effective for control of ginseng diseases such as Phytophthora blight caused by Phytophthora cactorum and root rot caused by C. destructans (Bae et al, 2004;Sang et al, 2006). Two bacterial isolates strongly antagonistic to C. destructans were isolated during the survey of biocontrol agents for ginseng root rot.…”

mentioning

confidence: 99%

Biocontrol Efficacies of Bacillus Species Against Cylindrocarpon destructans Causing Ginseng Root Rot

Jang¹,

Kim²,

Kim³

2011

The Plant Pathology Journal

View full text Add to dashboard Cite

Two antifungal bacteria were selected from forest soils during the screening of microorganisms antagonistic to Cylindrocarpon destructans, a cause of ginseng root rot. The antifungal bacteria were identified as Bacillus subtilis (I4) and B. amyloliquefaciens (yD16) based on physiological and cultural characteristics, the Biolog program, and 16S rRNA gene sequencing analyses. Antagonistic activity of both bacterial isolates to C. destructans increased with increasing temperature. More rapid starch hydrolytic activity of the bacteria was seen on starch agar at higher temperatures than at lower temperatures, and in the higher density inoculum treatment than in the lower density inoculum treatment. The bacterial isolates failed to colonize ginseng root the root tissues inoculated with the bacteria alone at an inoculum density of 1 × 10 6 cfu/ml, but succeeded in colonizing the root tissues co-inoculated with the bacteria and C. destructans. Scanning electron microscopy showed that the pathogen was damaged by the low-density inoculum treatment with the bacterial isolates as much as by the high-density inoculum treatment. Both bacterial isolates were more effective in reducing root rot when they were treated at a concentration of 1 × 10 6 cfu/ ml than at 1 × 10 8 cfu/ml. Also, only the former treatment induced prominent wound periderm formation, related to structural defense against pathogen infection. The results suggest that the bacterial antagonists may have high potential as biocontrol agents against ginseng root rot at relatively low-inoculum concentrations.

show abstract

mentioning

confidence: 99%

Biocontrol Efficacies of Bacillus Species Against Cylindrocarpon destructans Causing Ginseng Root Rot

Jang¹,

Kim²,

Kim³

2011

The Plant Pathology Journal

View full text Add to dashboard Cite

show abstract

“…Root rot on ginseng Sang et al 2006Cohen 1981a, Cohen 1981b, Cohen 1982 Pythium species Damping-off, root-rot Van West et al 2003, Schmitthenner 1985, Cohen 1981a damping-off pathogen" to tomato and cucumber seedlings Balghouthi et al 2013 Root rot disease of legumes Cohen 1981b, Cohen 1982, Gutter 1983, de Cock et al 2008, Balghouthi et al 2012 Souli …”

Section: Disease Caused Referencesmentioning

confidence: 99%

“…Colonization of bacteria (e.g. Enterobacter cloacae) resulted in a competitive exclusion of nutrients from Pythium, Phytopthora capsici, and Phytophthora cactorum (Nelson 1988, Sang et al 2007, Sang et al 2006. Various antibiotics and lytic enzymes produced by microorganisms revealed antagonism against oomycetes (Dunne et al 1997, Timmusk et al 2009, Muthukumar et al 2011.…”

Section: Biological Control Of Oomycetes and Mechanisms Involvedmentioning

confidence: 99%

Biological Control of Oomycetous Plant Pathogens: A Review

Shrestha

Park

Shrestha

et al. 2015

Nepal Journal of Science and Technology

View full text Add to dashboard Cite

show abstract

“…C. destructans 18-21 o C pH 7.0 (Rahman Punja, 2005). , , Pythium Phytophthora (Blank Murray, 1998;Cho , 1996;Sang , 2006).…”

Section: Destructansmentioning

confidence: 99%

Environmental Factors on the Development of Root Rot on Ginseng Caused by Cylindrocarpon destructans

Lee¹,

Han²,

Lee³

et al. 2014

Research in Plant Disease

View full text Add to dashboard Cite

The fungus Cylindrocarpon destructans is the cause of root rot in many ginseng production areas in Korea. A total of 57 isolates of C. destructans were recovered from diseased roots in a survey of ginseng-growing fields from 2011-2012. Among these isolates, 37% were classified as highly virulent (causing lesions on unwounded mature roots) and 61% were weakly virulent(causing lesions only on previously wounded roots). Radial growth of highly and weakly virulent isolates on potato dextrose agar was highest at 20 o C and there was no growth at 35 o C. Mycelial mass production was significantly (P = 0.05) lower at pH 7.0 compared with pH 5.0. To study the effects of pH (5.0 and 7.0) and wounding on disease development, ginseng roots were grown hydroponically in nutrient solution. Lesions were significantly larger (P < 0.01) at pH 5.0 compared with pH 7.0 and wounding enhanced disease by a highly virulent isolate at both pHs. In artificially infested soil, 2-yearold ginseng roots were most susceptible to Cylindrocarpon root rot among all root ages tested (1 to 4 years) when evaluated using a combined scale of disease incidence and severity. Root rot severity was significantly (P<0.05) enhanced by increasing the inoculum density from 3.5 × 10 2 cfu/g of soil to 2.0×10 3 cfu/g of soil.

show abstract

Biocontrol of Korean Ginseng Root Rot Caused by Phytophthora cactorum Using Antagonistic Bacterial Strains ISE13 and KJ1R5

Cited by 9 publications

References 10 publications

Biocontrol Efficacies of Bacillus Species Against Cylindrocarpon destructans Causing Ginseng Root Rot

Biocontrol Efficacies of Bacillus Species Against Cylindrocarpon destructans Causing Ginseng Root Rot

Biological Control of Oomycetous Plant Pathogens: A Review

Environmental Factors on the Development of Root Rot on Ginseng Caused by Cylindrocarpon destructans

Contact Info

Product

Resources

About