Biocontrol of postharvest decay using a new strain of Pseudomonas syringae CPA-5 in different cultivars of pome fruits

Nunes, Carla; Usall, J.; Teixidó, Neus; Abadías, Maribel; Asensio, A.; Vinas, I.

doi:10.2137/145960607781635877

Cited by 9 publications

(4 citation statements)

References 30 publications

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“…Biological control, using microorganisms antagonistic to the fungal plant pathogens, has gained considerable attention and appears to be promising as a viable supplement or alternative to chemical control (Spadaro and Gullino 2004;Nunes et al 2009). A whole range of living organisms has been proposed as potential biocontrol agents, on pome fruits (Viñas et al 1998;Nunes et al 2001;Torres et al 2005;Mounir et al 2007;Nunes et al 2007), stone fruit (Pusey and Wilson 1984;Bonaterra et al 2003), grapes, cherries (Kurtzman and Droby 2001;Schena et al 2003) and citrus fruits (Chalutz and Wilson 1990;Smilanick and Denis-Arrue 1992). However, the success and widespread use of biofungicides, remains limited.…”

Section: Introductionmentioning

confidence: 99%

Production of the biocontrol agent Pantoea agglomerans PBC-1 in a stirred tank reactor by batch and fed-batch cultures

Manso

Nunes

Raposo

et al. 2009

World J Microbiol Biotechnol

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Concerns about food safety as well as the development of resistance to many fungicides by major postharvest pathogens have increased recently. Biological control, using microorganisms antagonistic to the fungal plant pathogens, appears to be promising as an alternative to fungicides. The microbial biocontrol agent has to be produced on an industrial scale, maintaining its biocontrol efficacy. The purpose of the current study was to optimize the conditions for microbial biomass production of the biocontrol agent Pantoea agglomerans PBC-1 in a 2-l mechanically stirred reactor (STR), defining mixing and mass transfer technological parameters and the growth kinetics for different saccharides. In the batch mode, different impellers and spargers were tested. Despite the oxygen mass transfer improvement achieved with marine propeller combined with porous sparger, the biomass did not increase, if compared with the use of a Rushton turbine and L-sparger, pointing out the relevance of a radial flux for better broth homogenization. Different carbon sources were used: sucrose, glucose and fructose; each of which led to viable populations 3.9 9 10 9 , 1.4 9 10 9 , 3.9 9 10 9 c.f.u/ml, respectively, after 20 h of incubation. Fedbatch technology allows the maintenance of high cell viability for longer periods of time in the stationary growth phase, which can be crucial for the scale-up of biocontrol agent production process that is achieved together with a reduction of 85% on the incidence caused by the pathogens, brought about by fresh microbial biomass preparation on artificially wounded apples or oranges, stored for 7 days at 25°C against Penicillium expansum and Penicillium digitatum.

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Section: Introductionmentioning

confidence: 99%

Production of the biocontrol agent Pantoea agglomerans PBC-1 in a stirred tank reactor by batch and fed-batch cultures

Manso

Nunes

Raposo

et al. 2009

World J Microbiol Biotechnol

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“…Based on different morphologies and color, colonies were selected and further isolated. Eighteen postharvest fungal antagonists: CPA-1 (Candida sake, Viñas et al, 1998), CPA-2 (Pantoea agglomerans, Nunes et al, 2001), CPA-3 (Pantoea ananatis, Torres et al, 2005), CPA-5 (Pseudomonas syringae, Nunes et al, 2007), CPA-7 (Pseudomonas graminis, Alegre et al, 2013b) and EL 8, PN5, PN6, 128-M, C-9P-21, F-13, F-10, PO, C-9 17, P-12, C-5 10, C-10 5, and RG4 strains (unpublished results); belonging to the Pathology Laboratory collection (IRTA, Lleida), was also tested.…”

Section: Isolation Of Putative Antagonistsmentioning

confidence: 99%

Biopreservative methods to control the growth of foodborne pathogens on fresh-cut lettuce

Oliveira

Abadías

Colás-Medà

et al. 2015

International Journal of Food Microbiology

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“…Therefore, the rhizosphere will be the scenario here considered to review biocontrol Pseudomonas spp. Nevertheless, specific pseudomonads strains have also shown to be effective in the biocontrol of postharvest and fruit trees disease (see, for instance, Nunes et al 2007;Bonaterra et al 2012) although they will not be treated in this chapter.…”

Section: Beneficial Pseudomonas Sppmentioning

confidence: 99%

Pseudomonas Strains that Exert Biocontrol of Plant Pathogens

Mercado‐Blanco

2014

Pseudomonas

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An increasing number of strains of Pseudomonas spp. are being studied for the known benefits they provide to plants. Many of them are natural inhabitants of plant roots and the soil area influenced by them: the rhizosphere. Some are even capable to colonize the root interior and, occasionally, to spread to above-ground organs establishing themselves as beneficial endophytes. The artificial introduction of these strains into target agro-ecosystems can lead to enhanced growth and improved fitness of host plants. These positive effects are usually a consequence of the capacity of bacteria to suppress the attacks by phytopathogenic microorganisms, a phenomenon known as biological control activity. In this chapter, the main modes of action that biocontrol Pseudomonas strains can deploy against plant pathogens are reviewed: antibiosis, competition for nutrients and niches, induction of defense responses, etc. How these bacteria are able to colonize plant and pathogen structures, as prerequisite for effective disease suppression, will be also discussed. A concise section devoted to the complex regulatory networks controlling biological control traits is included as well. Finally, the potential that biocontrol pseudomonads pose as marketable products is briefly presented. The underlying idea is that a much better understanding of the complex, multitrophic interactions taking place among the plant, the pathogen(s), the biocontrol pseudomonads and the environment is instrumental for commercial exploitation, aiming to overcome the frequently-observed problem of biocontrol performance inconsistency. The powerful '-omics' and microscopy approaches currently available are providing fundamental information to acquire such knowledge. The use of biocontrol agents as a complementary measure within integrated disease management strategies should have a promising future in modern sustainable agriculture.

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Biocontrol of postharvest decay using a new strain of Pseudomonas syringae CPA-5 in different cultivars of pome fruits

Cited by 9 publications

References 30 publications

Production of the biocontrol agent Pantoea agglomerans PBC-1 in a stirred tank reactor by batch and fed-batch cultures

Production of the biocontrol agent Pantoea agglomerans PBC-1 in a stirred tank reactor by batch and fed-batch cultures

Biopreservative methods to control the growth of foodborne pathogens on fresh-cut lettuce

Pseudomonas Strains that Exert Biocontrol of Plant Pathogens

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