Biocontrol of root rot in Geranium with antimycotic rhizobateria

Reyad, Nour El-Houda A.; Elsayed, Tarek; Naguib, Deyala M.; Azoz, Samah N.

doi:10.1016/j.rhisph.2022.100607

Cited by 6 publications

(10 citation statements)

References 50 publications

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“…The present study has shown that B. velezensis B63 treatment had a growth-promoting effects on geranium plants and fungicidal effects on plant pathogenic fungi. This outcome corroborates results from a previous study (Reyad et al, 2022), which showed that B. velezensis B63 had antifungal and plant growth-promoting properties. Chitosan similarly exerted positive effects, although less pronounced than those from B63.…”

Section: Discussionsupporting

confidence: 92%

“…The strain B63 was found to produce secondary metabolites related to biological control, including bacilysin, macrolactin, bacillaene, bacillibactin, surfactin, and fengycin, (Helal et al, 2022). Surfactin is important for motility, signalling, and biofilm formation as well as surface colonization (Hafeez et al, 2019;Helal et al, 2022;Reyad et al, 2022).…”

Section: Discussionmentioning

confidence: 99%

“…Bacillus velezensis B63, known for its antibacterial and antifungal activities (Elsayed et al, 2020;Reyad et al, 2022), was obtained from Dr Tarek R. Elsayed, Microbiology Department, Faculty of Agriculture, Cairo University, Giza, Egypt. The strain was cultivated in an Erlenmeyer flask containing 50 mL of Luria-Bertani broth (10 g of tryptone, 5 g of yeast extract, 10 g of NaCl, and 1 liter of distilled water.…”

Section: Bacillus Velezensis (B63) and Culture Conditionsmentioning

confidence: 99%

“…Rhizoctonia solani and M. phaseolina isolated from rotted geranium roots were obtained from our previous work (Reyad et al, 2022).…”

Section: Bacterial In Vitro Antifungal Activity Against Rhizoctonia S...mentioning

confidence: 99%

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Bacillus velezensis B63 and chitosan control root rot, improve growth and alter the rhizosphere microbiome of geranium

ELSAYED,

EL-SAID,

SAFHI

et al. 2024

Phytopathol. Mediterr.

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The root rot complex of geranium plants caused by Rhizoctonia solani and Macrophomina phaseolina is a major threat, and control of these pathogens predominantly relies on chemicals. This study explored multifaceted applications of Bacillus velezensis (strain B63) and chitosan, assessing their biocontrol efficacy against root rot, and their subsequent effects on rhizosphere communities. Strain B63 was antagonistic to R. solani and M. phaseolina. Under field conditions, greatest efficacy was obtained with strain B63 (36% and 33% disease reductions in, respectively, two growing seasons), chitosan soaking + foliar spray 0.2% (CSF 0.2%) (33 and 27% reductions), and 0.1% chitosan soaking + foliar spray (CSF 0.1%) (33 and 26% reductions). These treatments also changed rhizosphere microbiota, as shown by numbers of colony-forming units (CFU) and 16S rRNA gene microbiome analyses. Concomitant with rhizosphere shifts, essential oil yields and composition were positively affected, as shown by gas chromatography analyses. Chitosan soaking + foliar spray 0.2% increased concentrations of citronellol (1.36-fold), geraniol (1.37-fold), citronellyl formate (1.54-fold), and geranyl formate (1.94-fold) in geranium essential oil, compared with the experimental controls. Strain B63 also increased these essential oils by 1.04- to 1.27-fold. B63 also enhanced eugenol levels by 1.35-fold. Treatments with B63 were more effective than chitosan in improving the geranium plant morphological parameters (plant height, numbers of branches, biomass). These results show that B. velezensis strain B63 treatments have potential for enhancing yields and product quality from geranium plant under root rot infection.

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Section: Discussionsupporting

confidence: 92%

Section: Discussionmentioning

confidence: 99%

Section: Bacillus Velezensis (B63) and Culture Conditionsmentioning

confidence: 99%

“…Rhizoctonia solani and M. phaseolina isolated from rotted geranium roots were obtained from our previous work (Reyad et al, 2022).…”

Section: Bacterial In Vitro Antifungal Activity Against Rhizoctonia S...mentioning

confidence: 99%

See 2 more Smart Citations

Bacillus velezensis B63 and chitosan control root rot, improve growth and alter the rhizosphere microbiome of geranium

ELSAYED,

EL-SAID,

SAFHI

et al. 2024

Phytopathol. Mediterr.

Self Cite

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“…Clay pots with a diameter of 30 cm that had been formalin-disinfected and were filled with peat moss, clay, and washed sand (1:1:1 v/v) were infected with 3% (w/w) of each pathogen separately. In the week before transplantation, the infected soil was irrigated two to three times (Reyad et al, 2022).…”

Section: Fungal Pathogens Culturingmentioning

confidence: 99%

Antifungal Activity of Thyme and Clove Essential Oil Nanoemulsions against Pothos Root Rot

Attia,

Mohamad,

Baiumy

et al. 2024

Egyptian Journal of Agricultural Sciences

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Fungi, including Rhizoctonia solani and Sclerotium rolfsii, pose a significant threat to agricultural productivity, causing substantial crop yield and quality losses. The excessive use of conventional fungicides raises concerns regarding their environmental impact and human health. This study explores the antifungal potential of thyme and clove oil nanoemulsions as a sustainable alternative. Nanoemulsions were prepared using high-energy ultrasonication and exhibited remarkable stability, with homogenous particle size distributions and low polydispersity index values. In vitro assays demonstrated complete fungal growth inhibition at 3000 ppm for both oils. Pot bioassays revealed significant antifungal activity, with thyme oil nanoemulsion reducing R. solani and S. rolfsii root rot by 75% and 50%, respectively. Clove oil nanoemulsion also effectively suppressed these pathogens by 41.67% and 25%, respectively. Thyme and clove oil nanoemulsions treatments significantly stimulated root growth under fungal challenge. In this regard, plants treated with thyme oil nanoemulsion showed remarkable increases in root length (4.61-fold and 11.18-fold), fresh weight (28.59-fold and 45.62-fold), and dry weight (48.29 and 48.43-fold) when exposed to R. solani and S. rolfsii infection, respectively, compared to untreated control. Clove oil nanoemulsion also significantly enhanced these root attributes, with remarkable increases in length (1.87-fold for R. solani and 4.12-fold for S. rolfsii), fresh weight (11.09fold for R. solani and 21.31-fold for S. rolfsii), and dry weight (25.14-fold for R. solani and 16-fold for S. rolfsii). Molecular modeling studies supported the observed antifungal efficacy. In conclusion, thyme and clove essential oil nanoemulsions are promising candidates that act as natural fungicides for managing root rot diseases.

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Antimicrobial activity of geranium (Pelargonium graveolens) essential oil and its encapsulation in carioca bean starch ultrafine fibers by electrospinning

Santos,

Fonseca,

Jansen-Alves

et al. 2024

International Journal of Biological Macromolecules

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Biocontrol of root rot in Geranium with antimycotic rhizobateria

Cited by 6 publications

References 50 publications

Bacillus velezensis B63 and chitosan control root rot, improve growth and alter the rhizosphere microbiome of geranium

Bacillus velezensis B63 and chitosan control root rot, improve growth and alter the rhizosphere microbiome of geranium

Antifungal Activity of Thyme and Clove Essential Oil Nanoemulsions against Pothos Root Rot

Antimicrobial activity of geranium (Pelargonium graveolens) essential oil and its encapsulation in carioca bean starch ultrafine fibers by electrospinning

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