Biocontrol of strawberry fruit infected by Botrytis cinerea: Effects on the microbial communities on fruit assessed by next‐generation sequencing

Abstract

Fruit grey mould, caused by the fungus Botrytis cinerea, is known to be a harmful disease of strawberry at postharvest stage. However, effects of an application of biological control agents (BCAs) on strawberry fruit in terms of shift in the microbial community are still unknown. The present research aimed to investigate the effects of an application of BCAs on postharvest microbial populations present on strawberry fruits. Strawberry plants were sprayed with three kinds of BCA, RhizoVital 42 fl. (Bacillus amyloliquefaciens FZB42), Trianum‐P (Trichoderma harzianum T22) and Naturalis (Beauveria bassiana ATCC 74040), targeting Botrytis cinerea fungus. Control plots were composed of water and fungicide treatments. Microbial communities (bacteria and fungi) were analysed via next‐generation sequencing on an Illumina MiSeq. Analysis of 16S RNA and ITS rRNA sequences indicated that the BCAs application modified both bacterial and fungal community compositions and diversity. An application of two BCAs together had more effects on microbial community composition than a single application. These results suggest that BCAs can modify bacterial and fungal community composition and diversity on strawberry fruits, which may consequently improve the efficiency and establishment of these products on control of postharvest diseases of fruits, such as grey mould.

Soil inoculation of Trichoderma asperellum M45a regulates rhizosphere microbes and triggers watermelon resistance to Fusarium wilt

“…The M45a treatment had a negative effect on the soil fungal community, which was most closely associated with changes in soil nutrients and enzyme activities. For example, Trichoderma , Pseudomonas , Sphingomonas , and Rhodanobacter were positively correlated with ACP, CL, CAT, and SC activities, which may indicate that soil enzyme activities are closely associated with living organisms (Govarthanan et al 2018 ; Cruz et al 2018 ). For species and functional analysis, metagenomic and transcriptomic techniques are required.…”

Soil inoculation of Trichoderma asperellum M45a regulates rhizosphere microbes and triggers watermelon resistance to Fusarium wilt

“…The M45a treatment had a negative effect on the soil fungal community, and the soil fungal community was most closely associated with changes in soil nutrients and enzyme activities. For example, Trichoderma, Pseudomonas, Sphingomonas and Rhodanobacter were positively correlated with ACP, CL, CAT and SC activities, which may indicate that soil enzyme activities are closely associated with living organisms (Govarthanan et al, 2018;Cruz et al, 2018). For species and functional analysis, metagenomic and transcriptomic techniques are required in a future study.…”

Biocontrol Ability of the Bacillus amyloliquefaciens Group, B. amyloliquefaciens, B. velezensis, B. nakamurai, and B. siamensis, for the Management of Fungal Postharvest Diseases: A Review

“…Serenade ASO/Rhapsody (Bayer) consists of B. amyloliquefaciens QST713 and is recommended for the control of anthracnose, gray mold, and fruit rot in postharvest fruit and vegetables . RhizoVital 42 (ABiTEP) and Taegro (Syngenta) contain B. amyloliquefaciens FZB42, while DoubleNickel55 (Certis) contains B. amyloliquefaciens D747. ,, B. amyloliquefaciens 83 has been commercialized as Fungifree AB, and a B. velezensis strain has been commercialized as Botrybel (Agricaldes) . According to the commercial instructions, RhizoVital 42, Taegro, and DoubleNickel55 can be used for the control of various pathogens, such as Botryti s spp., in postharvest fruit, cucurbits, tubers, and vegetables.…”