Biocontrol of Sugarcane Smut Disease by Interference of Fungal Sexual Mating and Hyphal Growth Using a Bacterial Isolate

Liu, Shiyin; Lin, Nuoqiao; Chen, Yumei; Liang, Zhibin; Liao, Lisheng; Lv, Minna; Chen, Yufan; Tang, Yingxin; He, Fei; Chen, Shaohua; Zhou, Jianuan; Zhang, Lian-Hui

doi:10.3389/fmicb.2017.00778

Cited by 26 publications

(22 citation statements)

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“…campestris Xc1 Pathogen of crucifers black rot [ 69 ] 9.68 ± 0.36 0 0 0 Pseudomonas aeruginosa PAO1 Pathogen of cystic fibrosis [ 70 ] 4.04 ± 0.43 0 0 0 Fusarium oxysporum f.sp. cubense FOC4 Pathogen of banana wilt [ 71 ] 6.17 ± 0.33 3.95 ± 0.39 3.32 ± 0.22 0 Rhizoctonia solani AG-1 IA Pathogen of rice sheath blight [ 72 ] 4.07 ± 0.23 1.31 ± 0.09 0.70 ± 0.15 0 Magnaporthe oryzae B157 Pathogen of rice blast [ 73 ] 4.70 ± 0.28 1.98 ± 0.34 1.50 ± 0.31 0 Peronophythora litchi Pathogen of litchi downy blight [ 74 ] 11.69 ± 0.34 8.71 ± 0.15 3.48 ± 0.21 3.72 ± 0.22 Colletotrichum capsici Pathogen of capsicum anthracnose Lab storage 5.45 ± 0.14 5.26 ± 0.13 4.79 ± 0.10 0 C. gloeosporioides Pathogen of mango anthracnose Lab storage 4.91 ± 0.10 4.06 ± 0.20 3.59 ± 0.42 0 Sporisorium scitamineum Pathogen of sugarcane smut [ 75 ] 2.60 ± 0.06 2.27 ± 0.54 1.51 ± 0.22 0 …”

Section: Resultsmentioning

confidence: 99%

Dickeya zeae strains isolated from rice, banana and clivia rot plants show great virulence differentials

Chen

et al. 2018

BMC Microbiol

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BackgroundDickeya zeae is the causal agent of maize and rice foot rot diseases, but recently it was also found to infect banana and cause severe losses in China. Strains from different sources showed significant diversity in nature, implying complicated evolution history and pathogenic mechanisms.ResultsD. zeae strains were isolated from soft rot banana plants and ornamental monocotyledonous Clivia miniata. Compared with D. zeae strain EC1 isolated from rice, clivia isolates did not show any antimicrobial activity, produced less extracellular enzymes, had a much narrow host ranges, but released higher amount of extracellular polysaccharides (EPS). In contrast, the banana isolates in general produced more extracellular enzymes and EPS than strain EC1. Furthermore, we provided evidence that the banana D. zeae isolate MS2 produces a new antibiotic/phytotoxin(s), which differs from the zeamine toxins produced by rice pathogen D. zeae strain EC1 genetically and in its antimicrobial potency.ConclusionsThe findings from this study expanded the natural host range of D. zeae and highlighted the genetic and phenotypic divergence of D. zeae strains. Conclusions can be drawn from a series of tests that at least two types of D. zeae strains could cause the soft rot disease of banana, with one producing antimicrobial compound while the other producing none, and the D. zeae clivia strains could only infect monocot hosts. D. zeae strains isolated from different sources have diverse virulence characteristics.Electronic supplementary materialThe online version of this article (10.1186/s12866-018-1300-y) contains supplementary material, which is available to authorized users.

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Section: Resultsmentioning

confidence: 99%

Dickeya zeae strains isolated from rice, banana and clivia rot plants show great virulence differentials

Chen

et al. 2018

BMC Microbiol

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“…Pseudomonas sp. ST4 displayed a potent inhibitory activity on the sexual mating of S. scitamineum, and evidence suggests that the active components are small molecules diffusible in PDA agar medium (Liu et al ., ). Thermal stability assay showed that Pseudomonas sp.…”

Section: Resultsmentioning

confidence: 97%

“…ST4 slightly reduced the disease occurrence, with a disease incidence at about 89%. However, similar to the case of sugarcane trials (Liu et al ., ), when Pseudomonas sp. ST4 grew and inoculated with 2% glucose, its biocontrol efficiency was greatly increased with a disease incidence reduced to only about 15% (Table ).…”

Section: Resultsmentioning

confidence: 99%

“…In our previous study, we have isolated a Pseudomonas sp. ST4 from vegetable rhizosphere in Shantou city, Guangdong Province, China, which showed a potent inhibitory activity against the sexual mating of S. scitamineum without killing the fungal haploid cells (Liu et al ., ). To understand the mechanism of biocontrol, we optimized the fermentation and chromatography conditions for isolation of the active compounds that inhibit the fungal sexual mating.…”

Section: Introductionmentioning

confidence: 97%

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Pseudomonas sp. ST4 produces variety of active compounds to interfere fungal sexual mating and hyphal growth

Liu

Lin

et al. 2018

Microbial Biotechnology

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Sexual mating of compatible sporida is essential for Sporisorium scitamineum to form dikaryotic mycelia and then cause infection on sugarcane. Our previous work identified a Pseudomonas sp. ST4 from a soil sample, which showed a promising biocontrol potential by inhibiting the mating of S. scitamineum sporida and hyphal growth. In this study, we set to isolate the active compounds from Pseudomonas sp. ST4 through solid fermentation. High-performance liquid chromatography (HPLC) separation coupling with bioassay showed that Pseudomonas sp. ST4 produced a range of antimicrobial compounds. Two of the major components were purified following acetate extraction, silica gel and HPLC separation. Nuclear magnetic resonance (NMR) and liquid chromatography-mass spectrometry (LC-MS) analysis identified these active compounds are 4-hydroxybenzaldehyde and indole-3-carbaldehyde respectively. Further analysis showed that the former compound only inhibited the hyphal growth of the fungus at a concentration of 3 mM, while the latter interfered the fungal sexual mating at a concentration of 0.6 mM and affected hyphal growth at a concentration of 2 mM. Treatment of corn plants with 3 mM indole-3-carbaldehyde significantly inhibited corn smut infection, with a control rate up to 94%. Further analysis of the structure and activity relationship revealed that indole has a much stronger inhibitory activity against the fungal sexual mating than indole-3-carbaldehyde. The results from this study provide new agents for control and prevention of the sugarcane smut disease, and the active compounds could also be used to probe the molecular mechanisms of fungal sexual mating.

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“…produce alphahydroxy-ketones; and Xylella fastidiosa and Xanthomonas spp. produce a wide variety of diffusible signal factors (DSFs) (Flavier et al, 1997;Chen et al, 2002;Kendall and Sperandio, 2007;Zhang et al, 2007;Mei et al, 2010;Churchill and Chen, 2011;Winans, 2011;Lee and Zhang, 2015;Ryan et al, 2015;Liu et al, 2017;Zhan et al, 2018). DSF is widely conserved in gram-negative bacterial pathogens (Deng et al, 2011;Zhou et al, 2017).…”

Section: Discussionmentioning

confidence: 99%

Acinetobacter lactucae Strain QL-1, a Novel Quorum Quenching Candidate Against Bacterial Pathogen Xanthomonas campestris pv. campestris

et al. 2019

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Quorum sensing (QS) is a cell-cell communication mechanism among bacterial populations that is regulated through gene expression in response to cell density. The pathogenicity of Xanthomonas campestris pv. campestris (Xcc) is modulated by the diffusible signal factor (DSF)-mediated QS system. DSF is widely conserved in a variety of gram-negative bacterial pathogens. In this study, DSF-degrading bacteria and their enzymes were thoroughly explored as a biocontrol agent against Xcc. The results indicated that a novel DSF-degrading bacterium, Acinetobacter lactucae QL-1, effectively attenuated Xcc virulence through quorum quenching. Lab-based experiments indicated that plants inoculated with QL-1 and Xcc had less tissue decay than those inoculated with Xcc alone. Co-inoculation of strains Xcc and QL-1 significantly reduced the incidence and severity of disease in plants. Similarly, the application of crude enzymes of strain QL-1 substantially reduced the disease severity caused by Xcc. The results showed that strain QL-1 and its enzymes possess promising potential, which could be further investigated to better protect plants from DSF-dependent pathogens.

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Biocontrol of Sugarcane Smut Disease by Interference of Fungal Sexual Mating and Hyphal Growth Using a Bacterial Isolate

Cited by 26 publications

References 23 publications

Dickeya zeae strains isolated from rice, banana and clivia rot plants show great virulence differentials

Dickeya zeae strains isolated from rice, banana and clivia rot plants show great virulence differentials

Pseudomonas sp. ST4 produces variety of active compounds to interfere fungal sexual mating and hyphal growth

Acinetobacter lactucae Strain QL-1, a Novel Quorum Quenching Candidate Against Bacterial Pathogen Xanthomonas campestris pv. campestris

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