Biocontrol of tobacco black shank disease (Phytophthora nicotianae) by Bacillus velezensis Ba168

Guo, Dongsheng; Yuan, Cong; Luo, Yunyan; Chen, YaHan; Lu, Mingli; Chen, Guochan; Ren, Guangwei; Cui, Chuanbin; Zhang, Jiatao; An, Derong

doi:10.1016/j.pestbp.2020.01.004

Cited by 51 publications

(40 citation statements)

References 72 publications

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“…Tobacco black shank, caused by P. nicotianae, is destructive to almost all flue-cured tobacco cultivars and is widespread in many tobacco-growing countries. Bacillus strains represent a promising strategy for the management of this soil-borne disease (Han et al, 2016;Zhang et al, 2017a;Srikhong et al, 2018;Guo et al, 2020).…”

Section: Discussionmentioning

confidence: 99%

“…The severity of tobacco black shank continues to increase each year, causing significant economic losses. In the field, tobacco black shank affects the stem base ( Han et al, 2016 ; Guo et al, 2020 ) and the chemical fungicide metalaxyl is generally used for disease control. However, this practice is not conducive to the development of sustainable tobacco agriculture, as it creates an excessive dependence on chemical agents, leaves fungicide residues, and causes environmental pollution ( Han et al, 2016 ; Guo et al, 2020 ).…”

Section: Introductionmentioning

confidence: 99%

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Whole Genome Sequence of Bacillus velezensis Strain GUMT319: A Potential Biocontrol Agent Against Tobacco Black Shank Disease

Ding

et al. 2021

Front. Microbiol.

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Phytophthora nicotianae causes black shank, a serious soil-borne disease, in tobacco. In this study, the Bacillus strain GUMT319 was isolated from the rhizosphere of healthy tobacco plants grown in a field in Guizhou with a high incidence of tobacco black shank. Genome sequencing revealed that GUMT319 contained a single circular chromosome 3,940,023 bp in length, with 4,053 predicted genes and an average GC content of 46.6%. Based on phylogenomic analyses, GUMT319 was designated as Bacillus velezensis. The genome of GUMT319 contained more than 60 genes and 13 gene clusters that have previously been found to be active in antifungal mechanisms, biofilm formation, and chemotaxis motility. Additionally, confocal laser scanning microscopy and scanning electron microscopy showed that GUMT319 formed a spatially organized biofilm in vivo. In addition, lauric acid negatively regulated biofilm formation. This is the first study to report that nicotine in tobacco root exudates was a chemoattractant for biocontrol Bacillus strains. In this study, we identified new interactions between beneficial microorganisms and tobacco roots in the rhizosphere. Moreover, dual culture tests in vitro showed that GUMT319 inhibited the growth of P. nicotianae and also displayed inhibitory effects against eight other plant pathogens, namely, Colletotrichum scovillei, Colletotrichum capsici, Fusarium carminascens, Sclerotinia sclerotiorum, Alternaria alternata, Phomopsis sp., Phyllosticta sorghina, and Exserohilum turcicum. Furthermore, GUMT319 exhibited > 70% control efficiency against tobacco black shank in field experiments conducted in 2018–2020. Thus, GUMT319 was more effective in controlling the incidence of tobacco black shank than other treatments including fungicide application. Overall, these results suggested that GUMT319 (B. velezensis) could be used as a potential biocontrol agent against tobacco black shank.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Whole Genome Sequence of Bacillus velezensis Strain GUMT319: A Potential Biocontrol Agent Against Tobacco Black Shank Disease

Ding

et al. 2021

Front. Microbiol.

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“…Also, in field trials, Bacillus safensis strain Bsa27 and ( B. velezensis starin Bve2 + Bacillus altitudinis strain Bal13) reduced Heterodera glycines cyst population density at 60 DAP. PGPR Bacillus velezensis Ba168 has been reported as promising biological control agent against tobacco black shank disease caused by pathogen Phytophthora nicotianae, which reduced the disease index in field assays 71 . Recently, Karthika et al 72 isolated bacterium Bacillus cereus KTMA4 from tomato rhizosphere which was found to be effective against phytopathogens Fusarium oxysporum and Alternaria saloni and also, increased the plant height, fresh/wet weight in green house experiments.…”

Section: Discussionmentioning

confidence: 99%

Field evaluation of PGP Bacillus sp. strain D5 native to Crocus sativus, in traditional and non traditional areas, and mining of PGP genes from its genome

Magotra

Bhagat

Ambardar

et al. 2021

Sci Rep

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Native Bacillus sp. strain D5 coded as (Bar D5) has been isolated from the saffron corm that showed plant growth promotion (PGP) properties and also inhibits the growth of corm rot causing Fusarium oxysporum R1 (Fox R1) in-vitro. Bar D5 was more efficient PGP bacterium in comparison to earlier reported native bio-formulations by our group. Pot assays and field evaluation of Bar D5 confirmed its in-vivo efficacy for PGP traits and biocontrol activity as well. Pot trials were followed by field trials at traditional (Kishtwar) and non-traditional (R.S Pura) saffron cultivation areas in Jammu and Kashmir. At both places, Bar D5 bio-formulation treatment led to the increase in root number & length, shoot number & length, flower number and number & weight of daughter corms. Additionally, it also decreased the corm rot disease incidence significantly. Priming of corms with bio-formulation resulted in the reduction of pathogenic fungal load by three fold at the depth of corm sowing from ground level. The shelf life/viability of Bar D5 based bio-formulation was found to be 52% (viable spores) for one year at room temperature. Draft genome sequence of Bar D5 revealed the presence of genes necessary for PGP and biocontrol activity. Further, confirmation of gene sequences and annotation was done by amplification, re-sequencing and mapping of PGP and biocontrol genes on draft genome. Bar D5 based bio-formulation can be provided to companies/researchers interested in saffron cultivation or bio-formulation production for commercial exploitation, since saffron is grown as revenue crop across continents. The present study bridges the gap between genomics and its field application.

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“…After 7 days incubation at 20°C, DI was calculated based on the rating scale of 0 – 9. Where, 0 = no symptoms; 1 = less than one third of the total leaflet/tuber with symptoms; 3 = one third to half of leaflet/tuber with symptoms; 5 = one half to two thirds leaflet/tuber with symptoms; 7 = more than two thirds leaflet/tuber with symptoms; 9 = all leaflet/tuber with symptoms ( Han et al, 2016 ; Guo et al, 2020 ). The DIs were calculated by the following formula ( Balint-Kurti et al, 2007 ; Ding et al, 2017 ):…”

Section: Methodsmentioning

confidence: 99%

Bacillus megaterium WL-3 Lipopeptides Collaborate Against Phytophthora infestans to Control Potato Late Blight and Promote Potato Plant Growth

Wang

Zhang

et al. 2020

Front. Microbiol.

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Oomycete Phytophthora infestans [(Mont.) de Bary] is the cause of potato late blight, a plant disease which poses a serious threat to our global food security and is responsible for huge economic losses worldwide. Lipopeptides produced by Bacillus species are known to be potent antibacterial compounds against many plant pathogens. In this study, we show that Bacillus megaterium WL-3 has an antagonistic effect against potato late blight. Electrospray ionization mass spectrometry (ESI-MS) revealed that lipopeptides derived from the WL-3 strain contained three subfamilies, surfactin (C 13 – C 15 ), Iturin A (C 14 – C 16 ), and Fengycin A (C 15 – C 19 ). The Iturin A and Fengycin A lipopeptide families were each confirmed to have anti-oomycete effects against P. infestans mycelium growth as well as obvious controlling effects against potato late blight in greenhouse experiments and field assays. Furthermore, Iturin A and Fengycin A were able to promote plant photosynthetic efficiency, plant growth, and potato yield. Most importantly, the combination of Iturin A and Fengycin A (I + F) was superior to individual lipopeptides in controlling potato late blight and in the promotion of plant growth. The results of this study indicate that B. megaterium WL-3 and its lipopeptides are potential candidates for the control of late blight and the promotion of potato plant growth.

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Biocontrol of tobacco black shank disease (Phytophthora nicotianae) by Bacillus velezensis Ba168

Cited by 51 publications

References 72 publications

Whole Genome Sequence of Bacillus velezensis Strain GUMT319: A Potential Biocontrol Agent Against Tobacco Black Shank Disease

Whole Genome Sequence of Bacillus velezensis Strain GUMT319: A Potential Biocontrol Agent Against Tobacco Black Shank Disease

Field evaluation of PGP Bacillus sp. strain D5 native to Crocus sativus, in traditional and non traditional areas, and mining of PGP genes from its genome

Bacillus megaterium WL-3 Lipopeptides Collaborate Against Phytophthora infestans to Control Potato Late Blight and Promote Potato Plant Growth

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