Biocontrol of volatile organic compounds obtained from Bacillus subtilis CL2 against Aspergillus flavus in peanuts during storage

Ling, Lijun; Jiang, Kunling; Cheng, Wenting; Wang, Yuanyuan; Pang, Mingmei; Luo, Hong; Lu, Lu; Gao, Kun; Tu, Yixin

doi:10.1016/j.biocontrol.2022.105094

Cited by 17 publications

(4 citation statements)

References 48 publications

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“…Additionally, Liu et al [53] reported Bacillus velezensis HC6 could prevent the colonization of A. avus on corn. More recently, Ling et al [54] reported Bacillus subtilis CL2 against A avus in peanuts during storage. Thus, B. velezensis YE-1 could be a promising biological agent for preventing A. avus contamination in corn and peanuts during storage.…”

Section: Discussionmentioning

confidence: 99%

Inhibitory roles of Bacillus velezensis YE-1 isolated from lichens in Baiyin city, Gansu province against Aspergillus flavus

Zou,

Ling,

et al. 2023

Preprint

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Aspergillus flavus has evoked worldwide attention owing to its serious threat to public health, but how to safely, efficiently, and economically reduce A. flavus contamination remains a great challenge. Here, a Bacillus strain capable of inhibiting A. flavus was isolated from lichens. The antifungal assay showed that Bacillus velezensis YE-1 could inhibit the growth of A. flavus. Scanning and transmission electron microscopy revealed that B. velezensis YE-1 exposure severely deformed the spore and mycelial structure and morphology of A. flavus. Furthermore, the biochemical studies revealed a significant alternation in ergosterol content, mitochondrial membrane potential, nuclear DNA, and aflatoxin synthesis gene in A. flavus exposed to B. velezensis YE-1. In addition, the inhibitory activity of isolated B. velezensis YE-1 was mainly present in the cell-free supernatant, confirming that the heat-resistant non-protein substances produced by B. velezensis YE-1 exerted an antagonistic role on A. flavus. Finally, on corn and peanut kernels, B. velezensis YE-1 can also remarkably improve the disease severity by A. flavus. Therefore, our results showed that B. velezensis YE-1 could be a very promising biocontrol agent for A. flavus and subsequent contamination of food/feed commodities.

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Section: Discussionmentioning

confidence: 99%

Inhibitory roles of Bacillus velezensis YE-1 isolated from lichens in Baiyin city, Gansu province against Aspergillus flavus

Zou,

Ling,

et al. 2023

Preprint

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“…Among the 243 compounds, 34 were identified as having beneficial properties. These included 2,3-Butanedione [37], 2-Heptanone [31], and 2,5-dimethyl, Pyrazine, methyl- [38], etc. The results of this study justify future research to determine the antifungal activity of some of the identified VOCs in order to evaluate their potential bioactivity against antibiotic-resistant bacteria.…”

Section: Analysis Of Vocs By Gc-msmentioning

confidence: 99%

Unveiling the Potential of Bacillus safensis Y246 for Enhanced Suppression of Rhizoctonia solani

Zhu,

Xu,

Wang

et al. 2023

JoF

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Rhizoctonia solani is a significant pathogen affecting various crops, including tobacco. In this study, a bacterial strain, namely Y246, was isolated from the soil of healthy plants and exhibited high antifungal activity. Based on morphological identification and DNA sequencing, this bacterial strain was identified as Bacillus safensis. The aim of this investigation was to explore the antifungal potential of strain Y246, to test the antifungal stability of Y246 by adjusting different cultivation conditions, and to utilize gas chromatography–mass spectrometry (GC-MS) to predict the volatile compounds related to antifungal activity in Y246. In vitro assays demonstrated that strain Y246 exhibited a high fungal inhibition rate of 76.3%. The fermentation broth and suspension of strain Y246 inhibited the mycelial growth of R. solani by 66.59% and 63.75%, respectively. Interestingly, treatment with volatile compounds derived from the fermentation broth of strain Y246 resulted in abnormal mycelial growth of R. solani. Scanning electron microscopy analysis revealed bent and deformed mycelium structures with a rough surface. Furthermore, the stability of antifungal activity of the fermentation broth of strain Y246 was assessed. Changes in temperature, pH value, and UV irradiation time had minimal impact on the antifungal activity, indicating the stability of the antifungal activity of strain Y246. A GC-MS analysis of the volatile organic compounds (VOCs) produced by strain Y246 identified a total of 34 compounds with inhibitory effects against different fungi. Notably, the strain demonstrated broad-spectrum activity, exhibiting varying degrees of inhibition against seven pathogens (Alternaria alternata, Phomopsis. sp., Gloeosporium musarum, Dwiroopa punicae, Colletotrichum karstii, Botryosphaeria auasmontanum, and Botrytis cinerea). In our extensive experiments, strain Y246 not only exhibited strong inhibition against R. solani but also demonstrated remarkable inhibitory effects on A. alternata-induced tobacco brown spot and kiwifruit black spot, with impressive inhibition rates of 62.96% and 46.23%, respectively. Overall, these findings highlight the significant antifungal activity of B. safensis Y246 against R. solani. In addition, Y246 has an excellent antifungal stability, with an inhibition rate > 30% under different treatments (temperature, pH, UV). The results showed that the VOCs of strain Y246 had a strong inhibitory effect on the colony growth of R. solani, and the volatile substances produced by strain Y246 had an inhibitory effect on R. solani at rate of 70.19%. Based on these results, we can conclude that Y246 inhibits the normal growth of R. solani. These findings can provide valuable insights for developing sustainable agricultural strategies.

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“…In addition to volatile organic compounds (VOCs), attention should also be directed towards diffusible and soluble compounds, as they cover a vast array of potentially biologically active substances capable of inhibiting the growth of phytopathogens [ 22 , 23 , 24 , 25 ]. Among these compounds are cyclic lipopeptides belonging to the iturin, surfactin, and fengicin families, which are renowned for their potent activity against a broad spectrum of phytopathogens, including bacteria, fungi, oomycetes, and viruses [ 26 , 27 , 28 ].…”

Section: Introductionmentioning

confidence: 99%

Novel Pseudomonas Species Prevent the Growth of the Phytopathogenic Fungus Aspergillus flavus

Rabiço,

Borelli,

Alnoch

et al. 2024

BioTech

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In response to the escalating demand for sustainable agricultural methodologies, the utilization of microbial volatile organic compounds (VOCs) as antagonists against phytopathogens has emerged as a viable eco-friendly alternative. Microbial volatiles exhibit rapid diffusion rates, facilitating prompt chemical interactions. Moreover, microorganisms possess the capacity to emit volatiles constitutively, as well as in response to biological interactions and environmental stimuli. In addition to volatile compounds, these bacteria demonstrate the ability to produce soluble metabolites with antifungal properties, such as APE Vf, pyoverdin, and fragin. In this study, we identified two Pseudomonas strains (BJa3 and MCal1) capable of inhibiting the in vitro mycelial growth of the phytopathogenic fungus Aspergillus flavus, which serves as the causal agent of diseases in sugarcane and maize. Utilizing GC/MS analysis, we detected 47 distinct VOCs which were produced by these bacterial strains. Notably, certain volatile compounds, including 1-heptoxydecane and tridecan-2-one, emerged as primary candidates for inhibiting fungal growth. These compounds belong to essential chemical classes previously documented for their antifungal activity, while others represent novel molecules. Furthermore, examination via confocal microscopy unveiled significant morphological alterations, particularly in the cell wall, of mycelia exposed to VOCs emitted by both Pseudomonas species. These findings underscore the potential of the identified BJa3 and MCal1 Pseudomonas strains as promising agents for fungal biocontrol in agricultural crops.

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Biocontrol of volatile organic compounds obtained from Bacillus subtilis CL2 against Aspergillus flavus in peanuts during storage

Cited by 17 publications

References 48 publications

Inhibitory roles of Bacillus velezensis YE-1 isolated from lichens in Baiyin city, Gansu province against Aspergillus flavus

Inhibitory roles of Bacillus velezensis YE-1 isolated from lichens in Baiyin city, Gansu province against Aspergillus flavus

Unveiling the Potential of Bacillus safensis Y246 for Enhanced Suppression of Rhizoctonia solani

Novel Pseudomonas Species Prevent the Growth of the Phytopathogenic Fungus Aspergillus flavus

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