Biocontrol Potential of Bacillus amyloliquefaciens against Botrytis pelargonii and Alternaria alternata on Capsicum annuum

Kazerooni, Elham Ahmed; Maharachchikumbura, Sajeewa S. N.; Al-Sadi, Abdullah M.; Kang, Sang Mo; Yun, Byung‐Wook; Lee, In Jung

doi:10.3390/jof7060472

Cited by 29 publications

(13 citation statements)

References 129 publications

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“…Also, our data was consistent with previous studies that the fungal and bacterial communities suppress pathogens via multiple mechanisms 46 , such as direct inhibition, secretion of antimicrobial substances 6,47,48 , activation of the systemic immune response 49 , and biofilm formation in the rhizosphere. Taken together, the different roles of bacteria and fungi in the activation of the tomato innate immune systems, nutrient/niches competition, and secretion of fungicide compounds highlighted the irreplaceable functional roles of Bac and Fun SynComs 34,35 .…”

Section: Discussionmentioning

confidence: 99%

“…Fifty-one CAZ-associated pathways were enriched (FDR-adjusted P < 0.05) in CrossK SynComs compared to the CAZassociated pathways in Bac SynComs and Fun SynComs, such as chitinase, xyloglucan hydrolase, chitin-binding protein, pectin methylesterase, mannan-binding, endoglucanase, cellulases, and lipases (Supplementary Data 19). These enzymes may inhibit the growth of FOL, thereby supporting tomato resistance against FOL and enhancing the survival of tomato plant 34,35 .…”

Section: Tomato Immune Responses Triggered By Different Syncomsmentioning

confidence: 99%

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Cross-kingdom synthetic microbiota supports tomato suppression of Fusarium wilt disease

et al. 2022

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The role of rhizosphere microbiota in the resistance of tomato plant against soil-borne Fusarium wilt disease (FWD) remains unclear. Here, we showed that the FWD incidence was significantly negatively correlated with the diversity of both rhizosphere bacterial and fungal communities. Using the microbiological culturomic approach, we selected 205 unique strains to construct different synthetic communities (SynComs), which were inoculated into germ-free tomato seedlings, and their roles in suppressing FWD were monitored using omics approach. Cross-kingdom (fungi and bacteria) SynComs were most effective in suppressing FWD than those of Fungal or Bacterial SynComs alone. This effect was underpinned by a combination of molecular mechanisms related to plant immunity and microbial interactions contributed by the bacterial and fungal communities. This study provides new insight into the dynamics of microbiota in pathogen suppression and host immunity interactions. Also, the formulation and manipulation of SynComs for functional complementation constitute a beneficial strategy in controlling soil-borne disease.

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Section: Discussionmentioning

confidence: 99%

Section: Tomato Immune Responses Triggered By Different Syncomsmentioning

confidence: 99%

Cross-kingdom synthetic microbiota supports tomato suppression of Fusarium wilt disease

et al. 2022

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“…The enhancing capacity of B. amyloliquefaciens for pepper plant growth is in coherence with Shahzad et al [ 85 ] and Rashad et al [ 19 ], who documented that B. amyloliquefaciens RWL-1 and GGA inoculation significantly enhanced the growth traits of tomato and garlic plants under both diseased and non-diseased conditions of F. oxysporum and S. cepivorum . The extensively distinguished mechanisms for plant growth promotion caused by B. amyloliquefaciens are through phytohormones, providing the essential nutrients, N 2 fixation, and phosphate solubilization [ 18 ]. In addition, endophytic bacteria may promote plant growth through biofertilization [ 19 ].…”

Section: Discussionmentioning

confidence: 99%

“…Amaresan et al [ 16 ] found that endophytic bacteria that were isolated from Capsicum annuum have an antagonistic effect against several phytopathogens. Also, infection by fungal pathogens results in stronger reductions in plant biomass and survival compared to uninfected plants [ 12 , 18 , 19 ]. Bacillus was mentioned in numerous research papers due to its prevalence in many plants, its antibacterial properties [ 20 , 21 , 22 ], and its ability to produce endospores that are UV, pH, temperature, and salinity resistant [ 9 ].…”

Section: Introductionmentioning

confidence: 99%

Evaluation of the Antifungal Activity of Bacillus amyloliquefaciens and B. velezensis and Characterization of the Bioactive Secondary Metabolites Produced against Plant Pathogenic Fungi

Soliman

Khaleil

Metwally

2022

Biology

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Endophytic bacteria are plant-beneficial bacteria with a broad host range. They provide numerous benefits to their hosts, helping them tolerate several biotic and abiotic stresses. An interest has recently been developed in endophytic bacteria which are producing bioactive compounds that contribute to the biological control of various phytopathogens. This research paper aimed to investigate the potentiality of new local strains of endophytic bacteria such as Bacillus amyloliquefaciens and B. velezensis and the production of several antimicrobial metabolites associated with the biocontrol of Alternaria sp., which cause serious diseases and affect important vegetable crops in Egypt. Twenty-five endophytic bacteria isolates were obtained from different plants cultivated in El-Sharkia Governorate, Egypt. Dual culture technique was used to evaluate the bacterial isolates’ antagonistic potentiality against Alternaria sp. and Helminthosporium sp. The most active bacterial isolates obtained were selected for further screening. The antifungal activity of the most active endophytic bacterial isolate was assessed in vivo on pepper seedlings as a biocontrol agent against Alternaria sp. A significant antifungal activity was recorded with isolates C1 and T5 against Alternaria sp. and Helminthosporium sp. The bacterial endophyte discs of C1 and T5 showed the highest inhibitory effect against Alternaria sp. at 4.7 and 3.1 cm, respectively, and Helminthosporium sp. at 3.9 and 4.0 cm, respectively. The most active endophytic isolates C1 and T5 were identified and the 16S rRNA sequence was submitted to the NCBI GenBank database with accession numbers: MZ945930 and MZ945929 for Bacillus amyloliquefaciens and Bacillus velezensis, respectively. The deformity of pathogenic fungal mycelia of Alternaria sp. and Helminthosporium sp. was studied under the biotic stress of bacteria. The culture filtrates of B. amyloliquefaciens and B. velezensis were extracted with different solvents, and the results indicated that hexane was the most efficient. Gas Chromatography-Mass Spectrometry revealed that Bis (2-ethylhexyl) phthalate, Bis (2-ethylhexyl) ester, and N,N-Dimethyldodecylamine were major constituents of the endophytic crude extracts obtained from B. amyloliquefaciens and B. velezensis. The in vivo results showed that Alternaria sp. infection caused the highest disease incidence, leading to a high reduction in plant height and in the fresh and dry weights of pepper plants. With B. amyloliquefaciens application, DI significantly diminished compared to Alternaria sp. infected pepper plants, resulting in an increase in their morphological parameters. Our findings allow for a reduction of chemical pesticide use and the control of some important plant diseases.

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“…are the most used BCAs and appear to be effective in a wide range of pathosystems [92]. Recently, Ramírez-Cariño et al [93] and Kazerooni et al [94] reported that tomato early blight and pepper leaf spot, caused by Alternaria alternata, can be controlled by Bacillus spp. protecting plants from pathogen attack through induction of systemic resistance in the host.…”

Section: Bcas Against Air-borne Pathogensmentioning

confidence: 99%

Advances and Perspectives in the Use of Biocontrol Agents against Fungal Plant Diseases

et al. 2022

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The use of synthetic fungicides to control fungal diseases has growing limitations due to eco-toxicological risks. Therefore, it is necessary to replace or integrate high risk chemicals with safer tools for human health and environment. Consequently, research on the selection, evaluation, characterization, and use of biocontrol agents (BCAs) has consistently increased in the last decades. BCA formulates, particularly in some countries, are still scarce in coping with the growing demand for their use in sustainable agricultural management. To foster development and utilization of new effective bioformulates, there is a need to optimize BCA activity, to share knowledge on their formulation processes and to simplify the registration procedures. Studies based on new molecular tools can significantly contribute to achieve such objectives. The present review provides the state of the art on biocontrol of fungal plant diseases with special emphasis on (i) features of the most studied BCAs; (ii) key strategies to optimize selection and use of BCAs (iii); mechanisms of action of the main BCAs; (iv) molecular tools and metagenomic studies in the selection and use of BCAs; (v) main issues and constraints in the registration and commercialization of BCAs, and (vi) perspectives in the biocontrol of fungal plant diseases.

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Biocontrol Potential of Bacillus amyloliquefaciens against Botrytis pelargonii and Alternaria alternata on Capsicum annuum

Cited by 29 publications

References 129 publications

Cross-kingdom synthetic microbiota supports tomato suppression of Fusarium wilt disease

Cross-kingdom synthetic microbiota supports tomato suppression of Fusarium wilt disease

Evaluation of the Antifungal Activity of Bacillus amyloliquefaciens and B. velezensis and Characterization of the Bioactive Secondary Metabolites Produced against Plant Pathogenic Fungi

Advances and Perspectives in the Use of Biocontrol Agents against Fungal Plant Diseases

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