Biocontrol potential of Bacillus subtilis IBFCBF-4 against Fusarium wilt of watermelon

Zhu, Jiuxiang; Tan, Taimeng; Shen, Airong; Yang, Xiaojuan; Yu, Yongting; Gao, Chunsheng; Li, Zhimin; Cheng, Yi; Chen, Jia; Guo, Long; Sun, Xiangping; Zhang, Yan; Li, Jilie; Zeng, Liangbin

doi:10.1007/s42161-019-00457-6

Cited by 38 publications

(31 citation statements)

References 34 publications

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“…Among these unique characteristics include antibacterial potentials. According to [ 48 ], these potentials are as a result of metabolites they produced. Cell free supernatant (CFS) of bacteria contain these metabolites, and has been recently used by researchers to elicit antimicrobial effects on pathogens.…”

Section: Discussionmentioning

confidence: 99%

In vitro antibacterial activities and molecular characterization of bacterial species isolated from farmlands against selected pathogens

Onajobi

Idowu

Adeyemi

et al. 2020

Biotechnology Reports

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Highlights Screening bacterial isolates for antagonistic potential. Molecular methods for identification of isolates. Six potent antibacterial isolates against the test pathogenic species. Pseudomonas was susceptible to antagonizing strains. Necessity for new effective antibacterial formulation.

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Section: Discussionmentioning

confidence: 99%

In vitro antibacterial activities and molecular characterization of bacterial species isolated from farmlands against selected pathogens

Onajobi

Idowu

Adeyemi

et al. 2020

Biotechnology Reports

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“…Fusarium oxysporum f. sp. niveum (Fon) is a pathogen of destructive soil-borne disease that can cause Fusarium wilt in watermelon and seriously threaten the production of watermelon [ 1 ]. The control of Fon depends on fungicides, such as carbendazim, myclobutanil and diniconazole [ 2 ].…”

Section: Introductionmentioning

confidence: 99%

Comprehensive transcriptomic and proteomic analyses identify intracellular targets for myriocin to induce Fusarium oxysporum f. sp. niveum cell death

Wang

et al. 2021

Microb Cell Fact

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Background Myriocin is a natural product with antifungal activity and is derived from Bacillus amyloliquefaciens LZN01. Our previous work demonstrated that myriocin can inhibit the growth of Fusarium oxysporum f. sp. niveum (Fon) by inducing membrane damage. In this study, the antifungal actions of myriocin against Fon were investigated with a focus on the effects of myriocin on intracellular molecules. Results Analysis of DNA binding and fluorescence spectra demonstrated that myriocin can interact with dsDNA from Fon cells. The intracellular-targeted mechanism of action was also supported by transcriptomic and proteomic analyses; a total of 2238 common differentially expressed genes (DEGs) were identified. The DEGs were further verified by RT-qPCR. Most of the DEGs were assigned metabolism and genetic information processing functions and were enriched in ribosome biogenesis in eukaryotes pathway. The expression of some genes and proteins in ribosome biogenesis in eukaryotes pathway was affected by myriocin, primarily the genes controlled by the C6 zinc cluster transcription factor family and the NFYA transcription factor. Myriocin influenced the posttranscriptional processing of gene products by triggering the main RI (retained intron) events of novel alternative splicing; myriocin targeted key genes (FOXG_09470) or proteins (RIOK2) in ribosome biogenesis in eukaryotes pathway, resulting in disordered translation. Conclusions In conclusion, myriocin was determined to exhibit activity against Fon by targeting intracellular molecules. The results of our study may help to elucidate the antifungal actions of myriocin against Fon.

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“…However, the use of these compounds is detrimental to the living system, posing environmental safety issues, and causes the emergence of resistant pathogenic strains 4 . Many studies have been performed to develop methods for the biological control of plant pathogens either by application of antagonistic microorganisms or their secondary metabolites as an alternative to conventional pesticides [5][6][7][8] . Microbial volatile compounds (MVCs) have been verified to inhibit the growth of pathogens and to improve the health of plants by inducing a systemic resistance 4,6 .…”

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confidence: 99%

Dimethyl disulfide exerts antifungal activity against Sclerotinia minor by damaging its membrane and induces systemic resistance in host plants

Lee

Shukla

Chae

2020

Sci Rep

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Microbial volatile compounds (MVCs) significantly influence the growth of plants and phytopathogens. However, the practical application of MVCs at the field level is limited by the fact that the concentrations at which these compounds antagonize the pathogens are often toxic for the plants. In this study, we investigated the effect of dimethyl disulfide (DMDS), one of the MVCs produced by microorganisms, on the fitness of tomato plants and its fungicidal potential against a fungal phytopathogen, Sclerotinia minor. DMDS showed strong fungicidal and plant growth promoting activities with regard to the inhibition of mycelial growth, sclerotia formation, and germination, and reduction of disease symptoms in tomato plants infected with S. minor. DMDS exposure significantly upregulated the expression of genes related to growth and defense against the pathogen in tomato. Especially, the overexpression of PR1 and PR5 suggested the involvement of the salicylic acid pathway in the induction of systemic resistance. Several morphological and ultrastructural changes were observed in the cell membrane of S. minor and the expression of ergosterol biosynthesis gene was significantly downregulated, suggesting that DMDS damaged the membrane, thereby affecting the growth and pathogenicity of the fungus. In conclusion, the tripartite interaction studies among pathogenic fungus, DMDS, and tomato revealed that DMDS played roles in antagonizing pathogen as well as improving the growth and disease resistance of tomato. Our findings provide new insights into the potential of volatile DMDS as an effective tool against sclerotial rot disease.

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Biocontrol potential of Bacillus subtilis IBFCBF-4 against Fusarium wilt of watermelon

Cited by 38 publications

References 34 publications

In vitro antibacterial activities and molecular characterization of bacterial species isolated from farmlands against selected pathogens

In vitro antibacterial activities and molecular characterization of bacterial species isolated from farmlands against selected pathogens

Comprehensive transcriptomic and proteomic analyses identify intracellular targets for myriocin to induce Fusarium oxysporum f. sp. niveum cell death

Dimethyl disulfide exerts antifungal activity against Sclerotinia minor by damaging its membrane and induces systemic resistance in host plants

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