Biocontrol potential of Pseudomonas stutzeri endophyte from Withania somnifera (Ashwagandha) seed extract against pathogenic Fusarium oxysporum and Rhizoctonia solani

Kumar, Ashutosh; Kumar, Sanjay; Chintagunta, Anjani Devi; Agarwal, Dinesh; Pal, Govind; Singh, Ashutosh Kumar; Simal-Gándara, Jesús

doi:10.1080/03235408.2021.1983384

Cited by 24 publications

(16 citation statements)

References 50 publications

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“…Various bacteria, including Pseudomonas stutzeri , generate these enzymes, which can lyse the fungus Fusarium sp. [ 53 ]. Bhutani et al recovered forty-seven (47) endophytic isolates from Cajanus cajan and Vigna radiata plants and among them, nine isolates potentially synthesized extracellular enzymes [ 54 ].…”

Section: Resultsmentioning

confidence: 99%

Stress-Tolerant Endophytic Isolate Priestia aryabhattai BPR-9 Modulates Physio-Biochemical Mechanisms in Wheat (Triticum aestivum L.) for Enhanced Salt Tolerance

Shahid

Zeyad

Syed

et al. 2022

IJERPH

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In efforts to improve plant productivity and enhance defense mechanisms against biotic and abiotic stresses, endophytic bacteria have been used as an alternative to chemical fertilizers and pesticides. In the current study, 25 endophytic microbes recovered from plant organs of Triticum aestivum L. (wheat) were assessed for biotic (phyto-fungal pathogens) and abiotic (salinity, drought, and heavy metal) stress tolerance. Among the recovered isolates, BPR-9 tolerated maximum salinity (18% NaCl), drought (15% PEG-6000), and heavy metals (µg mL−1): Cd (1200), Cr (1000), Cu (1000), Pb (800), and Hg (30). Based on phenotypic and biochemical characteristics, as well as 16S rDNA gene sequencing, endophytic isolate BPR-9 was recognized as Priestia aryabhattai (accession no. OM743254.1). This isolate was revealed as a powerful multi-stress-tolerant crop growth promoter after extensive in-vitro testing for plant growth-promoting attributes, nutrient (phosphate, P; potassium, K; and zinc, Zn) solubilization efficiency, extracellular enzyme (protease, cellulase, amylase, lipase, and pectinase) synthesis, and potential for antagonistic activity against important fungal pathogens viz. Alternaria solani, Rhizoctonia solani, Fusarium oxysporum, and Ustilaginoidea virens. At elevated salt levels, increases were noted in indole-3-acetic acid; siderophores; P, K, and Zn-solubilization; ACC deaminase; and ammonia synthesized by Priestia aryabhattai. Additionally, under in-vitro plant bioassays, wheat seedlings inoculated with P. aryabhattai experienced superior growth compared to non-inoculated seedlings in high salinity (0–15% NaCl) environment. Under NaCl stress, germination rate, plant length, vigor indices, and leaf pigments of wheat seedlings significantly increased following P. aryabhattai inoculation. Furthermore, at 2%-NaCl, B. aryabhattai greatly and significantly (p ≤ 0.05) decreased relative leaf water content, membrane damage, and electrolyte leakage compared with the non-inoculated control. Catalase, superoxide dismutase, and peroxidase activity increased by 29, 32, and 21%, respectively, in wheat seedlings exposed to 2% NaCl and inoculated with the bacteria. The present findings demonstrate that endophytic P. aryabhattai strains might be used in the future as a multi-stress reducer and crop growth promoter in agronomically important crops including cereals.

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Section: Resultsmentioning

confidence: 99%

Stress-Tolerant Endophytic Isolate Priestia aryabhattai BPR-9 Modulates Physio-Biochemical Mechanisms in Wheat (Triticum aestivum L.) for Enhanced Salt Tolerance

Shahid

Zeyad

Syed

et al. 2022

IJERPH

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“…Since the inoculation of crops with the most efficient plant growth-promoting bacteria (PGPB) is one of the relevant strategies used in sustainable agriculture in salt-affected areas ( Kumar et al, 2020 ), searching for new compatible microorganisms appropriately matched to the plant host and environmental conditions is crucial. P. stutzeri is a well-described bacterium and is characterized as a PGPB due to several of its beneficial metabolic properties, e.g., IAA and siderophore synthesis, nitrogen fixation, antifungal activity, and 1-aminocyclopropane-1-carboxylate (ACC) deaminase synthesis ( Patel and Patel, 2014 ; Han et al, 2015 ; Pham et al, 2017 ; Lami et al, 2020 ; Kumar et al, 2021 ). However, there is a lack of research considering the application potential of K. marisflavi .…”

Section: Discussionmentioning

confidence: 99%

Pseudomonas stutzeri and Kushneria marisflavi Alleviate Salinity Stress-Associated Damages in Barley, Lettuce, and Sunflower

et al. 2022

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Soil salinity is one of the most important abiotic factors limiting plant productivity. The aim of this study was to determine the effect of selected halotolerant plant growth-promoting endophytes (PGPEs, Pseudomonas stutzeri ISE12 and Kushneria marisflavi CSE9) on the growth parameters of barley (Hordeum vulgare), lettuce (Lactuca sativa), and sunflower (Helianthus annuus) cultivated under salt stress conditions. A negative effect of two higher tested salinities (150 and 300 mM NaCl) was observed on the growth parameters of all investigated plants, including germination percentage and index (decreasing compared to the non-saline control variant in the ranges 5.3–91.7 and 13.6–90.9%, respectively), number of leaves (2.2–39.2%), fresh weight (24.2–81.6%); however, differences in salt stress tolerance among the investigated crops were observed (H. annuus > H. vulgare > L. sativa). Our data showed that the most crucial traits affected by endophyte inoculation under salt stress were chlorophyll concentration, leaf development, water storage, root development, and biomass accumulation. Thus, the influence of endophytes was species specific. K. marisflavi CSE9 promoted the growth of all tested plant species and could be considered a universal PGPEs for many plant genotypes cultivated under saline conditions (e.g., increasing of fresh weight compared to the non-inoculated control variant of barley, lettuce, and sunflower in the ranges 11.4–246.8, 118.9–201.2, and 16.4–77.7%, respectively). P. stutzeri ISE12 stimulated growth and mitigated salinity stress only in the case of barley. Bioaugmentation of crops with halotolerant bacterial strains can alleviate salt stress and promote plant growth; however, the selection of compatible strains and the verification of universal plant stress indicators are the key factors.

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“…A disc of each pathogenic strain was placed in the center of the petri dish individually for use as control. Fungal pathogens growth inhibition was expressed as a percentage of radial growth inhibition relative to the control 7 days after incubation at 27 °C (Girish and Bhavya, 2018;Lahlali et al, 2020;Marimuthu et al, 2020;Kumar et al, 2021;Mohiddin et al, 2021). Mycelial growth assessment: After 7 days of inoculation, the inhibition zones were measured and used to determine the inhibition rate using the formula below as described by Benouzza et al (2020) The bacterial strain isolated from the rhizosphere of date palm was characterized by colonies small size, rounded and regular shape, smooth aspect and a white color.…”

Section: Bacteria-pathogens Confrontationmentioning

confidence: 99%

In Vitro Study of Biocontrol Potential of Rhizospheric Microorganisms Against Fusarium Oxysporum F.SP. Albedinis

Abouamama

Anis

Ryme

et al. 2022

Pak. J. Phytopathol.

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Biocontrol potential of Pseudomonas stutzeri endophyte from Withania somnifera (Ashwagandha) seed extract against pathogenic Fusarium oxysporum and Rhizoctonia solani

Cited by 24 publications

References 50 publications

Stress-Tolerant Endophytic Isolate Priestia aryabhattai BPR-9 Modulates Physio-Biochemical Mechanisms in Wheat (Triticum aestivum L.) for Enhanced Salt Tolerance

Stress-Tolerant Endophytic Isolate Priestia aryabhattai BPR-9 Modulates Physio-Biochemical Mechanisms in Wheat (Triticum aestivum L.) for Enhanced Salt Tolerance

Pseudomonas stutzeri and Kushneria marisflavi Alleviate Salinity Stress-Associated Damages in Barley, Lettuce, and Sunflower

In Vitro Study of Biocontrol Potential of Rhizospheric Microorganisms Against Fusarium Oxysporum F.SP. Albedinis

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