Biocontrol potential of the endophytic Bacillus amyloliquefaciens YN201732 against tobacco powdery mildew and its growth promotion

Rong, Jun; Munir, Shahzad; Yang, Heng; Wu, Yixin; Wang, Junwei; He, Ping; Cai, Yongzhan; Wang, Ge; He, Yueqiu

doi:10.1016/j.biocontrol.2019.104160

Cited by 65 publications

(29 citation statements)

References 40 publications

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“…Powdery mildew caused by E. cichoracearum seriously affects the yield and quality of tobacco leaves, and once it occurs, it often results in huge economic losses (Glawe, 2008;Meng, 2014). Recent research found that B. amyloliquefaciens YN201732 has a good therapeutic and protective effect on tobacco powdery mildew, and the fermentation broth of the strain has a certain inhibitory effect on spore germination (Jiao et al, 2020), but the specific mechanism is still unclear. In this study, bacillomycin D, a lipopeptide compound that inhibits the activity of pathogenic fungi, was isolated and proved to play a major role in antagonizing the indicator pathogen F. solani.…”

Section: Discussionmentioning

confidence: 99%

“…Bacillus amyloliquefaciens YN201732 is a beneficial endophytic strain isolated from tobacco seeds that can suppress the growth of E. cichoracearum in tobacco and protect the host from pathogen invasion through efficient root colonization (Jiao et al, 2020). In vitro testing showed that strain YN201732 can effectively antagonize a variety of pathogenic fungi, and the supernatant of B. amyloliquefaciens YN201732 completely inhibited the conidial germination of E. cichoracearum, implying that it may secrete antifungal compounds (Jiao et al, 2020). In this study, the antifungal LPs were investigated using both biochemical and genetic approaches.…”

Section: Introductionmentioning

confidence: 99%

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Bacillus amyloliquefaciens YN201732 Produces Lipopeptides With Promising Biocontrol Activity Against Fungal Pathogen Erysiphe cichoracearum

Rong

Cai

Munir

et al. 2021

Front. Cell. Infect. Microbiol.

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Bacillus amyloliquefaciens YN201732 is an endophytic bacteria with high biocontrol efficiency and broad-spectrum antimicrobial activities. In order to clarify the main active ingredients and their antifungal mechanisms against powdery mildew of tobacco, this study is focused on lipopeptide obtained through acid precipitation and organic solvent extraction. HPLC and LCMS-IT-TOF were used to separate and identify antimicrobial lipopeptides. Findings revealed that bacillomycin D plays an important role against surrogate fungal pathogen Fusarium solani. Synthetic pathways of sfp, bacillomycin D, and fengycin were separately disrupted. The sfp gene knockout mutant B. amyloliquefaciens YN201732M1 only showed minor antagonistic activity against F. solani. While Erysiphe cichoracearum spore germination was inhibited and pot experiments displayed a significant decrease in tobacco powdery mildew. The spore inhibition rate of YN201732M1 was only 30.29%, and the pot experiment control effect was less than 37.39%, which was significantly lower than that of the wild type. The inhibitory effect of mutant YN201732M2 (deficient in the production of bacillomycin D) and mutant YN201732M3 (deficient in the production of fengycin) on the spore germination of E. cichoracearum were 50.22% and 53.06%, respectively, suggesting that both fengycin and bacillomycin D had potential effects on spore germination of powdery mildew. Interestingly, in a greenhouse assay, both B. amyloliquefaciens YN201732M2 and YN201732M3 mutants displayed less of a control effect on tobacco powdery mildew than wild type. The results from in vitro, spore germination, and greenhouse-pot studies demonstrated that antimicrobial lipopeptides especially bacillomycin D and fengycin may contribute to the prevention and control of tobacco powdery mildew. In addition, gene mutation related to lipopeptide synthesis can also affect the biofilm formation of strains.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Bacillus amyloliquefaciens YN201732 Produces Lipopeptides With Promising Biocontrol Activity Against Fungal Pathogen Erysiphe cichoracearum

Rong

Cai

Munir

et al. 2021

Front. Cell. Infect. Microbiol.

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“…, F. graminearum, F. solani, Alternaria alternate etc. [11,12]. Beneficial metabolites produced due to ecological interactions between endophyteplant can be harnessed and employed for multifaceted applications in arena such as agriculture, medicine, bioremediation, and biodegradation.…”

Section: Introductionmentioning

confidence: 99%

Biodiversity and antimicrobial potential of bacterial endophytes from halophyte Salicornia brachiata

Singh

Ghadge

Kumar

et al. 2021

Antonie van Leeuwenhoek

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Untapped natural habitats like halophytes, marsh land, and marine environment are suitable arena for chemical ecology between plants and microbes having environmental impact. Endophytes constitute an ecofriendly option for the promotion of plant growth and to serve as sustainable resources of novel bioactive natural products. The present study focusing on biodiversity of bacterial endophytes from Salicornia brachiata, led to isolation of around 350 bacterial endophytes. Phylogenetic analysis of 63 endophytes revealed 13 genera with 29 different species, belonging to 3 major groups: Firmicutes, Proteobacteria and Actinobacteria. 30% isolates belonging to various genera demonstrated broadspectrum antibacterial and antifungal activities against a panel of human, plant, and aquatic infectious agents. An endophytic isolate Bacillus amyloliquefaciens 5NPA-1, exhibited strong in-vitro antibacterial activity against human pathogen S. aureus and phytopathogen X. campestris. Investigation through LC-MS/MS-based molecular networking and bioactivity-guided purification led to the identification of three bioactive compounds belonging to lipopeptide class on the basis of 1 H-and 13 C-NMR and MS analysis. To our knowledge, this is the first report studying bacterial endophytic biodiversity of Salicornia brachiata and isolation of bioactive compounds from its endophyte. Overall, the present study provides insights into the diversity of endophytes associated with the plants from the extreme environment as rich source of metabolites with remarkable agricultural applications and therapeutic properties.

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“…Jiao et al . also found that the endophyte Bacillus amyloliquefaciens YN201732, isolated from tobacco seeds, controlled tobacco powdery mildew 19 . Compared with chemical fungicides, endophytes have many advantages as biological control agents (BCAs), such as being nontoxic, environmentally friendly, safe to use, and cost‐effective 20,21 .…”

Section: Introductionmentioning

confidence: 99%

Endophytic fungus Phomopsis liquidambarisB3 induces rice resistance to RSRD caused by Fusarium proliferatum and promotes plant growth

et al. 2021

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BACKGROUND Rice spikelet rot disease (RSRD) is an emerging disease that significantly reduces rice yield and quality. In this study, we evaluated the potential use of the broad‐spectrum endophytic fungus Phomopsis liquidambaris B3 as a biocontrol agent against RSRD. We also compared the control effects of different treatments, including chemical fungicides and treatment with multiple strains and single strains in combination or individually, against RSRD. The objective of this study was to find an effective and environmentally friendly control strategy to reduce the occurrence of RSRD and improve the rice yield. RESULTS In pot experiments, the effect of B3 alone was better than that of fungicide or combined measures. The results showed that root colonization by B3 significantly reduced the incidence and disease index of RSRD by 41.0% and 53.8%, respectively. This was related to enhanced superoxide dismutase (SOD), peroxidase (POD), and polyphenol oxidase (PPO) activity, and to significantly upregulated expression levels of OsAOX, OsLOX, OsPAL, and OsPR10 in rice. Moreover, B3 improved the diversity of the bacterial community rather than the fungal community in the rice rhizosphere. It also led to a decrease in Fusarium proliferatum colonization and fumonisin content in the grain. Finally, root development was markedly promoted after B3 inoculation, and the yield improved by 48.60%. The result of field experiments showed that the incidence of RSRD and the fumonisin content were observably reduced in rice receiving B3, by 24.41% and 37.87%, respectively. CONCLUSION The endophytic fungus Phomopsis liquidambaris B3 may become an effective tool to relieve rice spikelet rot disease. © 2020 Society of Chemical Industry

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Biocontrol potential of the endophytic Bacillus amyloliquefaciens YN201732 against tobacco powdery mildew and its growth promotion

Cited by 65 publications

References 40 publications

Bacillus amyloliquefaciens YN201732 Produces Lipopeptides With Promising Biocontrol Activity Against Fungal Pathogen Erysiphe cichoracearum

Bacillus amyloliquefaciens YN201732 Produces Lipopeptides With Promising Biocontrol Activity Against Fungal Pathogen Erysiphe cichoracearum

Biodiversity and antimicrobial potential of bacterial endophytes from halophyte Salicornia brachiata

Endophytic fungus Phomopsis liquidambarisB3 induces rice resistance to RSRD caused by Fusarium proliferatum and promotes plant growth

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