Bioconversion of apple peels (Malus domestica) to polyhydroxybutyrate using statistical design to optimize process parameters through Bacillus thuringiensis via solid-state fermentation

Saeed, Shagufta; Firyal, Sehrish; Tayyab, Muhammad; Irfan, Muhammad; Din, Ayesha Mohy ud; Mehmood, Tahir

doi:10.1007/s13399-022-02762-0

Cited by 7 publications

(2 citation statements)

References 25 publications

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“…Since the construction of E. coli strains that can grow with methanol as the sole carbon source is still difficult [ 9 ], in this study, the PHB biosynthesis from methanol was conducted via whole-cell biocatalysis ( Figure 4 ). Although the final PHB content from methanol (6.19%) was not as high as those previously reported (ranging from 20% to 29% PHB content) from sugar-based carbon sources [ 26 ] or other carbon sources [ 41 , 42 , 43 ], a significant increase of 65% in PHB content with the MCC pathway was observed relative to that with only the RuMP pathway through statistical analysis. The experimental results confirm the importance of the combination of NOG with RuMP to form the complete MCC pathway, which can obviously facilitate the biosynthesis of acetyl-CoA–derived chemicals from one-carbon compounds.…”

Section: Discussioncontrasting

confidence: 64%

Engineering Escherichia coli for Poly-β-hydroxybutyrate Production from Methanol

et al. 2023

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The naturally occurring one-carbon assimilation pathways for the production of acetyl-CoA and its derivatives often have low product yields because of carbon loss as CO2. We constructed a methanol assimilation pathway to produce poly-3-hydroxybutyrate (P3HB) using the MCC pathway, which included the ribulose monophosphate (RuMP) pathway for methanol assimilation and non-oxidative glycolysis (NOG) for acetyl-CoA (precursor for PHB synthesis) production. The theoretical product carbon yield of the new pathway is 100%, hence no carbon loss. We constructed this pathway in E. coli JM109 by introducing methanol dehydrogenase (Mdh), a fused Hps–phi (hexulose-6-phosphate synthase and 3-phospho-6-hexuloisomerase), phosphoketolase, and the genes for PHB synthesis. We also knocked out the frmA gene (encoding formaldehyde dehydrogenase) to prevent the dehydrogenation of formaldehyde to formate. Mdh is the primary rate-limiting enzyme in methanol uptake; thus, we compared the activities of three Mdhs in vitro and in vivo and then selected the one from Bacillus methanolicus MGA3 for further study. Experimental results indicate that, in agreement with the computational analysis results, the introduction of the NOG pathway is essential for improving PHB production (65% increase in PHB concentration, up to 6.19% of dry cell weight). We demonstrated that PHB can be produced from methanol via metabolic engineering, which provides the foundation for the future large-scale use of one-carbon compounds for biopolymer production.

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Section: Discussioncontrasting

confidence: 64%

Engineering Escherichia coli for Poly-β-hydroxybutyrate Production from Methanol

et al. 2023

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“…The bacterial isolate’s ideal incubation temperature was used, and 0.5 ml of the inoculums was introduced to 5 ml of sterile basal medium that had been optimally supplemented with various carbon and nitrogen sources 17 .…”

Section: Methodsmentioning

confidence: 99%

Extracellular L-Asparaginase Synthesis Bacillus niacin Isolation, Optimization, and Characterization from Marine Saltern Sediment Sources

Abdul Wahab,

Palaniyandi,

Wyson

et al. 2023

AJMB

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Background: Asparagine is an amino acid that can be converted into aspartic acid and ammonia by the enzyme L-asparaginase. Some forms of cancer, such Acute Lymphoblastic Leukaemia (ALL) and Non-Hodgkin Lymphoma (NHL), respond well to this enzyme when employed as a chemotherapeutic drug. The purpose of this research was to find bacteria that can manufacture the enzymes L-asparaginasein marine slattern sediment which can be employed in commercial and industrial scale production. Methods: All of the strains were identified as Bacillus niacini spp. by biochemical and molecular testing. The strain belongs to the Bacillus genus, according to nutritional, biochemical, PCR and 16srRNA sequencing data. Results: According to the findings of this research, Bacillus niacin spp. have the potential to create a substance that is helpful in a variety of medical applications. The results of this study hint to the possibility that bacteria have the ability to produce antimicrobial compounds, which have the potential to be successful in a wide variety of environments. Conclusion: Numerous opportunities may arise for researchers interested in utilizing the medical potential of enzyme-producing bacteria if they are successfully isolated and screened from aquatic and terrestrial habitats.

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Statistical Design-Driven Optimization of Polyhydroxy-Butyrate (PHB) Production Using Sugarcane Bagasse by Brevibacterium sp. (PP989436) Strain

Preethi Rathna,

Naveen Kishore,

Kulandhaivel

2024

J Polym Environ

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Bioconversion of apple peels (Malus domestica) to polyhydroxybutyrate using statistical design to optimize process parameters through Bacillus thuringiensis via solid-state fermentation

Cited by 7 publications

References 25 publications

Engineering Escherichia coli for Poly-β-hydroxybutyrate Production from Methanol

Engineering Escherichia coli for Poly-β-hydroxybutyrate Production from Methanol

Extracellular L-Asparaginase Synthesis Bacillus niacin Isolation, Optimization, and Characterization from Marine Saltern Sediment Sources

Statistical Design-Driven Optimization of Polyhydroxy-Butyrate (PHB) Production Using Sugarcane Bagasse by Brevibacterium sp. (PP989436) Strain

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