Microorganisms are capable to produce hydrogen during fermentation of organic substrates and industrial waste products can be used as feedstock for hydrogen producing bacteria. One of the substrates that can be effectively used for microbial hydrogen production is glycerol, which is a by-product from the process of biodiesel production, but glucose is mainly used as a model substrate. Different bacterial isolates were tested for hydrogen gas production rates from glucose and glycerol with test-systems constructed in our laboratory. Test-systems were optimised to allow adequate substrate and bacterial strain hydrogen productivity estimation in the liquid and gaseous phases. It was concluded that several of the isolated bacterial strains are suitable for bio-hydrogen production using glycerol as a substrate. Assessment was developed to establish whether microbial conversion of glycerol is an economically and environmentally viable possibility for bio-hydrogen production. The raw material cost noticeably decreases because of large quantities of available crude glycerol after biodiesel production and the highly reduced nature of carbon in glycerol per se.Key words: bio-hydrogen, fermentation, substrates, prototype bioreactor
INTRODUCTIONBiological production of hydrogen using bacteria is a promising and advantageous area, especially when hydrogen is gained from a variety of renewable resources [1,2]. Industrial and agricultural organic waste used as feedstock for hydrogen producing bacteria is a perspective way for alternative energy production and it noticeably decreases the raw material cost. During the conversion of organic wastes, in anaerobic environment, hydrogen gas is produced as a by-product. Substantial factors like availability and cost are highly important in the selection of waste materials to be used in hydrogen production with fermentative bacteria [3]. One of the substrates that can be effectively used for microbial hydrogen production is glycerol, which is a by-product from the process of biodiesel production. Because of large quantities available of crude glycerol and the highly reduced nature of carbon in glycerol per se, microbial conversion of it seems to be economically and environmentally viable possibility,
125Assessment of bio-hydrogen production from glycerol and glucose by fermentative bacteria especially because over the last several years the demand and production of biodiesel has remarkably increased [4,5]. Recently several authors have investigated hydrogen production using glycerol as a substrate by fermentative bacteria. Mangayil et al. [6] investigated optimal conditions (pH 6.5; 40 °C and 1 g/L raw glycerol) for hydrogen production using crude glycerol with microbial consortium mainly dominated by Clostridium species. Environmental conditions like medium pH and temperature are the major parameters to be controlled in the hydrogen production because they affect the qualitative and quantitative content of bacterial produced gas and the hydrogen yield and rate. Hydrogen production usi...